Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma

Huang, Cheng−Chen; Wang, Po-Hui; Lu, Yen‐Ting; Yang, Jyh Bin; Yang, Shun‐Fa; Ho, Yu‐Ting; Hsin, Chung‐Han

doi:10.3390/molecules25204851

Cited by 7 publications

(7 citation statements)

References 36 publications

(49 reference statements)

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“…[11] Additionally, it has substantial antitumor potential by regulating JNK, ERK, STAT3, and NFκB. [20][21][22] Because of the complexity of morusin, several investigations are needed to confirm the mechanism of therapeutic activity. Thus, we used bioinformatics which can streamline complicated processes to research the anticancer effect of morusin, forecast potential mechanisms and serve as the foundation and guide for subsequent in vitro or in vivo tests.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of morusin on breast cancer via network pharmacology and in vitro experiments

Xiao

Xue

et al. 2023

Medicine

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Background: This study aimed to investigate the therapeutic effect of morusin on breast cancer and decode its underlying molecular mechanism using network pharmacology and in vitro techniques. Methods: Swiss Target Prediction and PharMmapper were applied to screen morusin targets. The targets of human breast cancer were obtained from the GeneCards database, and the overlapping targets were screened. A protein-protein interaction network was constructed based on the overlapping targets by String and Cytoscape. Performed Gene Ontology enrichment as well as Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis on the shared targets of the drug and disease using the David database. Additionally, performed molecular docking using PyMoL and AutoDock software. Finally, the impact of morusin on breast cancer was demonstrated by cell experiments and western blot. Results: A total of 101 target genes were obtained through screening including ESR1, EGFR, ALB, CTNNB1, AKT1, and so on. Based on the annotation of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, the anticancer properties of morusin are linked to apoptosis, migration, and PI3K-AKT signaling pathways. Molecular docking showed an interaction between morusin and PIK3CA, AKT1. In vitro data demonstrated that morusin causes apoptosis and inhibits cell migration. Morusin also increased the expression of cleaved-PARP while decreasing the expression of p-PI3K and p-AKT. Conclusion: Through network pharmacology analysis and in vitro experiments, this study showed that morusin promotes apoptosis and inhibits migration by modulating the PI3K-AKT axis. Morusin plays a key role in the treatment of breast cancer.

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Section: Discussionmentioning

confidence: 99%

Mechanism of morusin on breast cancer via network pharmacology and in vitro experiments

Xiao

Xue

et al. 2023

Medicine

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“…Mitogen-activated protein kinases were reportedly associated with NPC cell invasion and migration, [19][20][21][22][23] and could regulate the functions of MMPs. 24,25 To determine whether DHM can affect the expressions of MAPKs, Western blotting assay was performed.…”

Section: Effects Of Dihydromyricetin On the Mitogenactivated Protein ...mentioning

confidence: 99%

Dihydromyricetin inhibits cancer cell migration and matrix metalloproteinases‐2 expression in human nasopharyngeal carcinoma through extracellular signal‐regulated kinase signaling pathway

Huang

Wang

et al. 2022

Environmental Toxicology

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Nasopharyngeal carcinoma (NPC) is endemic in Southeast Asia and the main cause of treatment failure is metastasis. A lot of biological and pharmacological actions of dihydromyricetin (DHM) have been reported such as regulating glucose and anticancer effects. The effects of DHM on the cancer invasion and migration of NPC, however, are still unclear. We therefore investigated the in vitro anti-metastatic properties of DHM on three human NPC cell lines (HONE-1, NPC-39, and NPC-BM), as well as the underlying signaling pathways. Our study revealed that DHM could suppress the migration and invasion in NPC cells. Gelatin zymography assay and western blotting assays demonstrated that DHM suppressed the enzyme activity and protein expression of matrix metalloproteinases-2 (MMP-2). Mitogen-activated protein kinases were also investigated to elucidate the signaling pathway, which showed that phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) was inhibited after the treatment of DHM. In conclusion, our data revealed that DHM inhibited the migration and invasion of NPC cells by suppressing the expression of MMP-2 via down regulating the ERK1/2 signaling pathway.

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“…According to a recent study, morusin can prevent human NPC HONE-1, NPC-39, and NPC-BM cells from migrating and invading. These findings showed that morusin is effective at preventing NPC invasion and metastasis ( Huang et al, 2020 ). In addition to being a tumor marker in NPC, MMP-2 is also linked to lymph node metastases and a poor prognosis ( Liu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 96%

“…MMP-2 is well known for degrading ECM as well as being a tumor marker for head and neck cancer with a bad prognosis. According to studies, morusin blocks the ability of cancer cells to migrate and invade by preventing the expression of the MMP-2 protein ( Huang et al, 2020 ). Previous research has shown that early MMP activity inhibition can be used to prevent cancer metastasis, and morusin may be a promising cancer therapeutic agent.…”

Section: Introductionmentioning

confidence: 99%

Exploring the therapeutic and anti-tumor properties of morusin: a review of recent advances

Hafeez

Najam

Armaghan

et al. 2023

Front. Mol. Biosci.

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Morusin is a natural product that has been isolated from the bark of Morus alba, a species of mulberry tree. It belongs to the flavonoid family of chemicals, which is abundantly present in the plant world and is recognized for its wide range of biological activities. Morusin has a number of biological characteristics, including anti-inflammatory, anti-microbial, neuro-protective, and antioxidant capabilities. Morusin has exhibited anti-tumor properties in many different forms of cancer, including breast, prostate, gastric, hepatocarcinoma, glioblastoma, and pancreatic cancer. Potential of morusin as an alternative treatment method for resistant malignancies needs to be explored in animal models in order to move toward clinical trials. In the recent years several novel findings regarding the therapeutic potential of morusin have been made. This aim of this review is to provide an overview of the present understanding of morusin’s beneficial effects on human health as well as provide a comprehensive and up-to-date discussion of morusin’s anti-cancer properties with a special focus on in vitro and in vivo studies. This review will aid future research on the creation of polyphenolic medicines in the prenylflavone family, for the management and treatment of cancers.

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Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma

Cited by 7 publications

References 36 publications

Mechanism of morusin on breast cancer via network pharmacology and in vitro experiments

Mechanism of morusin on breast cancer via network pharmacology and in vitro experiments

Dihydromyricetin inhibits cancer cell migration and matrix metalloproteinases‐2 expression in human nasopharyngeal carcinoma through extracellular signal‐regulated kinase signaling pathway

Exploring the therapeutic and anti-tumor properties of morusin: a review of recent advances

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