Morus nigra leaf extract improves glycemic response and redox profile in the liver of diabetic rats

Abstract

Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia and alterations in the carbohydrate, lipid, and protein metabolism. DM is associated with increased oxidative stress and pancreatic beta cell damage, which impair the production of insulin and the maintenance of normoglycemia. Inhibiting oxidative damage and controlling hyperglycemia are two important strategies for the prevention of diabetes. The pulp and leaf extracts of mulberry (Morus nigra L.) have abundant total phenolics and flavonoids, and its antioxidant potential may be an important factor for modulating oxidative stress induced by diabetes. In this study, DM was induced by intraperitoneal injection of alloxan monohydrate (135 mg kg(-1)). Female Fischer rats were divided into four groups: control, diabetic, diabetic pulp, and diabetic leaf extract. Animals in the diabetic pulp and diabetic leaf extract groups were treated for 30 days with M. nigra L. pulp or leaf extracts, respectively. At the end of treatment, animals were euthanized and, liver and blood samples were collected for analysis of biochemical and metabolic parameters. Our study demonstrated that treatment of diabetic rats with leaf extracts decreased the superoxide dismutase (SOD)/catalase (CAT) ratio and carbonylated protein levels by reducing oxidative stress. Moreover, the leaf extract of M. nigra L. decreased the matrix metalloproteinase (MMP)-2 activity, increased insulinemia, and alleviated hyperglycemia-induced diabetes. In conclusion, our study found that the leaf extract of M. nigra L. improved oxidative stress and complications in diabetic rats, suggesting the utility of this herbal remedy in the prevention and treatment of DM.

The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage

“…In addition, M. nigra leaf extract administration has been shown to protect liver tissue against the hepatotoxic effect of the antirheumatic drug methotrexate [24]. In recent studies, the extract of M. nigra leaf was shown to be capable of reducing oxidative stress and complications in the kidneys and liver of diabetic rats [25,26]. Our results are in accordance with previous studies that reported the hepatoprotective effect of M. nigra leaf extract.…”

BrazilianMorus nigraAttenuated Hyperglycemia, Dyslipidemia, and Prooxidant Status in Alloxan-Induced Diabetic Rats

“…It this way, Araujo et al [2015] [28] showed that long-term use of M. nigra extract raised insulinemia in diabetic rats. Insulin-induced suppression of endogenous glucose production, which is an essential mechanism to normoglycemia, is affected in DM chronically [27].…”

“…Previous works have shown that aqueous extract of M. nigra leaves raised SOD activity and reduced oxidative stress in normal and hyperglycemic rats [11, 28]. …”

Effects of simulated gastrointestinal digestion on the physicochemical properties, erythrocyte haemolysis inhibitory ability and chemical antioxidant activity of mulberry leaf protein and its hydrolysates

“…Nowadays, mulberry leaf not only is regarded as useful herbal medicines, but also is consumed as functional foods or food nutritional additives to produce mulberry leaf tea and beverage (Wanyo et al, 2011;Phoonan et al, 2014;Thirugnanasambandham et al, 2015). Recent investigations on the chemical constituents, and corresponding bioactivities, of mulberry leaf have indicated that antioxidant activity plays an important role in its therapeutic effects (Park et al, 2013;Araujo et al, 2015;Chang et al, 2016). We have previously focused on the extraction of mulberry leaf protein (MP) and on the determination of its physicochemical properties and antioxidant activities (Sun et al, 2015(Sun et al, , 2016.…”