Morphometric investigations of the colon mucosa in chronicTrypanosoma cruzi infected rats

Fernandes, Maria Inez Machado; Zucoloto, Sérgio; Collares, Edgard Ferro; Filho, F. Ferriolli

doi:10.1007/bf02899536

Cited by 16 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In one of these studies, a denervation was detected of up to 75% in the colon of mice with Chagas' disease that had dilatation and a denervation of up to 87% in those that did not have dilatation [12]. Experimental studies in which morphometric evaluations were made did not detect megacolon in rats, despite there being denervation [6,14].…”

Section: Discussionmentioning

confidence: 97%

Neuron count reevaluation in the myenteric plexus of chagasic megacolon after morphometric neuron analysis

et al. 2001

View full text Add to dashboard Cite

This study was made with the objective of reevaluating the colon denervation in chronic Chagas' disease. The diameters of neuron perikaryons of the myenteric plexus were measured on paraffin sections in a ring from the sigmoid in Chagas' disease patients, 17 with and 10 without megacolon and in 10 non-chagasic controls. All neurons were counted in ten en-echelon sections. Neuron hypertrophy only occurred in the group with megacolon, and the average increase in diameter was 69.3%. This could generate an error factor in the neuron count by increasing the probability of neurons being seen in a greater number of histological sections. The original result of the neuron count gave medians of 1264, 1961, and 2665 in the groups of chagasic patients with megacolon, without megacolon, and in the control, respectively. The denervation was greater than 55% in only seven megacolon cases (41.2%). After applying a correction factor, the median in the group with megacolon was 746, and the denervation was greater than 55% in 13 cases (76.5%). This occurrence demonstrates the need to apply a correction factor when the neuron count in chagasic megacolon is being evaluated and in the other pathologies where neuron hypertrophy may be found.

show abstract

Section: Discussionmentioning

confidence: 97%

Neuron count reevaluation in the myenteric plexus of chagasic megacolon after morphometric neuron analysis

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Little is known about the epithelium in denervated colon. Fernandes et al [5] have shown myenteric plexus denervation in colon from rats infected with Trypanosoma cruzi and hyperplasia of the epithelium. Similar data were observed in the descending, colon with an increase of the serotonin level, a decrease of the acetylcholine level and no change in the catecholamine level using BAC as myenteric plexus denervation [26].…”

Section: Discussionmentioning

confidence: 98%

The relationship between myenteric neuronal denervation, smooth muscle thickening and epithelial cell proliferation in the rat colon

et al. 1997

View full text Add to dashboard Cite

The effects of myenteric neuronal denervation on smooth muscle thickening and epithelial cell proliferation were studied in the descending colon of rats treated by serosal application of 2 mM benzalkonium chloride (BAC) for 30 min. Control animals were treated with saline (0.9% NaCl). The animals were divided into six groups of 13 animals each and killed 10, 45 and 120 days after BAC treatment. A significant reduction in neuron number was observed in the myenteric plexus of animals treated with BAC, as well as smooth muscle thickening and an increase in crypt cell population, crypt cell production per crypt and a decrease in cell cycle time. These findings permit us to conclude that a relationship may exist between the increase of epithelial cell proliferation, smooth muscle thickening and myenteric neuron denervation in the descending colon caused by BAC, the latter probably playing an important role in the integration of the other two.

show abstract

“…Alterations in the absorption of carbohydrates may occur as a consequence of parasympathetic denervation of the small intestine 24 . Studies with rats experimentally infected by T. cruzi showed that loss of neurons from the myoenteric plexus may cause disequilibrium in the functioning of the remaining neurons and in the production of some neurotransmitters 25 . It has also been observed that women with the chronic cardiac form of Chagas' disease have diabetes mellitus and disturbances in the regulation of glycemic level at a higher frequency than normal controls, probably due to reduced activity of the parasympathetic system 26 .…”

Section: Discussionmentioning

confidence: 99%

Morphologic and morphometric evaluation of pancreatic islets in chronic Chagas' disease

Saldanha

Santos²,

Reis³

et al. 2001

Rev. Hosp. Clin.

View full text Add to dashboard Cite

Purpose: Hyperglycemia and abnormal glucose tolerance tests observed in some patients with chronic Chagas' disease suggest the possibility of morphological changes in pancreatic islets and/or denervation. The purpose of this study was to describe the morphology and morphometry of pancreatic islets in chronic Chagas' disease.Methods: Morphologic and computerized morphometric studies were performed in fragments of the head, body, and tail regions of the pancreas obtained at necropsies of 8 normal controls and 17 patients with chronic Chagas' disease: 8 with the digestive form (Megas) and 9 with the congestive heart failure form.Results: The Megas group had a larger (p < 0.05) pancreatic islet area in the tail of the pancreas (10649.3 ± 4408.8 µm 2 ) than the normal control (9481.8 ± 3242.4 µm 2 ) and congestive heart failure (9475.1 ± 2104.9 µm 2 ) groups; likewise, the density of the pancreatic islets (PI) was greater (1.2 ± 0.7 vs. 0.9 ± 0.6 vs. 1.9 ± 1.0 PI/mm 2 , respectively). In the tail region of the pancreas of patients with the Megas form, there was a significant and positive correlation (r = +0.73) between the area and density of pancreatic islets. Discrete fibrosis and leukocytic infiltrates were found in pancreatic ganglia and pancreatic islets of the patients with Chagas' disease. Trypanosoma cruzi nests were not observed in the examined sections. Individuals with the Megas form of Chagas' disease showed increased area and density of pancreatic islets in the tail of the pancreas.Conclusion: The observed morphometric and morphologic alterations are consistent with functional changes in the pancreas, including glycemia and insulin disturbances.

show abstract

Morphometric investigations of the colon mucosa in chronicTrypanosoma cruzi infected rats

Cited by 16 publications

References 15 publications

Neuron count reevaluation in the myenteric plexus of chagasic megacolon after morphometric neuron analysis

Neuron count reevaluation in the myenteric plexus of chagasic megacolon after morphometric neuron analysis

The relationship between myenteric neuronal denervation, smooth muscle thickening and epithelial cell proliferation in the rat colon

Morphologic and morphometric evaluation of pancreatic islets in chronic Chagas' disease

Contact Info

Product

Resources

About