Mortality and histopathology in sheepshead minnow (Cyprinodon variegatus) larvae exposed to pectenotoxin-2 and Dinophysis acuminata

Gaillard, Sylvain; Réveillon, Damien; Mason, Patrice L.; Ayache, Nour; Sanderson, Marta P.; Smith, Juliette L.; Giddings, Sabrina D.; McCarron, Pearse; Sechet, Véronique; Hégaret, Hélène; Heß, Philipp; Vogelbein, Wolfgang K.

doi:10.1016/j.aquatox.2023.106456

Cited by 7 publications

(4 citation statements)

References 62 publications

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“…Protein phosphatases have been well studied in ecotoxicology, especially PP1 and PP2A, which are known to be inhibited by marine toxins such as OA and microcystin-LR (MC-LR) [75]. In the present study, PP activity was detected in control embryos from 8 hpf onwards (i.e., before hatching; Figures 3A and 4), which was also observed in a previous study of S. rivoliana embryos [35]. Similarly, Shi et al [76] found pre-hatching phosphatase activity in olive flounder (Paralichthys olivaceus) embryos.…”

Section: Regulation Of Protein Phosphatasessupporting

confidence: 89%

“…The use of marine fish embryos and larvae as sentinels to detect the modes of action of marine toxins has been proposed in many studies mostly conducted with Zebrafish (Danio rerio) [19,20]. Previous studies have reported the negative effects of toxins on fish larvae [34,35]. In S. rivoliana, exposure to diarrhetic shellfish toxins (DSTs; okadaic acid [OA] and dinophysistoxin-1 [DTX-1]), PSTs, and saxitoxin analogs (gonyautoxin 2-3 [GTX 2-3], decarbamoyl gonyautoxin 2-3 [dc-GTX2-3], and C1-C2) has been recently shown to deregulate embryogenesis-related pathways, inhibit phosphatase activity, and induce metabolic responses in larvae by increasing gene expression and lipid metabolism enzyme activity [36,37].…”

Section: Introductionmentioning

confidence: 99%

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Toxicogenomic Effects of Dissolved Saxitoxin on the Early Life Stages of the Longfin Yellowtail (Seriola rivoliana)

Guinle,

Núñez-Vázquez,

Fernández-Herrera

et al. 2023

Marine Drugs

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Harmful algal blooms (HABs) can produce a variety of noxious effects and, in some cases, the massive mortality of wild and farmed marine organisms. Some HAB species produce toxins that are released into seawater or transferred via food webs (particulate toxin fraction). The objective of the present study was to identify the toxicological effects of subacute exposure to saxitoxin (STX) during embryonic and early larval stages in Seriola rivoliana. Eggs were exposed to dissolved 19 STX (100 μg L−1). The toxic effects of STX were evaluated via the hatching percentage, the activity of three enzymes (protein and alkaline phosphatases and peroxidase), and the expression of four genes (HSF2, Nav1.4b, PPRC1, and DUSP8). A low hatching percentage (less than 5%) was observed in 44 hpf (hours post fertilization) embryos exposed to STX compared to 71% in the unexposed control. At this STX concentration, no oxidative stress in the embryos was evident. However, STX induced the expression of the NaV1.4 channel α-subunit (NaV1.4b), which is the primary target of this toxin. Our results revealed the overexpression of all four candidate genes in STX-intoxicated lecithotrophic larvae, reflecting the activation of diverse cellular processes involved in stress responses (HSF2), lipid metabolism (PPRC1), and MAP kinase signaling pathways associated with cell proliferation and differentiation (DUSP8). The effects of STX were more pronounced in young larvae than in embryos, indicating a stage-specific sensitivity to the toxin.

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Section: Regulation Of Protein Phosphatasessupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Toxicogenomic Effects of Dissolved Saxitoxin on the Early Life Stages of the Longfin Yellowtail (Seriola rivoliana)

Guinle,

Núñez-Vázquez,

Fernández-Herrera

et al. 2023

Marine Drugs

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“…The research to disclose other secondary metabolites responsible for P. parvum's toxicity is still in progress [44]. Many species of Prymnesium are recognized to produce toxic compounds that impact erythrocytes and gill-respiring organisms [45]. P. parvum causes periodical HABs, whose cell densities grow quickly and are able to release powerful ichthyotoxins [44].…”

Section: Prymnesium Parvummentioning

confidence: 99%

Review of Harmful Algal Blooms (HABs) Causing Marine Fish Kills: Toxicity and Mitigation

Oh,

Pushparaj,

Muthu

et al. 2023

Plants

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Extensive growth of microscopic algae and cyanobacteria results in harmful algal blooms (HABs) in marine, brackish, and freshwater environments. HABs can harm humans and animals through their toxicity or by producing ecological conditions such as oxygen depletion, which can kill fish and other economically or ecologically important organisms. This review summarizes the reports on various HABs that are able to bring about marine fish kills. The predominant HABs, their toxins, and their effects on fishes spread across various parts of the globe are discussed. The mechanism of HAB-driven fish kills is discussed based on the available reports, and existing mitigation methods are presented. Lapses in the large-scale implementation of mitigation methods demonstrated under laboratory conditions are projected. Clay-related technologies and nano-sorption-based nanotechnologies, although proven to make significant contributions, have not been put to use in real-world conditions. The gaps in the technology transfer of the accomplished mitigation prototypes are highlighted. Further uses of remote sensing and machine learning state-of-the-art techniques for the detection and identification of HABs are recommended.

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“…The active compounds, identified as groups of polyethers, notably include okadaic acid (OA), pectenotoxin (PTX), and their derivatives. In sufficient amounts, the toxins of the okadaic acid group cause severe gastroenteritis in humans (Yasumoto et al, 1985) while pectenotoxin-2 has been shown to negatively impact fertilisation success of oyster gametes (Gaillard et al, 2020) and development of fish embryos (Gaillard et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Effects of copepod chemical cues on intra- and extracellular toxins in two species ofDinophysis

Pourdanandeh,

Séchet,

Carpentier

et al. 2024

Preprint

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Copepods may contribute to harmful algal bloom formation by selectively rejecting harmful cells. Additionally, copepods and the chemical cues they exude, copepodamides, have been shown to induce increased toxin production in paralytic and amnesic toxin producing microalgae. However, it is unknown if diarrhetic shellfish toxin (DST) producers such asDinophysisrespond to copepods or copepodamides in a similar fashion. Here we expose laboratory cultures ofDinophysis sacculusandD. acuminatato direct grazing byAcartiasp. copepods or copepodamides and measure their toxins after three days. Total DSTs increased by 8 - 45% inD. sacculusbut was significantly different from controls only in the highest (10 nM) copepodamide treatment whereas toxin content was not affected inD. acuminata. Growth rate was low across all groups and explained up to 91% of the variation in toxin content. DSTs were redistributed from internal compartments to the extracellular medium in the highest copepodamide treatments (5 - 10 nM), which were two to three times higher than controls and indicates an active release or passive leakage of toxins. Untargeted analysis of endometabolomes indicated significant changes in metabolite profiles for both species in response to the highest copepodamide treatments, independent of known toxins. However, it is not clear whether these are stress responses or caused by more complex mechanisms. The relatively small grazer-induced effect inDinophysisobserved here, compared to several species ofAlexandriumandPseudo-nitzschiareported previously, suggests that DST production inDinophysisis likely not induced by copepods, except perhaps in patches with high copepod densities. DSTs may, however, represent a constitutive chemical defence forDinophysis, or serve an altogether different purpose.

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Mortality and histopathology in sheepshead minnow (Cyprinodon variegatus) larvae exposed to pectenotoxin-2 and Dinophysis acuminata

Cited by 7 publications

References 62 publications

Toxicogenomic Effects of Dissolved Saxitoxin on the Early Life Stages of the Longfin Yellowtail (Seriola rivoliana)

Toxicogenomic Effects of Dissolved Saxitoxin on the Early Life Stages of the Longfin Yellowtail (Seriola rivoliana)

Review of Harmful Algal Blooms (HABs) Causing Marine Fish Kills: Toxicity and Mitigation

Effects of copepod chemical cues on intra- and extracellular toxins in two species ofDinophysis

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