Morphometric and ISSR based variability analysis to elucidate population genetic structure in Senecio glaucus L. (Asteraceae: Senecioneae)

Eftekharian, Rosa; Sheidai, Masoud; Attar, Farideh; Noormohammadi, Zahra; Ebadi, Mostafa

doi:10.1007/s13237-016-0182-3

Cited by 2 publications

(3 citation statements)

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“…In our recent study, the genetic structure of S. glaucus showed that population fragmentation, restricted gene flow, genetic drift, and local adaptation have played a role in the genetic divergence of S. glaucus populations in Iran (Eftekharian et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Among molecular markers, inter simple sequence repeat (ISSR) deserves special attention as a tool for analyzing diversity. ISSR is easy to use, quick, simple and highly reproducible (Azizi et al 2014, Eftekharian et al 2016. ISSR markers usually show high polymorphism and have the very important advantage that no prior information about the genomic sequence is required (Kojima et al 1998, Bornet andBranchard 2001).…”

Section: Multivariate Analysismentioning

confidence: 99%

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Morphological and genetic diversity of Senecio vulgaris L. (Asteraceae) in Iran

Ebadi

Eftekharian

2021

Acta bot. Croat. (Online)

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Senecio vulgaris L., an annual herb belonging to the Asteraceae, is widely distributed in different regions of the world. There is no information on the intraspecific variations of the morphological and molecular features of this species. In the present investigation, we studied the morphological and genetic diversity of 81 accessions of S. vulgaris collected from 10 geographical populations. Eleven inter simple sequence repeat (ISSR) primers were used for the examination of genetic variations among the populations. Analysis of molecular variance (AMOVA) and GST analyses revealed significant differences among the investigated populations. A significant correlation between genetic distance and geographical distance was revealed by the Mantel test. However, reticulation analysis indicated the occurrence of gene flow among most of the populations studied. Principal component analysis (PCA) plot showed that the number of capitula, length of the cauline leaf and plant height were the most variable morphological characters. Principal coordinates analysis (PCoA) plot revealed two groups of populations, according to molecular and morphological data. The results suggested the existence of possible intraspecific taxonomic ranks within this species.

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Section: Discussionmentioning

confidence: 99%

Section: Multivariate Analysismentioning

confidence: 99%

Morphological and genetic diversity of Senecio vulgaris L. (Asteraceae) in Iran

Ebadi

Eftekharian

2021

Acta bot. Croat. (Online)

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“…ISSR use microsatellite sequences as primers to amplify genomic regions flanked by microsatellite repeats (Roy et al, 2006). ISSR markers are simple and cost effective to use with high degree of reproducibility as compared with RAPD markers (Eftekharian et al, 2016). The present study was conducted to assess genetic diversity among accessions of Jute mallow germplasm maintained at the World Vegetable Center using ISSR markers.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of jute mallow (corchorus spp.): a traditional African vegetable

Ngomuo¹

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Jute mallow (Corchorus spp.) is a very nutritious traditional leafy vegetable. Its leaves contain an average of 15% dry matter,4.8 g of protein, 259 mg of calcium, 4.5 mg of iron, 4.7 mg of vitamin A, 92 μg of folate, 1.5 mg of nicotinamide and 105 mg of ascorbic acid per 100g of leaves. In spite of its nutritional importance, cultivation of Jute mallow is limited due to lack of scientific information with regard to available cultivars and their adaptation to specific environments. The aim of this study was to analyze the genetic diversity and evaluate the accessions which are well adapted to specific environment for leaf and seeds production. Ninety accessions were evaluated in the field trial experiments for two growing seasons. ISSR and SSR markers were employed for molecular characterization as well as leaf and seed yield evaluation. The results showed significant (p<0.01) variability in accessions in all morphological traits. Traits such as plant height (r=0.448), petiole length (r=0.237), primary branches (r=0.319) and number of leaves per plant (r=0.333) were significantly (p<0.01) correlated with biomass yield. First five Principal components (PCs) with Eigen values ≥1 explained 72.9% of the total variability in the accessions. Cluster analysis grouped the accessions into five major clusters mainly based on their origin. Number of alleles per locus ranged from 2-4 with an average of 2.63 alleles per locus. Polymorphic information content (PIC) ranged from 0.278 in primer SSR17 to 0.78 in primer SSR24. Average Nei‘s gene diversity (h) and Shannon‘s information index (I) were 0.348 and 0.557, respectively. In ISSR markers a total of 85 bands were amplified and average polymorphic bands per primer was 2.75. PIC values ranged from 0.39 to 0.76 with average of 0.53. The highest Nei‘s pair wise genetic distance (0.431) was observed in East African accessions. UPGMA cluster analysis grouped the accessions into five main clusters at genetic similarity coefficient of 0.53. In terms of leaf and seed yield, significant (p<0.01) differences among the accessions in all traits was observed. Leaf fresh weight ranged from 18.3g/plant to 121.3g/plant in accessions TOT 6747 and TOT 8532, respectively. Seed yield ranged from 1.0g/plant to 35.5g/plant in accessions TOT 7980 including eight others and TOT 7866, respectively. Evaluation of genetic diversity and the agronomic traits for leaf and seed yield in this germplasm has revealed useful information for breeders in their efforts to improve the yield as well as selection of accessions with good agronomic traits. It is recommended that detailed study to document ethnomenclacture, ethnobotanical uses, progress towards domestication and challenges faced by farmers be conducted.

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Morphometric and ISSR based variability analysis to elucidate population genetic structure in Senecio glaucus L. (Asteraceae: Senecioneae)

Cited by 2 publications

References 30 publications

Morphological and genetic diversity of Senecio vulgaris L. (Asteraceae) in Iran

Morphological and genetic diversity of Senecio vulgaris L. (Asteraceae) in Iran

Genetic characterization of jute mallow (corchorus spp.): a traditional African vegetable

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