Morphometric analysis of fetal development of Cavia porcellus (Linnaeus, 1758) by ultrasonography—Pilot study

Santos, Juliana Passos Alves dos; Fonseca, Érika Toledo da; Melis, Juliano van; Miglino, María Angélica

doi:10.1016/j.theriogenology.2014.01.004

Cited by 3 publications

(5 citation statements)

References 17 publications

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“…The transversal view allowed us to capture a longitudinal and transverse axis, where we visualized the decidua and the exchange placental region. This observation and histological studies of placentas at G35 and G60 are consistent with Santos et al [ 17 ], likely reflecting changes in lobule formation and vascular organization of the guinea pig placenta toward term. Moreover, histological inspection of the placenta confirmed the involution of the subplacenta across pregnancy as it was no longer apparent at G60 [ 41 ].…”

Section: Discussionsupporting

confidence: 91%

“…Previously, Turner and Trudinger (2000) [ 35 ], and Santos et al (2014) [ 17 ] described the normal growth of BPD in guinea pigs from G22 until the end of pregnancy. Turner and Trudinger presented these data as a mean of 3–5 day intervals, whereas Santos et al showed a mean for every 5 days of gestation.…”

Section: Discussionmentioning

confidence: 99%

“…Few studies have used ultrasound to describe biparietal diameter (BPD) and the resistance index of the umbilical artery during guinea pig pregnancy (G22 to G69) [ 16 ]. Further studies have analyzed other biometric parameters, such as abdominal and placental diameter with ultrasound imaging in guinea pigs [ 17 , 18 ]. Lastly, studies using sonography have reported that UA and UtA PI are altered in guinea pig pregnancies with placental insufficiency [ 15 ], with changes similar to what is seen in humans.…”

Section: Introductionmentioning

confidence: 99%

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Developmental Ultrasound Characteristics in Guinea Pigs: Similarities with Human Pregnancy

Candia

Jiménez

Navarrete

et al. 2023

Veterinary Sciences

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Background: Biometrical and blood flow examinations are fundamental for assessing fetoplacental development during pregnancy. Guinea pigs have been proposed as a good model to study fetal development and related gestational complications; however, longitudinal growth and blood flow changes in utero have not been properly described. This study aimed to describe fetal and placental growth and blood flow of the main intrauterine vascular beds across normal guinea pig pregnancy and to discuss the relevance of this data for human pregnancy. Methods: Pregnant guinea pigs were studied from day 25 of pregnancy until term (day ~70) by ultrasound and Doppler assessment. The results were compared to human data from the literature. Results: Measurements of biparietal diameter (BPD), cranial circumference (CC), abdominal circumference, and placental biometry, as well as pulsatility index determination of umbilical artery, middle cerebral artery (MCA), and cerebroplacental ratio (CPR), were feasible to determine across pregnancy, and they could be adjusted to linear or nonlinear functions. In addition, several of these parameters showed a high correlation coefficient and could be used to assess gestational age in guinea pigs. We further compared these data to ultrasound variables from human pregnancy with high similarities. Conclusions: BPD and CC are the most reliable measurements to assess fetal growth in guinea pigs. Furthermore, this is the first report in which the MCA pulsatility index and CPR are described across guinea pig gestation. The guinea pig is a valuable model to assess fetal growth and blood flow distribution, variables that are comparable with human pregnancy.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Developmental Ultrasound Characteristics in Guinea Pigs: Similarities with Human Pregnancy

Candia

Jiménez

Navarrete

et al. 2023

Veterinary Sciences

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“…Crown–rump length can be assessed up to GD35 but after that time the fetus is too long and flexed for accurate measurement (Santos et al . ; Swanson et al . ).…”

Section: Introductionmentioning

confidence: 99%

Guinea pig models for translation of the developmental origins of health and disease hypothesis into the clinic

Morrison

Botting

Darby

et al. 2018

The Journal of Physiology

117

108

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Over 30 years ago Professor David Barker first proposed the theory that events in early life could explain an individual's risk of non-communicable disease in later life: the developmental origins of health and disease (DOHaD) hypothesis. During the 1990s the validity of the DOHaD hypothesis was extensively tested in a number of human populations and the mechanisms underpinning it characterised in a range of experimental animal models. Over the past decade, researchers have sought to use this mechanistic understanding of DOHaD to develop therapeutic interventions during pregnancy and early life to improve adult health. A variety of animal models have been used to develop and evaluate interventions, each with strengths and limitations. It is becoming apparent that effective translational research requires that the animal paradigm selected mirrors the tempo of human fetal growth and development as closely as possible so that the effect of a perinatal insult and/or therapeutic intervention can be fully assessed. The guinea pig is one such animal model that over the past two decades has demonstrated itself to be a very useful platform for these important reproductive studies. This review highlights similarities in the in utero development between humans and guinea pigs, the strengths and limitations of the guinea pig as an experimental model of DOHaD and the guinea pig's potential to enhance clinical therapeutic innovation to improve human health.

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“…The samples were collected from multiple mothers. The guinea pig pregnancy age estimation was performed based in [ 27 , 28 ]. The animals were submitted to vaginal cytology to detect the onset of pregnancy, and ultrasound examination to monitor gestational development.…”

Section: Methodsmentioning

confidence: 99%

The comparative aspects of hystricomorph subplacenta: potential endocrine organ

Miglino¹,

Matias²,

Rigoglio³

et al. 2021

BMC Zool

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Background The placenta of hystricomorph rodents, lagomorphs and some primates includes an unusual structure, termed a subplacenta, which essentially consists of trophoblastic cells located deep to the central implantation site within the area of decidualization. It has been suggested that the subplacenta is functionally important, although considerable controversy remains on the issue. In this context, our objective was to compare the architecture and structure of the subplacentas of different hystricomorph species, to investigate the possibility that it is active in hormone synthesis. Methods In total, the placentas of 3 capybaras (Hydrochaeris hydrochaeris), 2 pacas (Agouti paca), 5 agoutis (Dasyprocta leporina), 5 rock cavies (Kerodon rupestris) and 3 guinea pigs (Cavia porcellus) at different stages of pregnancy (early, middle and near term) were used for gross and microscopic examination. This included the preparation of latex injection casts, immunohistochemistry for steroidogenic enzymes, scanning and transmission electron microscopy. Tissue steroid concentrations were also determined. Results The gross morphology and microvascular arrangement of the subplacentas were similar among the hystricomorphs studied including ultra-structural verification of cytotrophoblast and syncytiotrophoblast in all species. In guinea pigs, trophoblast cells exhibited characteristics consistent with intense metabolic and secretory activity in general. However, immuno-histochemical evidence also indicated that subplacental trophoblast expressed key steroidogenic enzymes, mainly in the chorionic villus region, consistent with tissue steroid concentrations. Conclusions The subplacentas within placentas of hystricomorph rodent species are structurally similar and, in guinea pigs, have potential for steroid hormone secretion from, at least the early stages of pregnancy.

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Morphometric analysis of fetal development of Cavia porcellus (Linnaeus, 1758) by ultrasonography—Pilot study

Cited by 3 publications

References 17 publications

Developmental Ultrasound Characteristics in Guinea Pigs: Similarities with Human Pregnancy

Developmental Ultrasound Characteristics in Guinea Pigs: Similarities with Human Pregnancy

Guinea pig models for translation of the developmental origins of health and disease hypothesis into the clinic

The comparative aspects of hystricomorph subplacenta: potential endocrine organ

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