Morphometry in histopathology

Hamilton, Peter; Allen, Derek C.

doi:10.1002/path.1711750403

Cited by 68 publications

(44 citation statements)

References 61 publications

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“…Therefore, computerized morphometry is more reproducible and increases its potential diagnostic value. 5,9,11,13 In the present study, the cytopathologic morphometric data indicated a correlation between MNA and patient survival; Kaplan-Meier analysis demonstrated that survival times were significantly shorter as nuclear area increased. These results corroborate previous data that demonstrate a relationship between morphometric analysis and histopathologic grading 22,34 and confirm the usefulness of cytomorphometry alone as a predictor of survival.…”

Section: Discussionsupporting

confidence: 57%

Nuclear Morphometry in Cytopathology: A Prognostic Indicator for Canine Cutaneous Mast Cell Tumors

et al. 2009

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Abstract. Twenty-nine canine cutaneous mast cell tumors (MCTs) were morphometrically analyzed with regard to mean nuclear area (MNA) using cytopathology smears. The results showed a correlation between MNA and survival. When graded into 2 morphometrically different groups, there were statistically significant differences among high-and low-grade MCTs, regarding both Romanowsky-type stain and hematoxylin and eosin. Cytomorphometry could also separate histologic grade II tumors with better prognosis from the more aggressive MCTs. The results indicated that nuclear morphometry on cytopathology preparations can predict the biological behavior of cutaneous MCTs in dogs in an independent manner, yielding a rapid and reproducible diagnosis, which renders the method useful for veterinary oncology.

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Section: Discussionsupporting

confidence: 57%

Nuclear Morphometry in Cytopathology: A Prognostic Indicator for Canine Cutaneous Mast Cell Tumors

et al. 2009

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“…Those of FITC-dextran (70, 4 kDa) having similar molecular weights to FITC-albumin and FITC-insulin, respectively, were extremely low. Using cell number/mg cell protein estimated by the measurement of DNA content in each type of cell as reported previously (1322ϫ10 4 General Characteristics of Insulin Uptake by Type II and Type I-Like Cells In the following experiments, the amount of FITC-insulin taken up into the cells (uptake) was estimated by subtracting the cell surface binding at 4°C from the total cell association at 37°C. Figure 1A shows the time course of FITC-insulin uptake by type II and type I-like cells.…”

Section: Comparison Of Apparent Uptake Clearance Of Variousmentioning

confidence: 99%

“…3,4) Recently, the lung has attracted a great deal of interest as an alternative administration route for protein and peptide drugs. 3,5) The inhalation pulmonary delivery system, which may enable the systemic absorption of protein and peptide drugs, has been extensively studied.…”

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confidence: 99%

Mechanism of Insulin Uptake in Rat Alveolar Type II and Type I-Like Epithelial Cells

Ikehata

Yumoto

Kato

et al. 2009

Biological & Pharmaceutical Bulletin

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The alveolar epithelium is comprised of type II and type I cells, which are two morphologically and functionally different epithelial cells. Alveolar type I cells, which are squamous and cover more than 90% of the alveolar surface area, play a major role in gas exchange. 1,2) On the other hand, alveolar type II cells are cuboidal and outnumber type I cells, although type II cells occupy less than 10% of the surface area even when their apical microvilli are taken into consideration.1,2) In addition, type II cells have multiple functions such as surfactant production and secretion, and serve as type I cell progenitors.2)The majority of protein and peptide therapeutic drugs were developed as injection formulations, and have a variety of problems, especially for patients, in terms of safety, pain, and needle phobia. 3,4) Recently, the lung has attracted a great deal of interest as an alternative administration route for protein and peptide drugs. 3,5) The inhalation pulmonary delivery system, which may enable the systemic absorption of protein and peptide drugs, has been extensively studied.6) In particular, clinical research on inhaled insulin has confirmed the efficacy and safety of inhaled insulin in the treatment of diabetes mellitus. 7) In addition, the bioavailability of inhaled insulin via the lung is greater compared to other non-invasive routes such as the gut and nose. 8)Previous studies concerning protein transport across alveolar epithelia have suggested that the transport system most likely to be involved is endocytosis. 9) We also have studied the transport of albumin in alveolar epithelial cells, and found that albumin is internalized into type II and type I cells via a clathrin-mediated endocytic pathway with greater uptake activity in type II cells than in type I cells.10) These findings indicate that despite the much smaller surface area of type II cells compared to type I cells, type II cells most likely play an important role in the endocytic transport of proteins in alveolar epithelia.In the case of small proteins (peptides) such as insulin, both paracellular and transcellular routes may be involved in alveolar epithelial transport.1) Bur et al. 11) reported that in human primary cultured alveolar type I-like epithelial cells, the apparent permeability coefficient of insulin was comparable to that reported for dextran, indicating that a specific, transcellular transport process is not involved. On the other hand, the alveolar epithelial junction was reported to be tighter than other epithelia, 1) suggesting that the contribution of a paracellular route for insulin in alveolar epithelial transport may not be so high. Furthermore, it has been reported that megalin serves as an endocytic receptor for insulin uptake in rat renal proximal tubule cells, 12) and that megalin is also expressed in alveolar epithelial cells.13) Thus, it is possible that an endocytic pathway is involved in insulin transport in alveolar epithelia. However, little information is available concerning the handling of insu...

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“…1,3,11,16,18,30 One of the solutions found over the last few decades to minimize subjective interobserver variations and consequently aid diagnostic decisions was the development of computerized morphometric techniques. 5,7,8,12,15,17,22 An additional relevant method is cytopathologic examination, which is an efficient, practical, and rapid technique for the diagnosis of mast cell tumors. 6,7,13,19 Using cytopathology is also advantageous in that the cell is spread on the slide so that its image is a complete projection of its cytoplasm, thereby eliminating the tridimensional problems of the various sections of the same cell encountered in histopathology.…”

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confidence: 99%

Morphometry of Canine Cutaneous Mast Cell Tumors

2003

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Abstract. Twenty-four canine cutaneous nodules, diagnosed as mast cell tumors by fine-needle aspiration biopsy and confirmed by histopathologic analysis by staining with hematoxylin and eosin (HE) and toluidine blue, were analyzed by computerized nuclear morphometry on panoptic-and HE-stained cytopathology slides. Two hundred nuclei per lesion were examined. The morphometric parameters investigated were nuclear area, mean diameter, perimeter, regularity factor, and ellipticity factor. Lesions were graded as I (well differentiated), II (intermediate differentiation), or III (poorly differentiated) according to the following morphologic features: invasiveness, cellularity and cellular morphology, mitotic index, and stromal reaction. Nuclear morphometric results were then compared with histopathologic grades. Values of nuclear area, mean diameter, and perimeter increased with increase in histopathologic grade, but statistical analysis revealed significant differences only between grades II and III and between grades I and III when HE was used (P Ͻ 0.01) and between grades I and III with panoptic stain (P Ͻ 0.05). The ellipticity factor and regularity factor did not reveal significant differences between histopathologic grades. The results indicate that nuclear morphometric analysis, in combination with the rapid and inexpensive cytopathology technique, can help in mast cell tumor grading, thus contributing to the establishment of a more precise prognosis and treatment.

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Morphometry in histopathology

Cited by 68 publications

References 61 publications

Nuclear Morphometry in Cytopathology: A Prognostic Indicator for Canine Cutaneous Mast Cell Tumors

Nuclear Morphometry in Cytopathology: A Prognostic Indicator for Canine Cutaneous Mast Cell Tumors

Mechanism of Insulin Uptake in Rat Alveolar Type II and Type I-Like Epithelial Cells

Morphometry of Canine Cutaneous Mast Cell Tumors

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