Morus alba Stem Extract Suppresses Matrix Metalloproteinases (MMP)-1, MMP-9, and Tissue Inhibitors of Metalloproteinase (TIMP)-1 Expression via Inhibition of IκBα Degradation Induced by Porphyromonas gingivalis LPS Signal in THP-1 Cells

Yiemwattana, Ichaya; Kaomongkolgit, Ruchadaporn; Wirojchanasak, Sodsi; Chaisomboon, Niratcha

doi:10.1055/s-0039-1694314

Cited by 9 publications

(7 citation statements)

References 18 publications

(21 reference statements)

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“…Our findings are consistent with some other studies showing that IL-6 and TNF-α were increased by LPS in macrophages [49,50]. The inflammatory response can be orchestrated by pro-inflammatory cytokines such as TNFα and IL-6 [25,51,52] and it can be mediated through NF-κB transcriptional factor [44,52]. Additionally, ROS can be regulated by NF-kB [53].…”

Section: Discussionsupporting

confidence: 92%

“…Especially, P. gingivalis-LPS is a strong virulence factor in periodontitis and induces cytokine secretion (TNF-α, IL-6, and MCP-1) and inflammatory responses via TLRs [43]. The results of our study are consistent to various other research showing anti-inflammatory effects of MAE in P. gingivalis-infected THP-1 cells [44]. In addition to P. gingivalis, F. nucleatum is another keystone periodontal pathogen.…”

Section: Discussionsupporting

confidence: 91%

“…For example, the component (1-deoxynojirimycin (DNJ)) of MAE showed anti-adhesive effects by controlling the overgrowth and inhibiting biofilm formation of S. mutans [9]. Additionally, MAE have anti-inflammatory effects by suppressing MMPs, tissue destruction-related protein, in P. gingivalis LPS-infected THP-1 cells [44]. Taken together, MAE could function as anti-bacterial and anti-inflammatory agents in oral microorganisms.…”

Section: Discussionmentioning

confidence: 99%

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Anti-Inflammatory and Anti-Bacterial Potential of Mulberry Leaf Extract on Oral Microorganisms

Kim

Kang

2022

IJERPH

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Mulberry leaves extract (Morus alba extracts; MAE) is known to have therapeutic potentials for numerous human diseases, including diabetes, neurological disorders, cardiovascular diseases, and cancers. However, there has not been sufficient research proving therapeutic effects on oral disease and its related oral risk factors. Thus, we investigated whether MAE has any anti-inflammatory and anti-bacterial effects on risk factors causing oral infectious diseases. To examine the anti-inflammatory response and bacterial inhibition of MAE, we measured intracellular reactive oxygen species (ROS) generation, production of pro-inflammatory cytokines, and the bacterial growth rate. Our study showed that MAE has anti-inflammatory activities, which inhibit the ROS generation and suppressed the production of pro-inflammatory cytokines (TNF-α and IL-6) in human monocyte THP-1 cells by stimulating lipopolysaccharide (LPS) and/or F. nucleatum, which are the virulent factors in periodontal diseases. Furthermore, MAE inhibited the bacterial growth on oral microorganisms (F. nucleatum and S. mutans) infected THP-1 cells. These findings suggested that MAE could be a potential natural source for therapeutic drugs in oral infectious disease.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Anti-Inflammatory and Anti-Bacterial Potential of Mulberry Leaf Extract on Oral Microorganisms

Kim

Kang

2022

IJERPH

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“…LPS probably activate ERK1/2 pathway and JNK pathway and lead to MMP-9 expression [27]. LPS-induced matrix metalloproteinases (MMP)-1, MMP-9 expression in THP-1 cells [28]. Recent study demonstrated that LPS promoted the production of IL-1β and TNF-α in response to infection mediated by a mechanism for A2AR targeting in microglia [29].…”

Section: Discussionmentioning

confidence: 99%

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

et al. 2020

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Background: Cell proliferation and death are key components of wound healing and tissue repair. Telocytes (TCs) represent a newly discovered cell type that can protect tissue from acute injury via cell-cell communication with adjacent cells. The aim of this study was to use a mouse model of skin wound healing and lipopolysaccharide (LPS)induced cell injury to evaluate the effects of TCs on skin wound healing in vivo and in vitro. Material/methods: Immunohistochemical staining was performed to evaluate the alteration of TCs in tissues from normal and chronic wound patients. Then, a male C57BL/6 mouse wound model of the back was established. The mice were divided randomly into three groups, and wound healing was estimated according to the wound healing rate and histology. An LPS-induced co-culture model of a mouse lung telocyte cell line (TCs) with human keratinocyte (HaCaT), human dermal microvascular endothelial cell (HDMEC) or murine fibroblast (L929) cell lines was established to analyse the effects of TCs on constitutive cell types of the skin. Cell proliferation, migration and apoptosis were examined, and reactive oxygen species (ROS) and inflammatory factors in HaCaT cells, HDMECs, and L929 cells were detected to study the mechanisms involved in TC protection in skin wounds. Results: TCs were significantly increased in tissues from chronic wound patients compared with healthy controls. Wound healing was significantly improved in wound mouse models treated with exogenous TCs compared with LPSinduced models. TCs reversed the LPS-induced inhibition of HaCaT cells and HDMECs and reduced the LPS-induced apoptosis of HaCaT cells and the death ratios of HDMECs and L929 cells. TCs reversed LPS-induced ROS in HDMECs and L929 cells and decreased inflammatory factor mRNA levels in HaCaT cells, HDMECs and L929 cells. Conclusions: TCs reduce wound healing delay, and inflammatory responses caused by LPS might be mediated by inflammatory inhibition, thus restricting apoptosis and promoting migration of the main component cell types in the skin.

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“…Antimicrobial, antioxidant, anti-diabetic, anti-hyperlipidemic, anti-atherosclerotic, anti-obesity, cardioprotective, and cognitive enhancement activities of Folium Mori have been reported ( Chan et al, 2016 ). Furthermore, efficacy against airway inflammation (by interruption of NF-κB and JNK/c-Jun signaling) by the Morus constituent Moracin M ( Lee et al, 2016 ) and inhibition of metalloproteinases were seen ( Yiemwattana et al, 2019 ; Wongwat et al, 2020 ). Antiviral activities of Morus alba juice and seed (Kim et al, 2018) as well as specifically activity against human corona virus of Folium Mori have been observed ( Thabti et al, 2020 ).…”

Section: Morus Alba (Folium Mori)mentioning

confidence: 99%

Toxicity as prime selection criterion among SARS-active herbal medications

2021

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Morus alba Stem Extract Suppresses Matrix Metalloproteinases (MMP)-1, MMP-9, and Tissue Inhibitors of Metalloproteinase (TIMP)-1 Expression via Inhibition of IκBα Degradation Induced by Porphyromonas gingivalis LPS Signal in THP-1 Cells

Cited by 9 publications

References 18 publications

Anti-Inflammatory and Anti-Bacterial Potential of Mulberry Leaf Extract on Oral Microorganisms

Anti-Inflammatory and Anti-Bacterial Potential of Mulberry Leaf Extract on Oral Microorganisms

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

Toxicity as prime selection criterion among SARS-active herbal medications

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