We present a detailed description of the radiological anatomy of the sphenoid sinus and neurovascular structures that surround it from an endoscopic endonasal skull base surgical perspective and in an exclusively Mexican patient population. We performed a cross-sectional study of 500 patients form the Neurosurgery Patient Database at the Specialties Hospital of the 21st Century National Medical Center. Inclusion criteria were minimum age of 18 years old, availability of fine cut CT with skull base reconstruction, absence of skull base pathology, trauma, and radiological artifacts or distortion at the area of study.