Morphostructural Analysis of Human Follicular Stem Cells on Highly Porous Bone Hydroxyapatite Scaffold

Abstract

In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3–6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.

Substance P Upregulates LTB4 in Rat Adherent Macrophages from Granuloma Induced by KMnO4

“…The ability of SP to increase the expression of LTB 4 in RAGMs highlights the relationship between neuropeptides and chronic inflammation in the development of this pathophysiological event (Keeble and Brain 2004;Angelini et al 2007;Inoue et al 2007). The present data may prove and suggest, by providing strong evidence, that SP, an inflammatory neuropeptide, contributes to cell aggregation, chemokinetics and chemotactic activity by inducing the transcription of 5-LO and the generation of LTB4 in chronic inflammatory calcified tissue (Anogianaki et al, 2007;Mori et al 2007;Tetè et al 2007;Magarelli et al 2007). Thus, inhibition of SP may represent a potential therapeutic approach for the treatment of immunological diseases associated with inflammation, where LTB 4 is present.…”

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

“…On the basis of the present results, oAEC could be considered as a useful source of progenitor cells able to conjugate a good mesenchymal plasticity [25], [44], [46], [48], [49] with other useful biological properties. In fact, AEC are uncontroversial and largely available, are stable and can be easily expanded in vitro , thus overcoming the practical limitation still linked to the use of the mesenchymal stromal cells (MSC) isolated from adult tissues such as dental pulp, periodontal ligament, dental follicle and bone marrow aspirates [20], [22], [23], [75]–[82]. Furthermore, oAEC confirmed pro-angiogenetic activity and no tumurogenic attitude [29], [44], [47], [54], [83].…”

A cell‐free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

“…It must be noted that the time of evolution of the regeneration prior to the sacrifice of the rabbits was 12 weeks. This time was decided as it is within the range established by the ICRS (Zhu et al, ), and longer times do not seem to improve the quality of cartilage regeneration (Kumar, Muzzarelli, Muzzarelli, Sashiwa, & Domb, ; Teté et al, ). Nevertheless, it is shorter than the half‐life of both PLLA and CHT, so the presence of the biomaterial in the samples was expected.…”