Morphometric study of the corpus cavernosum after anabolic androgenic steroid administration in pubertal and adult rats

Sena, Alessandro de Sousa Mendes de; Vargas, Rafael Areas; Souza, Diogo Benchimol de; Costa, Waldemar S.; Sampaio, Francisco J. B.

doi:10.1590/s0102-865020150070000005

Cited by 7 publications

(4 citation statements)

References 23 publications

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“… 34 35 The reduction in the amount of smooth muscle in the corpus cavernosum is commonly associated with ED in rodent models. This was observed by our group under multiple experimental conditions such as chronic stress, 19 anabolic steroid abuse, 36 and testosterone depletion. 33 This latter model (testosterone depletion) may be comparable in some ways to the animals used in this study.…”

Section: Discussionsupporting

confidence: 57%

The corpus cavernosum after treatment with dutasteride or finasteride: A histomorphometric study in a benign prostatic hyperplasia rodent model

Silva

et al. 2018

Asian J Androl

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Erectile dysfunction is a common side effect of finasteride and dutasteride treatments. The objective of this study was to investigate the structural changes in the penis using a benign prostatic hyperplasia (BPH) rodent model treated with dutasteride or finasteride. Sixty male rats were divided into the following groups: C, untreated control rats; C + D, control rats receiving dutasteride; C + F, control rats receiving finasteride; H, untreated spontaneously hypertensive rats (SHRs); H + D, SHRs treated with dutasteride; and H + F, SHRs treated with finasteride. Treatments were performed for 40 days, and penises were collected immediately thereafter. The organs were analyzed using histomorphometric methods to determine the cross-sectional penile area, as well as the surface density (Sv) of smooth muscle fibers, connective tissue, elastic system fibers, and sinusoidal spaces of the corpus cavernosum. The results were compared using a one-way ANOVA with Bonferroni's posttest. Groups C + D and C + F had a significantly smaller penile cross-sectional area, but more elastic system fiber Sv compared to Group C. Group C + D showed less smooth muscle Sv, and Group H showed more connective tissue but a smaller sinusoidal space Sv in the corpus cavernosum compared to Group C. Groups H + D and H + F had less smooth muscle Sv than Group H. Group H + D also had more connective tissue and elastic system fiber Sv than Group H. Both dutasteride and finasteride promoted penile modifications in the control rat penis, although this affect was greater in Group H animals. In this rodent model, dutasteride was the drug that most affected the corpus cavernosum.

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Section: Discussionsupporting

confidence: 57%

The corpus cavernosum after treatment with dutasteride or finasteride: A histomorphometric study in a benign prostatic hyperplasia rodent model

Silva

et al. 2018

Asian J Androl

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“…When treated groups reached 20 weeks of age, they underwent chronic use of nandrolone decanoate (Deca Durabolin 50 mg·mL –1 Organon, São Paulo, Brazil) at a dose of 10 mg·kg –1 of body weight by intramuscular injection, once a week during eight weeks. The control animals received intramuscular injection of vehicle (peanut oil) at the same amount during the same period in order to cause equal stress suffered by the treated animals 3 , 4 .…”

Section: Methodsmentioning

confidence: 99%

“…Nandrolone decanoate is an anabolic-androgenic steroid with several medical applications; however, it is indiscriminately used for fast increase of muscle mass 1 , 2 . Some studies have been performed in order to know its effects in different organs 3 , 4 .…”

Section: Introductionmentioning

confidence: 99%

Effects of nandrolone decanoate on femur morphology. Experimental study

et al. 2021

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Purpose To evaluate the immediate and late effects of nandrolone on femur morphology of rats. Methods Twenty-eight animals with 20 weeks of age were divided into four groups: C28, control animals that were euthanized eight weeks after the experiment started; C40, control animals euthanized 20 weeks after the experiment started; T28, treated animals receiving nandrolone during eight weeks and euthanized immediately after the treatment period; and T40, animals treated during eight weeks and euthanized 12 weeks after the end of the treatment. Treated animals received nandrolone decanoate during eight weeks and control groups received peanut oil by intramuscular injection. After euthanasia, femurs were removed, dissected, weighted and measured by digital pachymeter. Results The T40 group presented an increase on distal epiphysis diameter when compared to C40 group. There was no difference between treated and control groups in relation to body and femur absolute weight, relative weight and length of femur. There was also no difference in relation to diameter of proximal epiphysis and diameter of diaphysis among the groups. Conclusions Nandrolone decanoate does not produce significant effect on femur, exception on its distal extremity at late period. The effects of such drug may depend on the time after administration.

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“…Sirius red stained sections observed under polarization microscopy shows a birefringent image that is highly specific for collagen, and allows the differentiation of collagen types. This characteristic has been used to investigate the collagen in the prostate ( 24 , 25 ) as well as in other organs ( 26 , 27 ).…”

Section: Introductionmentioning

confidence: 99%

The prostate after castration and hormone replacement in a rat model: structural and ultrastructural analysis

et al. 2017

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Purpose:To evaluate if late hormonal replacement is able to recover the prostatic tissue modified by androgenic deprivation.Materials and Methods:24 rats were assigned into a Sham group; an androgen deficient group, submitted to bilateral orchiectomy (Orch); and a group submitted to bilateral orchiectomy followed by testosterone replacement therapy (Orch+T). After 60 days from surgery blood was collected for determination of testosterone levels and the ventral prostate was collected for quantitative and qualitative microscopic analysis. The acinar epithelium height, the number of mast cells per field, and the densities of collagen fibers and acinar lumen were analyzed by stereological methods under light microscopy. The muscle fibers and types of collagen fibers were qualitatively assessed by scanning electron microscopy and polarization microscopy.Results:Hormone depletion (in group Orch) and return to normal levels (in group Orch+T) were effective as verified by serum testosterone analysis. The androgen deprivation promoted several alterations in the prostate: the acinar epithelium height diminished from 16.58±0.47 to 11.48±0.29μm; the number of mast cells per field presented increased from 0.45±0.07 to 2.83±0.25; collagen fibers density increased from 5.83±0.92 to 24.70±1.56%; and acinar lumen density decreased from 36.78±2.14 to 16.47±1.31%. Smooth muscle was also increased in Orch animals, and type I collagen fibers became more predominant in these animals. With the exception of the densities of collagen fibers and acinar lumen, in animals receiving testosterone replacement therapy all parameters became statistically similar to Sham. Collagen fibers density became lower and acinar lumen density became higher in Orch+T animals, when compared to Sham. This is the first study to demonstrate a relation between mast cells and testosterone levels in the prostate. This cells have been implicated in prostatic cancer and benign hyperplasia, although its specific role is not understood.Conclusion:Testosterone deprivation promotes major changes in the prostate of rats. The hormonal replacement therapy was effective in reversing these alterations.

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Morphometric study of the corpus cavernosum after anabolic androgenic steroid administration in pubertal and adult rats

Abstract: The use of supra-physiological doses of AAS promotes structural changes in the rat penis, by altering the proportions of corpus cavernosum tissues, in both pubertal and adult treated animals.

Cited by 7 publications

References 23 publications

The corpus cavernosum after treatment with dutasteride or finasteride: A histomorphometric study in a benign prostatic hyperplasia rodent model

The corpus cavernosum after treatment with dutasteride or finasteride: A histomorphometric study in a benign prostatic hyperplasia rodent model

Effects of nandrolone decanoate on femur morphology. Experimental study

The prostate after castration and hormone replacement in a rat model: structural and ultrastructural analysis

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