Morphometric pattern of somatic chromosomes in three Romanian seabuckthorn genotypes

Abstract

The somatic chromosomes and karyotype traits of three Romanian genotypes (coded as CB-1, S-16, L-4) of Hippophae rhamnoides L. have been studied. Diploid chromosome number is 2n=24. The karyomorphological data show that the complements have small chromosomes (1.33 ± 0.014 -2.80±0.01 µm, for CB-1 genotype; 1.13 ± 0.029 -2.90 ± 0.029 µm, for S-16; 1.15 ± 0.099 -2.88 ± 0.024 µm, for L-4 genotype) and the length of haploid complements is 23.97 µm, for CB-1, 22.20 µm, for S-16, 21.73 µm, for L-4. In CB-1 and S-16 male genotypes, the putative Y-chromosome has larger sizes than X-chromosome and it is of metacentric type (CI = 45.77, r = 1.18, for CB-1; CI = 46.22, r = 1.15, for S-16). The L-4 female karyotype is constituted by twelve pairs of morphologically similar chromosomes. The karyotype formulae are 2n = 24 = 13m + 11sm (putative male: 22 + XY), for CB-1 genotype, 2n = 24 = 18m + 6sm (putative male: 22 + XY), for S-16 genotype, respectively 2n = 24 = 22m + 2sm (L-4 genotype, considered as female: 22 + XX). The small size of chromosomes (< 3µm), the presence of only two morphometric chromosome types and preponderance of metacentrics confer a relatively high degree of symmetry to the studied karyotypes. Our results show a low intraspecifi c karyotype variation.

Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae)

“…An X/Y gender determination system is indicated by the findings of a heteromorphic chromosome pairs in male plants (Schapov 1977;Elena et al 2011). The total chromosome length of the Y-heterosome exceeds that of the X-chromosome (Elena et al 2011).…”

“…An X/Y gender determination system is indicated by the findings of a heteromorphic chromosome pairs in male plants (Schapov 1977;Elena et al 2011). The total chromosome length of the Y-heterosome exceeds that of the X-chromosome (Elena et al 2011). Presence of female-linked DNA-based markers in large percentage of the population, as shown in this study, confirms that gender in seabuckthorn is most likely genetically determined.…”

Mining of microsatellites using next generation sequencing of seabuckthorn (Hippophae rhamnoides L.) transcriptome

“…It is a hardy, deciduous shrub, with significant medicinal and ecological importance. All the species of Hippophae are diploid with 2n =24 (Elena et al 2011). Seabuckthorn berries are rich in vitamins, fatty acids, free amino acids, flavanoids, tocopherol and other secondary metabolites (Dhyani et al 2007).…”

Sea-buckthorn: a species with a variety of uses, especially in land reclamation

“…A shortcoming of sea-buckthorn is represented by the lack of an efficient method able to differentiate the female and male individuals in order to assure the favorable sex ratio if, for example, someone plans to install a crop (Truta et al 2011). In order to resolve this problem, vegetative propagation from mature plants with known sex should be applied.…”