Morphometric analysis of basal laminae in rats with long-term streptozotocin diabetes L. II. Retinal capillaries

“…EM studies on the STZ-injected rat have revealed thicker BM after 12 months. 12–13 The response to STZ-induced hyperglycemia seems more variable in the mouse depending on the strain. While diabetic BALB/c mice had thicker BM after 20 months, STZ injected C57Bl/6J mice did not have any change in the BM, even after 18 months.…”

Section: Discussionmentioning

confidence: 99%

“…19 Ashton also emphasized that the basal lamina of the pericyte does not usually share in the thickening of the whole basal lamina in DR. 2 The basal lamina thickening in retinal capillaries has been attributed to glial cells, implying a disturbance of Muller cell metabolism in diabetes. 2, 13, 20–21 In this study, we measured only the outer basal lamina which exists between the glial cells and pericyte or endothelium, excluding the inner basal lamina, as the outer basal lamina is a more reliable indicator of BM thickness in DR and shows more dramatic change associated with the metabolic activity of Muller cells.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Features of Retinal Capillary Basement Membrane Thickening in Diabetic Swine

Lee

Rattigan

et al. 2010

Ultrastructural Pathology

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Purpose To evaluate the ultrastructural features of retinal capillary basement membranes (BMs) in a swine model of type 1 diabetes. Materials and Methods Yorkshire pigs were rendered diabetic with streptozotocin and dyslipidemic with a high fat and cholesterol diet. At 18, 26 and 32 weeks of diabetes, the retina sections within 3 disc diameters from the optic disc were examined under transmission electron microscope to evaluate the ultrastructural features of retinal capillary BM. For measurement of the thickness of these capillary BMs, digital morphometric analysis was adapted. Results Diabetic swine had significantly thicker retinal capillary BMs compared to controls. Pigs that sustained diabetes for longer periods or experienced severe diabetes tended to have thicker BMs. Those pigs which did not maintain glucose levels above 200 mg/dL did not demonstrate thicker retinal capillary BMs. Characteristic ultrastructural features of diabetic vasculopathy were observed including rarefaction as an early stage of Swiss cheese cavitation, lamellation with multiplication of electron dense layers, and fibrillar materials within capillary BM. Conclusions Diabetic Yorkshire pigs develop characteristic features of an early retinal microangiopathy fairly rapidly and may serve as a higher order animal model for basement membrane studies of type 1 diabetes.

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“…Where the radial lines intersected the BL, BL thickness was measured. Both "inner" and "outer" BLs were defined according to Fischer and Gärtner (1983). The "inner" BM is positioned in between endothelial cell and pericyte as joint BL (jBL).…”

Section: Determination Of Bl Thicknessmentioning

confidence: 99%

“…Capillaries were excluded from the study when <12 measurements could be made. In addition, a second approach was followed to blindly measure the BL thickness in the same 10 randomly selected images of capillaries as described above according to Fischer and Gärtner (1983). In this case, the BL of each capillary was divided into three regions: BL of the endothelial cell (eBL), BL of the pericyte (pBL), and joint BL of both endothelial cell and pericyte (jBL) (Figure 1).…”

Section: Determination Of Bl Thicknessmentioning

confidence: 99%

Connective Tissue Growth Factor Is Necessary for Retinal Capillary Basal Lamina Thickening in Diabetic Mice

Kuiper

Zijderveld

Roestenberg

et al. 2008

J Histochem Cytochem.

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S U M M A R Y Experimental prevention of basal lamina (BL) thickening of retinal capillaries ameliorates early vascular changes caused by diabetes. Connective tissue growth factor (CTGF) is upregulated early in diabetes in the human retina and is a potent inducer of expression of BL components. We hypothesize that CTGF is causally involved in diabetesinduced BL thickening of retinal capillaries. To test this hypothesis, we compared the effects of streptozotocin (STZ)-induced diabetes on retinal capillary BL thickness between wild-type mice (CTGF 1/1 ) and mice lacking one functional CTGF allele (CTGF 1/2 ). Differences in BL thickness were calculated by quantitative analysis of electron microscopic images of transversally sectioned capillaries in and around the inner nuclear layer of the retina. We show that BL thickening was significant in diabetic CTGF

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Morphometric analysis of basal laminae in rats with long-term streptozotocin diabetes L. II. Retinal capillaries

Cited by 23 publications

References 19 publications

The Role of CTGF in Diabetic Retinopathy

The Role of CTGF in Diabetic Retinopathy

Ultrastructural Features of Retinal Capillary Basement Membrane Thickening in Diabetic Swine

Connective Tissue Growth Factor Is Necessary for Retinal Capillary Basal Lamina Thickening in Diabetic Mice

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