Morphometric characterization of left ventricular myocardial cells of male rats during postnatal development

David, Heinz; Meyer, R.; Marx, I; Guski, H; Wenzelides, K

doi:10.1016/0022-2828(79)90377-8

Cited by 24 publications

(12 citation statements)

References 10 publications

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“…Even though CCC significantly diminishes intracellular acidification and loss of ATP in the ischemic neonatal heart, our results as well as those measuring mechanical function [9,19] sug gest that CCC is not as effective in the neonate as in the adult. Again, these results are consis tent with models [38,39] and data [6,7,30,[34][35][36][37] discussed above that led us to postu late that the neonatal heart is disposed and required to rely on anaerobic metabolism ear lier than the adult when oxygen supply is reduced. Nevertheless, throughout 2 h of re perfusion hearts exposed to CCC maintain ATP levels significantly greater than those not exposed to CCC.…”

Section: Discussionsupporting

confidence: 70%

“…In our experiments, the relative lack of PCr in the neonate hearts means the adult hearts would have a greater source of high-energy phosphate to maintain ATP during ischemia without relying on anaerobic metabolism and its associated net proton production. Factors which may contribute to the relatively low PCr observed in neonatal rabbit hearts have been well documented [30,31,[34][35][36][37] and would tend to diminish aerobic ATP (and PCr) production capacity in the neonatal heart. The neonatal heart's greater efficiency in pressure development with oxygen con sumption [6,7] suggests a relatively greater basal reliance on anaerobic glycolysis.…”

Section: Discussionmentioning

confidence: 99%

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Are Age-Related Differences in Response to Myocardial Ischemia and Cardioplegia pH Dependent?

et al. 1994

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Phosphorus-31 nuclear magnetic resonance and left ventricular pressure development (dP/dt) were used to test the hypothesis that age-related differences in myocardial functional recovery after ischemia and cold crystalloid cardioplegia (CCC) are the result of an inverse relationship between recovery and the decrease in intracellular pH (pH_i) during ischemia. Neonatal (3-8 days) and adult rabbit hearts were Langendorff perfused using two protocols: (1) control – 30min perfusion, 30 min global ischemia, 2 h reperfusion; (2) CCC – the same except ischemia was initiated after a 4-min infusion of cold hyperkalemic solution. Analysis of variance and the Tukey test showed the following significant differences between the protocols (p < 0.05). CCC decreased inorganic phosphate (Pi) during ischemia in both age groups, but more in adult hearts, and decreased Pi during reperfusion in adult hearts. CCC increased pH_i during ischemia and ATP during ischemia and reperfusion in both age groups but more in adult hearts. CCC increased dP/dt during reperfusion only in adult hearts. The results are consistent with the hypothesis.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Are Age-Related Differences in Response to Myocardial Ischemia and Cardioplegia pH Dependent?

et al. 1994

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“…At least 20% of cardiomyocyte volume is composed of mitochondria in human and 32% in mouse [46] [47], enabling efficient energy production via oxidative phosphorylation. At basal metabolic rates, more than 95% of energy that sustains cardiac function and viability is derived from aerobic metabolism [28].…”

Section: Discussionmentioning

confidence: 99%

Redox proteomic identification of HNE-bound mitochondrial proteins in cardiac tissues reveals a systemic effect on energy metabolism after doxorubicin treatment

Zhao

Miriyala

Liu

et al. 2014

Free Radical Biology and Medicine

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Doxorubicin (DOX), one of the most effective anticancer drugs, is known to generate progressive cardiac damage, which is due, in part, to DOX-induced reactive oxygen species (ROS). The elevated ROS often induce oxidative protein modifications that result in alteration of protein functions. This study demonstrates that the level of proteins adducted by 4-hydroxy-2-nonenal (HNE), a lipid peroxidation product, is significantly increased in mouse heart mitochondria following DOX treatment. A redox proteomics method involving 2D electrophoresis followed by mass spectrometry and investigation of protein data bases identified several HNE-modified mitochondria proteins, which were verified by HNE-specific immunoprecipitation in cardiac mitochondria from the DOX-treated mice. The majority of the identified proteins are related to mitochondrial energy metabolism. These include proteins in the citric acid cycle (TCA) and electron transport chain (ETC). The enzymatic activities of the HNE-adducted proteins were significantly reduced in DOX-treated mice. Consistent with the decline in the function of the HNE adducted proteins, the respiratory function of cardiac mitochondria as determined by oxygen consumption rate (OCR) was also significantly reduced after DOX treatment. The treatment with Mn(III) meso-tetrakis(N-n-butoxyethylpyridinium-2-yl)porphyrin, MnP, an SOD mimic, averted the doxorubicin-induced mitochondrial dysfunctions as well as the HNE protein adductions. Together, the results demonstrate that free radical-mediated alteration of energy metabolism is an important mechanism mediating DOX-induced cardiac injury suggesting that metabolic intervention may represent a novel approach to preventing cardiac injury after chemotherapy.

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“…There is no general agreement among various authors regarding the relative volumes of the subcellular components even in the same species in normal animals. The rat, because of its easy availability as an animal model, has been used by several Bossen et al, 1978Tate and Herbener, 1976Anversa et al, 1976Anversa et al, 1978Datta and Silver, 1975David et al, 1979Hatt et al, 1978Hirakow and Tadeshi, 1975Imamma, 1978Kamamura et al, 1976Laguens, 1971Laguens and Gomez-Dunn, 1967Legato, 1976Lund and Tomanek, 1978Malletal., 1977Mall et al, 1980McCallister et al , 1979Pageetal., 1971Page et al, 1972Page et al, 1974Page and McCallister, 1973Pager, 1971 Reith and Fuchs, 1973Tomanek et al, 1979Colgan et al, 1978Lazarus et al, 1976Anversa et al, 1971Goldstein et al, 1974Malletal, 1977Shendan et al, 1977Legato, 1976Legato, 1979McCallister et al, 1976Vitah-Mazza and Anversa, 1972 All values axe mean ± HEM unless otherwise indicated. See glossary for abbreviations. '…”

Section: Discussionmentioning

confidence: 99%

Myocardial morphometric characteristics in swine.

Singh¹,

White²,

Bloor³

1981

Circulation Research

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SUMMARYWe have quantified the ultrastructure of myocardial cells in tissue samples obtained from six adult normal Yucatan swine, tissue samples were obtained from 20 different sites in the left ventricle, right ventricle, and interventricular septum. The samples were processed for electron microscopy and quantitative morphometric analysis. The percent relative volumes of myoflbrils, mitochondria, transverse tubular system, sarcoplasmic reticulum, and clear intracellular space were determined using stereologic&l morphometric methods. Overall, the data were distributed homogenously among the 20 individual sites. However, when certain eubcellular components were examined regionally, some differences appeared. The mitochondrial:myofibril volume ratios were significantly increased in the left ventricle and interventricular septum compared to the right ventricle. They also were greater in the endocardial region compared to the epicardium, which is consistent with higher metabolic activity of these zones. Similarly, the sarcoplasmic reticulum :myofibril ratio was greater in the endocardial region vs. the epicardial region and the basal layer compared to the mid-layer, suggesting the greater need for provision of calcium ions in these particular cones. Since the swine is a convenient large animal fur physiological studies, these results indicate that swine can also be used for quantitative morphometric measurements of myocardial changes in normal and pathologic hearts, and for the study of regional changes in various layers and regions of heart walls. Circ Res 49: 434-441, 1981 MANY experimental models of cardiac hypertrophy have been used to describe the different functional, structural, and biochemical alterations that occur during the development of hypertrophy and failure in the heart. We nave used swine as a model of cardiac hypertrophy (White et al., 1979) since its response to exercise stress is similar to that in humans. Also, the swine has a coronary artery distribution similar to that of the normal human (Lumb and Hardy, 1963), a limited coronary collateral circulation (Blumgart et al., 1950), and a similar ratio of heart weight to body weight to that of man (Schaper, 1971). Therefore it is an appropriate model for comparison to man (Blumgart et al., 1950; Lumb andSingletary, 1961;1962;Lumb and Hardy, 1963;Kong et al., 1969). However quantitative ultrastructural studies of the normal and hypertrophic heart have been conducted mainly in smaller species, e.g., rats (Page et al., 1971;Anversa et al., 1976Anversa et al., , 1978Legato, 1976; Wendt-Gallitelli and Jacob, 1977;McCallister et al., 1979;Tomanek et al., 1979), rabbits (Anversa et al., 1971;Goldstein et al., 1974), hamsters (Colgan et al., 1978), cats (Sheridan et al., 1977), and ferrets (Breisch et aL, 1980). Although ultrastructural studies have been conducted on the hearts of dogs (Vitali-Mazza and Anversa, 1972;Winkler et al., 1977;McCallister et al., 1978;Legato, 1979), it was not known whether the percentages of cell volume of ultrastructural ...

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Morphometric characterization of left ventricular myocardial cells of male rats during postnatal development

Cited by 24 publications

References 10 publications

Are Age-Related Differences in Response to Myocardial Ischemia and Cardioplegia pH Dependent?

Are Age-Related Differences in Response to Myocardial Ischemia and Cardioplegia pH Dependent?

Redox proteomic identification of HNE-bound mitochondrial proteins in cardiac tissues reveals a systemic effect on energy metabolism after doxorubicin treatment

Myocardial morphometric characteristics in swine.

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