Morphometric comparisons of the scanning electron micrographs of the eggs of Anopheles (Nyssorhynchus) darlingi Root (Diptera: Culicidae)

Almeida, Fabio de; Suesdek, Lincoln; Motoki, Maysa Tiemi; Bergo, Eduardo Sterlino; Sallum, Maria Anice Mureb

doi:10.1016/j.actatropica.2014.07.010

Acta Tropica

2014

DOI: 10.1016/j.actatropica.2014.07.010

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Morphometric comparisons of the scanning electron micrographs of the eggs of Anopheles (Nyssorhynchus) darlingi Root (Diptera: Culicidae)

Fabio de Almeida

Lincoln Suesdek

Maysa Tiemi Motoki

et al.

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Cited by 4 publications

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“…darlingi, associated with differentiation in behavioural, morphological, and genetic traits, (4,32,33) raised the hypothesis that this species would comprise a group of cryptic species. For instance, there are reports describing variations in the egg (exochorion) morphology, (34) wings morphometry, (35,36,37) hematophagy activity behaviour, and lifespan. (38,39) Besides, polymorphism in the banding patterns of polytene chromosomes also suggested cytogenetic variations.…”

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Molecular diversity of genes related to biological rhythms (period and timeless) and insecticide resistance (Na V and ace-1) in Anopheles darlingi

Loureiro

Araki

Bruno

et al. 2023

Mem. Inst. Oswaldo Cruz

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BACKGROUND Malaria is a public health concern in the Amazonian Region, where Anopheles darlingi is the main vector of Plasmodium spp. Several studies hypothesised the existence of cryptic species in An. darlingi , considering variations in behaviour, morphological and genetic aspects. Determining their overall genetic background for vector competence, insecticide resistance, and other elements is essential to better guide strategies for malaria control. OBJECTIVES This study aimed to evaluate the molecular diversity in genes related to behaviour and insecticide resistance, estimating genetic differentiation in An. darlingi populations from Amazonian localities in Brazil and Pacific Colombian region. METHODS We amplified, cloned and sequenced fragments of genes related to behaviour: timeless ( tim ) and period ( per ), and to insecticide resistance: voltage-gated sodium channel ( Na V ) and acetylcholinesterase ( ace - 1 ) from 516 An. darlingi DNA samples from Manaus, Unini River, Jaú River and Porto Velho - Brazil, and Chocó - Colombia. We discriminated single nucleotide polymorphisms (SNPs), determined haplotypes and evaluate the phylogenetic relationship among the populations. FINDINGS The genes per , tim and ace-1 were more polymorphic than Na V . The classical kdr and ace-1 R mutations were not observed. Phylogenetic analyses suggested a significant differentiation between An. darlingi populations from Brazil and Colombia, except for the Na V gene. There was a geographic differentiation within Brazilian populations considering per and ace-1 . CONCLUSIONS Our results add genetic data to the discussion about polymorphisms at population levels in An. darlingi . The search for insecticide resistance-related mechanisms should be extended to more populations, especially from localities with a vector control failure scenario.

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confidence: 99%

Molecular diversity of genes related to biological rhythms (period and timeless) and insecticide resistance (Na V and ace-1) in Anopheles darlingi

Loureiro

Araki

Bruno

et al. 2023

Mem. Inst. Oswaldo Cruz

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Anopheles goeldii Rozeboom & Gabaldón (Diptera, Culicidae): a species of the Nuneztovari Complex of Anopheles Meigen

Sant'Ana

Bergo

Sallum

2015

Revista Brasileira de Entomologia

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Scanning Electron Microscopy and Geometric Contour Morphometry for Identifying Eggs of Three Amazonian Species of Mansonia (Diptera: Culicidae)

Ferreira

Simões

Ferreira-Keppler

et al. 2019

Journal of Medical Entomology

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The eggs of three Amazonian species of the genus Mansonia (Diptera: Culicidae) were analyzed using morphological and morphometric characters. Eggs of Mansonia humeralis Dyar & Knab, 1916 were morphologically different from those of Mansonia titillans (Walker, 1848) and Mansonia amazonensis (Theobald, 1901), which were more similar to each other according to linear and geometric morphometry. A principal component analysis generated from elliptic Fourier contour data (PC1—92.6% and PC2—2.61%) indicated that Ma. amazonensis and Ma. titillans are more similar to each other than either is to Ma. humeralis. Discriminant multivariate analysis was highly accurate with only four classification errors and a 90% accuracy rate. The results indicate that the three Amazonian species can be precisely distinguished in the egg stage and that geometric morphometry based on elliptic Fourier contours is a promising method for distinguishing eggs of species of Mansonia. An identification key based on egg morphology is provided to distinguish the four Neotropical species.

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Morphometric comparisons of the scanning electron micrographs of the eggs of Anopheles (Nyssorhynchus) darlingi Root (Diptera: Culicidae)

Cited by 4 publications

References 28 publications

Molecular diversity of genes related to biological rhythms (period and timeless) and insecticide resistance (Na V and ace-1) in Anopheles darlingi

Molecular diversity of genes related to biological rhythms (period and timeless) and insecticide resistance (Na V and ace-1) in Anopheles darlingi

Anopheles goeldii Rozeboom & Gabaldón (Diptera, Culicidae): a species of the Nuneztovari Complex of Anopheles Meigen

Scanning Electron Microscopy and Geometric Contour Morphometry for Identifying Eggs of Three Amazonian Species of Mansonia (Diptera: Culicidae)

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