Morus alba and active compound oxyresveratrol exert anti-inflammatory activity via inhibition of leukocyte migration involving MEK/ERK signaling

Abstract

BackgroundMorus alba has long been used in traditional Chinese medicine to treat inflammatory diseases; however, the scientific basis for such usage and the mechanism of action are not well understood. This study investigated the action of M. alba on leukocyte migration, one key step in inflammation.MethodsGas chromatography-mass spectrometry (GC-MS) and cluster analyses of supercritical CO2 extracts of three Morus species were performed for chemotaxonomy-aided plant authentication. Phytochemistry and CXCR4-mediated chemotaxis assays were used to characterize the chemical and biological properties of M. alba and its active compound, oxyresveratrol. fluorescence-activated cell sorting (FACS) and Western blot analyses were conducted to determine the mode of action of oxyresveratrol.ResultsChemotaxonomy was used to help authenticate M. alba. Chemotaxis-based isolation identified oxyresveratrol as an active component in M. alba. Phytochemical and chemotaxis assays showed that the crude extract, ethyl acetate fraction and oxyresveratrol from M. alba suppressed cell migration of Jurkat T cells in response to SDF-1. Mechanistic study indicated that oxyresveratrol diminished CXCR4-mediated T-cell migration via inhibition of the MEK/ERK signaling cascade.ConclusionsA combination of GC-MS and cluster analysis techniques are applicable for authentication of the Morus species. Anti-inflammatory benefits of M. alba and its active compound, oxyresveratrol, may involve the inhibition of CXCR-4-mediated chemotaxis and MEK/ERK pathway in T and other immune cells.

Effect of solar radiation on the functional components of mulberry (Morus alba L.) leaves

“…There are many reports on the uses of mulberry leaves, such as the suppression of hyperglycemia and atherosclerosis, as well as their antioxidant, antineoplastic, anti‐inflammatory and anti‐obesity effects. 1‐Deoxynojirimycin (DNJ), a functional component of mulberry, is known to inhibit the elevation of blood glucose levels.…”

Ionic Liquid-based Microwave-Assisted Extraction (Il-MAE) of Oxyresveratrol from Morus alba Roots

“…This result was compared with the previous study of oxyresveratrol extraction on literature, as seen in Table 2 EA fraction of M. alba branch yield was 1.89% (Chen et al, 2013). Oxyresveratrol from barks and roots methanolic extract calculated with HPTLC method was 0.27%, and 0.30%, and was not identified in leaves, fruits, or xylem (Ayinampudi et al, 2011) and from stems ethanolic extract calculated with HPLC was 17.87 ± 0.61% with yield 5.13% (Zeng et al, 2010).…”

FOXO3a Activation by oxyresveratrol ofMorus bombyciskoidzumi extract mediates antioxidant activity

“…Recently, it has been reported that oxyresveratrol has an anti-inflammatory activity through inhibition of leukocyte migration. This is caused by inhibition of MEK/ERK pathway (Chen et al 2013). In addition, oxyresveratrol has a neuroprotective activity and inhibits the apoptotic cell death in transient rat middle cerebral artery occlusion (MCAO) model of brain ischemia.…”