Morphophysiological responses of detached and adhered biofilms of Pseudomonas fluorescens to acidic electrolyzed water

Cai, Liuyang; Hu, Hao; Lu, Qingyi; Wang, Huhu; Xu, Xinglian; Zhou, Guanghong; Kang, Zhuang‐Li; Ma, Haile

doi:10.1016/j.fm.2019.01.007

Cited by 19 publications

(7 citation statements)

References 50 publications

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“…PALA therapy may have depleted cytoplasmic ATP because of decreased ATP synthesis or increased ATP hydrolysis by the proton-pump ATPase as a result of an accumulation of reactive oxygen species within the cell [ 40 ]. Cai et al [ 41 ] proposed that AEW treatment decreasing the intracellular ATP concentration might be partially explained by the enhanced cell membrane permeability and membrane impairment under AEW, leading to leakage of ATP and an altered balance of cations, such as H + and Na + . Our previous experiments showed that membrane completeness and permeability were slightly altered, and the leakage rate of small ions, such as the K + of cells, was much lower when compared to a reduction in the ATP concentration when cells were exposed to the PALA (0.20%) treatment.…”

Section: Resultsmentioning

confidence: 99%

Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid against Pseudomonas ludensis by Untargeted Metabolomics

Wang

Sheng

et al. 2023

Foods

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Plasma-activated liquid is a novel non-thermal antibacterial agent against a wide spectrum of foodborne bacteria, yet fewer studies focused on its disinfection of meat spoilage bacteria. In this study, the antibacterial properties of plasma-activated lactic acid (PALA) on Pseudomonas lundensis, isolated and identified from spoilage beef, were investigated. A plasma jet was used to treat lactic acid (0.05–0.20%) for 60–120 s. The results presented that the 0.2% LA solution treated with plasma for 120 s caused a 5.64 log reduction. Additionally, the surface morphology, membrane integrity and permeability were altered slightly and verified by scanning electron microscopy, double staining of SYTO-9 and propidium iodide, and a K+ test kit. The intracellular organization of the cells, observed by transmission electron microscopy, was damaged significantly. Increased intracellular reactive oxygen species (ROS) levels exceeded the antioxidant ability of glutathione (GSH), leading to a reduction in the activity of malate dehydrogenase (MDH), succinic dehydrogenase (SDH) and intracellular ATP levels. Metabolomics analysis indicated that the energy and synthesis of essential components, such as DNA and amino acid-related metabolic pathways, were disturbed. In conclusion, this research established a theoretical basis for the use of PALA in refrigerated beef preservation by shedding light on the bacteriostatic effect of PALA against Pseudomonas lundensis.

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Section: Resultsmentioning

confidence: 99%

Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid against Pseudomonas ludensis by Untargeted Metabolomics

Wang

Sheng

et al. 2023

Foods

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“…Intracellular pH was determined using the pH sensitive dye BCECF-AM (Invitrogen) using a protocol established for measuring intracellular pH in bacteria [ 21 ]. Cells were resuspended in PBS containing 1 μM BCECF-AM, followed by 30 min incubation at 37°C [ 21 ], washed in PBS to remove extracellular dye, and subsequently resuspended in 5 mL PBS. Fluorescence was measured using an excitation wavelength of 490nm and an emission wavelength of 530 nm on the (PHERAstar spectrophotometer, BMG LabTech).…”

Section: Methodsmentioning

confidence: 99%

The central role of arginine in Haemophilus influenzae survival in a polymicrobial environment with Streptococcus pneumoniae and Moraxella catarrhalis

Tikhomirova

Zilm²,

Trappetti³

et al. 2022

PLoS ONE

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Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis are bacterial species which frequently co-colonise the nasopharynx, but can also transit to the middle ear to cause otitis media. Chronic otitis media is often associated with a polymicrobial infection by these bacteria. However, despite being present in polymicrobial infections, the molecular interactions between these bacterial species remain poorly understood. We have previously reported competitive interactions driven by pH and growth phase between H. influenzae and S. pneumoniae. In this study, we have revealed competitive interactions between the three otopathogens, which resulted in reduction of H. influenzae viability in co-culture with S. pneumoniae and in triple-species culture. Transcriptomic analysis by mRNA sequencing identified a central role of arginine in mediating these interactions. Arginine supplementation was able to increase H. influenzae survival in a dual-species environment with S. pneumoniae, and in a triple-species environment. Arginine was used by H. influenzae for ATP production, and levels of ATP generated in dual- and triple-species co-culture at early stages of growth were significantly higher than the combined ATP levels of single-species cultures. These results indicate a central role for arginine-mediated ATP production by H. influenzae in the polymicrobial community.

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“…There was no significant difference in the ability of bacteria adhering to the nonrust steel and glass, which also confirms the previous conclusion. Cai et al [27]…”

Section: Sem Images Of Combined Enzymes-inoculated Carriersmentioning

confidence: 99%

“…There was no significant difference in the ability of bacteria adhering to the nonrust steel and glass, which also confirms the previous conclusion. Cai et al [27] reported that the acidic electrolytic water can effectively inhibit the formation of fluorescent Pseudomonas biofilm. The results of SEM images showed that the fluorescent Pseudomonas biofilm treated by acidic electrolytic water was separated and decomposed into small extracellular matrix aggregates, and the amount of extracellular matrix and bacterial adhesion was significantly reduced compared with the untreated group.…”

Section: Sem Images Of Combined Enzymes-inoculated Carriersmentioning

confidence: 99%

Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of Vibrio parahaemolyticus Biofilm: Mechanism on cpsA-J Expression and Application on Different Carriers

Dong

et al. 2022

Foods

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Vibrio parahaemolyticus is a human foodborne pathogen, and it can form a mature biofilm on food and food contact surfaces to enhance their resistance to antibacterial agents. In this study, the effect of anti-biofilm enzymes (combined lipase, cellulase and proteinase K) on the inhibition and eradication of pathogen biofilm was evaluated. The biofilm content of V. parahaemolyticus showed the highest level at the incubation time of 24 h, and the combined enzymes significantly inhibited the biofilm’s development. The biofilm’s inhibition and eradication rate at an incubation time of 24 h was 89.7% and 66.9%, respectively. The confocal laser scanning microscopic images confirmed that the microcolonies’ aggregation and the adhesion of biofilm were inhibited with the combined enzyme treatment. Furthermore, combined enzymes also decreased the concentration of exopolysaccharide (EPS) and disrupted the EPS matrix network, wherein the expression of the EPS-related gene, cpsA-J, was likewise suppressed. The combined enzymes showed an excellent inhibition effect of V. parahaemolyticus biofilm on different carriers, with the highest inhibition rate of 59.35% on nonrust steel plate. This study demonstrates that the combined enzyme of lipase, cellulase and proteinase K could be a novel candidate to overcome bioﬁlm’s problem of foodborne pathogens in the food industry.

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Morphophysiological responses of detached and adhered biofilms of Pseudomonas fluorescens to acidic electrolyzed water

Cited by 19 publications

References 50 publications

Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid against Pseudomonas ludensis by Untargeted Metabolomics

Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid against Pseudomonas ludensis by Untargeted Metabolomics

The central role of arginine in Haemophilus influenzae survival in a polymicrobial environment with Streptococcus pneumoniae and Moraxella catarrhalis

Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of Vibrio parahaemolyticus Biofilm: Mechanism on cpsA-J Expression and Application on Different Carriers

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