Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process

Stojanović, Vesna; Jovanović, Ivan; Ugrenović, Sladjana; Vasović, Ljiljana; Živković, Vladimir; Jocić, Miodrag; Kundalić, Braca; Pavlović, Miljana

doi:10.1100/2012/845046

Cited by 12 publications

(15 citation statements)

References 15 publications

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“…Glomerular diameter was determined using Feret’s diameter, in concordance with previously published data 62 .…”

Section: Methodsmentioning

confidence: 90%

“…Right: These organoid-derived glomeruli (OrgGloms) when examined in detail (scale bar 100 µm) show similarity to adult human glomeruli (Bottom left, scale bar 100 µm). b OrgGloms are formed in the appropriate size range, comparable to both human glomeruli at 32 weeks gestation 70 , and adult human glomeruli 62 . One-way ANOVA p < 0.0001; error bars = SEM; significant difference assessed by Tukey’s multiple comparisons test; F -value = 176.9; DF = 2; biological replicates n = 30 adult glomeruli, n = 30 neonatal glomeruli, n = 155 OrgGlom.…”

Section: Resultsmentioning

confidence: 99%

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3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening

et al. 2018

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The podocytes within the glomeruli of the kidney maintain the filtration barrier by forming interdigitating foot processes with intervening slit diaphragms, disruption in which results in proteinuria. Studies into human podocytopathies to date have employed primary or immortalised podocyte cell lines cultured in 2D. Here we compare 3D human glomeruli sieved from induced pluripotent stem cell-derived kidney organoids with conditionally immortalised human podocyte cell lines, revealing improved podocyte-specific gene expression, maintenance in vitro of polarised protein localisation and an improved glomerular basement membrane matrisome compared to 2D cultures. Organoid-derived glomeruli retain marker expression in culture for 96 h, proving amenable to toxicity screening. In addition, 3D organoid glomeruli from a congenital nephrotic syndrome patient with compound heterozygous NPHS1 mutations reveal reduced protein levels of both NEPHRIN and PODOCIN. Hence, human iPSC-derived organoid glomeruli represent an accessible approach to the in vitro modelling of human podocytopathies and screening for podocyte toxicity.

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“…Glomerular diameter was determined using Feret’s diameter, in concordance with previously published data 62 .…”

Section: Methodsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening

et al. 2018

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“…In the present study, we conducted a detailed comparative However, some authors hypothesise that the functional renal capacity can't be evaluated by the changes in glomerular size and number, because these morphometric parameters depend on the body size and some sclerotic glomeruli can disappear as age advances [9]. Stojanovi´c et al suggest that glomerular hypertrophy among nonsclerosed glomeruli may be estimated by their connective tissue content [9]. Our study aimed at tracing the changes in two morphometric parameters -area of the renal corpuscles and glomerular area of the superfi cial and juxtamedullary nephrons.…”

Section: Discussionmentioning

confidence: 99%

A comparative morphometric study of the superfi cial and juxtamedullary nephrons during the postnatal development in spontaneously hypertensive rats

Stanchev¹,

Iliev²,

Kotov³

et al. 2018

Arch Anat Physiol

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001Citation: Stanchev S, Iliev A, Kotov G, Malinova L, Landzhov B (2018) A comparative morphometric study of the superficial and juxtamedullary nephrons during the postnatal development in spontaneously hypertensive rats. Arch Anat Physiol 3(1): 001-004. AbstractBackground: Hypertensive nephrosclerosis is associated with progressive loss of functional nephrons leading to chronic renal failure. In our study, we analysed the changes in the areas of the renal corpuscles and glomerular capillary tufts of superfi cial and juxtamedullary nephrons in 9 male spontaneously hypertensive rats, distributed in the following age groups: 4, 6 and 12-month-old. Results:The initial increase in the areas of the renal corpuscles and glomeruli representing the compensatory glomerular hypertrophy was followed by decrease in the late stages of glomerulosclerosis. Our results show that juxtamedullary nephrons were more affected than superfi cial nephrons. Conclusion:Hypertensive nephrosclerosis is associated with both morphological and morphometric changes in nephrons, which lead to declining renal function. Citation:Stanchev S, Iliev A, Kotov G, Malinova L, Landzhov B (2018) A comparative morphometric study of the superficial and juxtamedullary nephrons during the postnatal development in spontaneously hypertensive rats. Arch Anat Physiol 3(1): 001-004. DOI: http://dx.doi.org/10.17352/aap.000010Health (NIH publication No. 86-23, revised 1996). The rats were anaesthetised intraperitoneally with Thiopental 40 mg/ kg b.w. The chest cavity was opened and transcardial perfusion was made with 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.2. Kidneys were quickly removed and fi xed in 10% Publications Pvt. Ltd. Citation: Stanchev S, Iliev A, Kotov G, Malinova L, Landzhov B (2018) A comparative morphometric study of the superficial and juxtamedullary nephrons during the postnatal development in spontaneously hypertensive rats. Arch Anat Physiol 3(1): 001-004.

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“…Images of the histological slices of Sirius red stain were analyzed and captured under 100× magnification. Further Area of their fibrosis was morphometrically and statistically analyzed with Image J and SPSS software [19] .…”

Section: Methodsmentioning

confidence: 99%

Metformin Prevents and Reverses Inflammation in a Non-Diabetic Mouse Model of Nonalcoholic Steatohepatitis

et al. 2012

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BackgroundOptimal treatment for nonalcoholic steatohepatitis (NASH) has not yet been established, particularly for individuals without diabetes. We examined the effects of metformin, commonly used to treat patients with type 2 diabetes, on liver pathology in a non-diabetic NASH mouse model.Methodology/Principal FindingsEight-week-old C57BL/6 mice were fed a methionine- and choline-deficient plus high fat (MCD+HF) diet with or without 0.1% metformin for 8 weeks. Co-administration of metformin significantly decreased fasting plasma glucose levels, but did not affect glucose tolerance or peripheral insulin sensitivity. Metformin ameliorated MCD+HF diet-induced hepatic steatosis, inflammation, and fibrosis. Furthermore, metformin significantly reversed hepatic steatosis and inflammation when administered after the development of experimental NASH.Conclusions/SignificanceThese histological changes were accompanied by reduced hepatic triglyceride content, suppressed hepatic stellate cell activation, and the downregulation of genes involved in fatty acid metabolism, inflammation, and fibrogenesis. Metformin prevented and reversed steatosis and inflammation of NASH in an experimental non-diabetic model without affecting peripheral insulin resistance.

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Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process

Cited by 12 publications

References 15 publications

3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening

3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening

A comparative morphometric study of the superfi cial and juxtamedullary nephrons during the postnatal development in spontaneously hypertensive rats

Metformin Prevents and Reverses Inflammation in a Non-Diabetic Mouse Model of Nonalcoholic Steatohepatitis

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