Morphometric discrimination between females of two isomorphic sand fly species, Phlebotomus caucasicus and Phlebotomus mongolensis (Diptera: Phlebotominae) in endemic and non-endemic foci of zoonotic cutaneous leishmaniasis in Iran

Absavaran, Azad; Mohebali, Mehdi; Moin-Vaziri, Vahideh; Zahraei-Ramazani, Alireza; Akhavan, Amir Ahmad; Mozaffarian, Fariba; Rafizadeh, Sayena; Rassi, Yavar

doi:10.4103/1995-7645.257115

Cited by 4 publications

(5 citation statements)

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“…These results show a tendency of morphological differentiation of P. davisi populations, mainly of specimens from the southeast region, raising the need to evaluate a more comprehensive sampling, and other taxonomic characters of these taxa (e.g., Godoy et al, 2018). Wing GMM of sand flies is able to delineate some genera (Godoy et al, 2014) and closely related species (Absavaran et al, 2019), and even help in taxa description (Shimabukuro et al, 2016). Furthermore, species that are widely distributed have structured populations discriminated by GMM analyses (Aytekin et al, 2007; Dujardin et al, 2003; Mikery et al, 2019), which may indicate changes in the biology of these insects since, for example, high‐altitude specimens might have larger wings than low‐altitude ones (Dujardin et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…However, this species is found in great abundance in endemic localities for leishmaniasis, with positive testing for Leishmania DNA ( Ávila et al, 2018;Leão et al, 2020;Pereira Júnior et al, 2015Silva et al, 2014;Silva et al, 2021;Valdivia et al, 2012) and flagellates (Gil et al, 2003;Souza et al, 2010). In addition, specific localities incriminate this species as a putative vector of L. naiffi and L. braziliensis due to its great abundance and anthropophilic status (Gil et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…However, the discovery of cryptic species must be validated with additional information (Struck et al, 2018). In the case of sand flies, traditional morphometry and geometric morphometry (GMM) are used to discriminate closely related species (Absavaran et al, 2019) and structured populations of the same taxon (Mikery et al, 2019). Furthermore, supplementary morphological characteristics have already been used for the validation of previously established species complexes by DNA Barcoding (Yogeswari & Srinivasan, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Molecular and morphometric study of Brazilian populations of Psychodopygus davisi

Rodrigues,

da Silva Costa,

Godoy

et al. 2023

Medical Vet Entomology

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In this study, we analysed the molecular and morphometric differences of several populations of the putative sand fly vector Psychodopygus davisi (Root, 1934) (Diptera, Psychodidae, Phlebotominae) in Brazil. We amplified the 658 base pair fragments of the DNA barcoding region—cytochrome c oxidase subunit 1 (COI) gene—for 57 specimens of P. davisi and three specimens of Psychodopygus claustrei (Abonnenc, Léger & Fauran, 1979). We merged our data with public sequences of the same species available from GenBank. Then, the combined dataset—87 sequences and 20 localities—was analysed using population structure analysis and different species delimitation approaches. Geometric morphometry of wings was performed for 155 specimens of P. davisi populations from the North, Midwest and Southeast Brazilian regions, analysing the differences in centroid sizes and canonical variates. Molecular analysis indicated high intraspecific genetic distance values for P. davisi (maximum p distance = 5.52%). All algorithms identified P. davisi and P. claustrei as distinct molecular taxonomic units, despite the low interspecific distance (p distance to the nearest neighbour = 4.79%). P. davisi sequences were split into four genetic clusters by population structure analysis and at least five genetic lineages using intermediate scenarios of the species delimitation algorithms. The species validation analysis of BPP strongly supported the five‐species model in our dataset. We found high genetic diversity in this taxon, which is in agreement with its wide geographic distribution in Brazil. Furthermore, the wing analysis showed that specimens from the Southeast Region of Brazil are different from those in the North and the Midwest. The evolutionary patterns of P. davisi populations in Brazil suggest the presence of candidate species, which need to be validated in future studies using a more comprehensive approach with both genomic data and morphological characters.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular and morphometric study of Brazilian populations of Psychodopygus davisi

Rodrigues,

da Silva Costa,

Godoy

et al. 2023

Medical Vet Entomology

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“…At first, for removing castor oil on specimen’s body surface, collected specimens were washed twice in 1% detergent and sterile distilled water and then was dissected in a drop of sterile normal saline by sterilized forceps. The head and the last two abdominal segments were cut off and slide mounted in Puris' medium for species identification and identified after 24–72 hours using valid identification keys ( 13 , 28 – 30 ). The remaining body (abdomen, wings, and legs) were preserved in 1.5ml sterile micro-tubes containing 96% ethanol for DNA extraction and detection of Leishmania parasite.…”

Section: Methodsmentioning

confidence: 99%

“…mongolensis not only participate in the transmission cycle of ZCL among reservoir hosts but also play an important role as secondary vectors in the transmission of leishmaniasis to humans ( 8 ). The females of these species have similar taxonomic characteristics and are isomorphic but based on recent study, morphometric analysis and morphological characters have been used for discrimination of these closely related species ( 13 ). Natural promastigote infection was isolated from Ph.…”

Section: Introductionmentioning

confidence: 99%

First Report of Natural Infection of Phlebotomus mongolensis to Leishmania major and Leishmania turanica in the Endemic Foci of Zoonotic Cutaneous Leishmaniasis in Iran

Absavaran¹,

Mohebali²,

Moin-Vaziri³

et al. 2023

JAD

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Background: The primary aim of this study is to determine infection to Leishmania parasites in the wild population of Phlebotomus caucasicus and Phlebotomus mongolensis using molecular methods in some important zoonotic cutaneous leishmaniasis foci in Iran. Methods: Sand flies were collected from active colonies of rodent burrows from 16 trapping sites using sticky trap paper. In order to detect and identify of Leishmania parasites in females Ph. caucasicus and Ph. mongolensis, the Nested–PCR amplification of ITS2-rDNA region was performed to generate amplicon with 245bp for Leishmania major, 206bp for L. gerbilli and 141bp for L. turanica. Results: In the current study we found DNA of different gerbil parasites such as L. major and L. turanica, and mixed infection of L. major/L. turanica in Ph. caucasicus and Ph. mongolensis. It should be noted that, in Iran, natural infection with Leishmania parasites is recorded for the first time in this study in Ph. mongolensis. Conclusion: Both species of Ph. caucasicus and Ph. mongolensis not only may participate in the ZCL transmission cycle between reservoir hosts, but also results of this study support the role of these species as secondary vectors in the transmission of leishmaniasis to humans.

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Analysis of morphological variations of three Adlerius (Diptera: Psychodidae) species collected in two cutaneous leishmaniasis endemic foci of Turkey

Kavur

Arıkan

Töz

et al. 2021

Int J Trop Insect Sci

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Morphometric discrimination between females of two isomorphic sand fly species, Phlebotomus caucasicus and Phlebotomus mongolensis (Diptera: Phlebotominae) in endemic and non-endemic foci of zoonotic cutaneous leishmaniasis in Iran

Cited by 4 publications

References 1 publication

Molecular and morphometric study of Brazilian populations of Psychodopygus davisi

Molecular and morphometric study of Brazilian populations of Psychodopygus davisi

First Report of Natural Infection of Phlebotomus mongolensis to Leishmania major and Leishmania turanica in the Endemic Foci of Zoonotic Cutaneous Leishmaniasis in Iran

Analysis of morphological variations of three Adlerius (Diptera: Psychodidae) species collected in two cutaneous leishmaniasis endemic foci of Turkey

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