Background: The novel coronavirus disease 2019 (COVID-19) pandemic has become a challenge for nursing homes in China. Nursing homes are particularly dangerous places regarding the spread of COVID-19 given that they house vulnerable, high-risk populations. As such, several useful guidelines for coping with COVID-19 in nursing homes have been provided. However, the actual implementation rates of such guidelines are unknown. This study aims to document the adherence of nursing homes to the Ministry of Civil Affairs guidelines for COVID-19 prevention and control in nursing homes.Methods: A cross-sectional study was conducted among 484 nursing homes in 136 cities of 28 provinces in China. A self-report questionnaire was created based on the Ministry of Civil Affairs guidelines for COVID-19 prevention and control in nursing homes (first edition). The questionnaire and the Transformational Leadership in the Public Sector Scale were sent to nursing home managers via the Wenjuanxing app online from February 7 to 29, 2020. A total of 461 responses were included in the analysis. Results: The average overall implementation rate of COVID-19 prevention and control measures was 80.0% (143.97/180). The average implementation rates for hygienic behaviour management, access management were lower, with 75.3% and 78.7% respectively. The number of medical staff, established a quarantine unit (room), leadership’s three dimensions included intellectual stimulation, individualized consideration, and idealized influence were found to affect the prevention and control implementation (p<0.05). Nursing homes that did not have hospital-nursing home cooperation, did not have medical staff, and had not established a quarantine unit (room) had low implementation rates (p<0.01). 69.8% (332/461) of nursing home managers reported they had serious resource problems, inadequate of protective supplies (72.0%) and staff shortage (47.7%) were two primary problems. Conclusion: Overall, the implementation of prevention and control measures by nursing homes are insufficient during the epidemic in China. Further education for staff, provide support to staff, supply adequate protective resources, and cooperation with hospitals are required to improve the implementation rate. It is urgent for nursing homes keep the safety of the residents and staff.