Morphometric study of purkinje cells in the dog cerebellar cortex

Avrushchenko, M. Sh.

doi:10.1007/bf00829559

Cited by 6 publications

(8 citation statements)

References 5 publications

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“…10 Tissue screening was performed by means of an optical microscope with a 40x objective coupled with a video capture monitor and a counting system. Visual neuron quantification was expressed in numbers per area in a sequential pattern in accordance with Avrushchenko, Michon et al, Rabinowicz et al, and Esteves et al [10][11][12][13] Five serial sections from the frontal lobe region were selected and ten different fields were screened to produce a mean for statistical analysis. Photomicrographs of the best fields were taken.…”

Section: Methodsmentioning

confidence: 99%

Effects of fluoxetine and imipramine in rat fetuses treated during a critical gestational period: a macro and microscopic study

Swerts

Costa

Esteves

et al. 2009

Rev. Bras. Psiquiatr.

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OBJECTIVE: To evaluate morphological alterations in rat fetuses treated with fluoxetine and imipramine during the "critical" period of gestation. METHOD: Fifteen female rats were separated into three groups (n = 5) and treated with 10 mg/kg/day of test substances on the ninth, tenth and eleventh day of pregnancy: G1, fluoxetine; G2, imipramine hydrochloride; G3 (control), saline. On day 21, cesarean sections were performed to release the fetuses, whose bodies were weighed and macroscopically analyzed. The placenta was also weighed. The fetuses were then fixed and their encephala removed and weighed. Sections of the frontal lobe were taken for histological neuron counting. RESULTS: G1 and G2 showed the highest fetal body weight. Placental weight showed statistical differences (p < 0.01): G1 weighed more than G2 and G3. Otherwise, G2 exhibited the highest encephalon weight, statistically differing from G3 (control) and fluoxetine-treated G1 (p < 0.01). However, G1 did not statistically (p > 0.01) differ from the control group. G3 showed the highest number of neurons per area when compared to G1 and G2 (p < 0.01). CONCLUSION: The use of antidepressants in rats caused an increase in fetal weight and a decrease in the number of fetal frontal lobe neurons, thus suggesting that the use of antidepressants by pregnant women can induce depression in fetuses due to alterations in their neural development.

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Section: Methodsmentioning

confidence: 99%

Effects of fluoxetine and imipramine in rat fetuses treated during a critical gestational period: a macro and microscopic study

Swerts

Costa

Esteves

et al. 2009

Rev. Bras. Psiquiatr.

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“…Para a análise quantitativa, foram selecionados cinco cortes por região, seguindo-se então a mensuração manual com auxílio de um retículo desenhado e fixado no monitor do computador para quantificação de pontos inseridos somente dentro dos quadrantes, excluindo pontos nas linhas do retículo. Em cada corte foram contados cinco campos distintos, obtendo-se deles uma média; das cinco médias obteve-se uma média final para análise estatística 9,13,14 .…”

Section: Procedimentounclassified

Quantificação de Corpos de Neurônios em Camundongos Submetidos ao Uso de Esteróides Anabolizantes

et al. 2001

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Rev Neurocienc 2012;20(1):68-72 original 68 RESUMO Objetivo. Verificar possíveis alterações quantitativas de corpos de neurônios, no córtex cerebral, causadas pelo uso de esteróides anabolizantes. Método. Foram utilizados trinta camundongos divididos em 3 grupos (grupo 1 -Deca Durabolin®; grupo 2-Potenay®; grupo 3-Salina) tratados uma vez na semana e submetidos à natação três vezes na semana, tendo o tratamento a duração total de um mês. Após a eutanásia os encéfalos foram retirados e foram preparados segundo técnica histológica convencional e corados com violeta cresil, através da análise morfométrica foram buscados os dados comparativos. Resultados. Os resultados obtidos revelaram que houve uma diminuição significativa do número de corpos de neurônios nos animais tratados (P<0,01) quando comparados com o grupo controle, porém não foi significativo (P>0,05) quando comparados os dois sexos. Os animais do grupo Potenay® apresentaram 23,36 corpos de neurônios enquanto que os animais do grupo Deca-durabolin® apresentaram 23,40 corpos de neurônios, os valores médios de corpos de neurônios foram iguais e inferiores aos observados para o grupo controle que foi de 31,70. Conclusão. Concluímos que a densidade de corpos de neurônios no córtex cerebral mostra uma diminuição significativa nos camundongos submetidos ao tratamento com esteróides anabolizantes em relação ao grupo controle. ABSTRACTObjective. Investigate quantitative changes in cerebral cortex neurons bodies caused by the use of anabolic steroids. Method. Thirty mice were used, which received treatment with steroids (group 1-Deca Durabolin®; group 2-Potenay®; group 3-Saline) once a week and submitted to swimming three times a week, taking total treatment duration one month. After euthanasia, the brains were removed, fixed and histological processed and cresyl violet was used for staining. The slices were analyzed by light microscope and software to count the neurons bodies. Results. The results shows that there was a significant decrease of neurons bodies number in treated groups (P<0.01) when compared with the control group, but was not significant (P>0.05) when both genders were compared. In Potenay ® group, the animals showed 23.36 neurons bodies while the animals in Deca-durabolin® group had 23.40. The average values of neuron bodies were equal and lower than of control group, which was 31.70. Conclusion. The neuronal bodies density in cerebral cortex decreased significantly in mice undergoing treatment with steroids in relation to control group. Original Recebido em: 15/03/11 Aceito em: 09/08/11 Conflito de interesses: não Suporte financeiro: Fapemig. Trabalho realizado no Departamento de Anatomia da Universidade Federal de Alfenas, Alfenas-MG, Brasil. 1.Biomédico graduando pela Unifal-MG, Alfenas-MG, Brasil. 2.Biólogo graduando pela Unifal-MG, Alfenas-MG, Brasil. 3.Zootecnista, Doutor e Docente da Unifal-MG, Alfenas-MG, Brasil. 4.Cirurgião dentista, Doutor Docente da Unifal-MG, Alfenas-MG, Brasil. 5.Médico, Doutor Docente da Unifal-MG, Alfenas-MG, Brasil....

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“…The cerebellum consists of 2 main components, the cerebellar cortex and the medulla. There are several cellular components in the cerebellar cortex; the most distinguished morphologically are Purkinje cells (PCs), granule, Golgi and basket cells 5 . This complex neural network gives rise to a massive signal-processing capability that plays a role in motor learning 6 .…”

Section: Introductionmentioning

confidence: 99%

An updated investigation on the dromedary camel cerebellum (Camelus dromedarius) with special insight into the distribution of calcium-binding proteins

Attaai

Noreldin

Abdel-Maksoud

et al. 2020

Sci Rep

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Studying the cerebella of different animals is important to expand the knowledge about the cerebellum. Studying the camel cerebellum was neglected even though the recent research in the middle east and Asia. Therefore, the present study was designed to achieve a detailed description of the morphology and the cellular organization of the camel cerebellum. Because of the high importance of the calcium ions as a necessary moderator the current work also aimed to investigate the distribution of calcium binding proteins (CaBP) such as calbindin D-28K (CB), parvalbumin (PV) and calretinin (CR) in different cerebellar cells including the non-traditional neurons. The architecture of camel cerebellum, as different mammals, consists of the medulla and three layered-cortex. According to our observation the cells in the granular layer were not crowded and many spaces were observed. CB expression was the highest by Purkinje cells including their dendritic arborization. In addition to its expression by the inhibitory interneurons (basket, stellate and Golgi neurons), it is also expressed by the excitatory granule cells. PV was expressed by Purkinje cells, including their primary arborization, and by the molecular layer cells. CR immunoreactivity (-ir) was obvious in almost all cell layers with varying degrees, however a weak or any expression by the Purkinje cells. The molecular layer cells and the Golgi and the non traditional large neurons of the granular layer showed the strongest CR-ir. Granule neurons showed moderate immunoreactivity for CB and CR. In conclusion, the results of the current study achieved a complete map for the neurochemical organization of CaBP expression and distribution by different cells in the camel cerebellum.

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Morphometric study of purkinje cells in the dog cerebellar cortex

Cited by 6 publications

References 5 publications

Effects of fluoxetine and imipramine in rat fetuses treated during a critical gestational period: a macro and microscopic study

Effects of fluoxetine and imipramine in rat fetuses treated during a critical gestational period: a macro and microscopic study

Quantificação de Corpos de Neurônios em Camundongos Submetidos ao Uso de Esteróides Anabolizantes

An updated investigation on the dromedary camel cerebellum (Camelus dromedarius) with special insight into the distribution of calcium-binding proteins

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