Morphology transition of raft-model membrane induced by osmotic pressure: Formation of double-layered vesicle similar to an endo- and/or exocytosis

Onai, Teruaki; Hirai, Mitsuhiro

doi:10.1088/1742-6596/247/1/012018

Cited by 9 publications

(17 citation statements)

References 33 publications

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“…As shown previously (Onai & Hirai, 2010), the scattering curve of an empty-LUV can be characterized by the shoulder at $ 0.01 Å À1 and the broad rounded peak at $ 0.04-0.2 Å À1 . The former and the latter correspond to the spherical shape of LUVs with a size distribution (polydispersity) and to an internal structure of the bilayer, respectively.…”

Section: Shell-modeling Analysis and Simulation Of Luvssupporting

confidence: 65%

“…Owing to the simple structural symmetry of vesicles, the shell-model scattering function given by equations (2) and (5) is applicable for describing experimental scattering curves in many cases (Hirai et al, 2003(Hirai et al, , 2013Hirai, 2007;Onai & Hirai, 2010). Fig.…”

Section: Shell-modeling Analysis and Simulation Of Luvsmentioning

confidence: 99%

“…6 shows the contrast profile of the intra-bilayer structure of the empty-LUV obtained from the modeling analysis. The low positive-contrast region at the outer-leaflet of the bilayer corresponds to the protruded portion of the oligosaccharide chains from the membrane surface, which is the common feature for LUVs containing ganglioside molecules (Hirai et al, 2003(Hirai et al, , 2006(Hirai et al, , 2013Hirai, 2007;Onai & Hirai, 2010). Such an asymmetry of the bilayer structure results from the difference in the geometrical packing parameters of lipid molecules.…”

Section: Shell-modeling Analysis and Simulation Of Luvsmentioning

confidence: 99%

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Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling

et al. 2013

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Lipid liposomes are promising drug delivery systems because they have superior curative effects owing to their high adaptability to a living body. Lipid liposomes encapsulating proteins were constructed and the structures examined using synchrotron radiation small-and wide-angle X-ray scattering (SR-SWAXS). The liposomes were prepared by a sequential combination of natural swelling, ultrasonic dispersion, freeze-throw, extrusion and spin-filtration. The liposomes were composed of acidic glycosphingolipid (ganglioside), cholesterol and phospholipids. By using shell-modeling methods, the asymmetric bilayer structure of the liposome and the encapsulation efficiency of proteins were determined. As well as other analytical techniques, SR-SWAXS and shellmodeling methods are shown to be a powerful tool for characterizing in situ structures of lipid liposomes as an important candidate of drug delivery systems.

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Section: Shell-modeling Analysis and Simulation Of Luvssupporting

confidence: 65%

Section: Shell-modeling Analysis and Simulation Of Luvsmentioning

confidence: 99%

Section: Shell-modeling Analysis and Simulation Of Luvsmentioning

confidence: 99%

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Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling

et al. 2013

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“…24,25,27,34 The sharp peak at ~1.491 Å -1 indicates that the alkyl-chains of the lipid bilayer is in gel phase. 35, 36…”

Section: Hierarchical Structure Of Liposome Analyzed By Shell-modelinmentioning

confidence: 99%

Effect of Protein-Encapsulation on Thermal Structural Stability of Liposome Composed of Glycosphingolipid/Cholesterol/Phospholipid

et al. 2015

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We have studied the thermal structural stability of liposomes encapsulating proteins by using synchrotron radiation small- and wide-angle X-ray scattering (SR-SWAXS). Liposomes are known to be effective drug-delivery systems (DDSs) because they can reduce drug toxicity due to biodegradability and biocompatibility and can offer promising carriers of various types of drugs. However, in spite of numerous studies of liposomes, physicochemical characteristics of liposomes entrapping proteins are rarely known. The liposome studied is characterized by the lipid composition (mixture of acidic glycosphingolipid (ganglioside)/cholesterol/phospholipid). Gangliosides are one of the major constituents of so-called lipid rafts playing the role of a platform of cell-signaling. We have found that the encapsulation of proteins elevates the thermal transition temperature of the liposome membrane and suppresses the deformation of its shape. The present results suggest that not only membrane proteins, but also water-soluble proteins affect liposome stability through the revalence between osmotic pressure and membrane elasticity. In addition, we have found the presence of the size-effect depending on the molar content of gangliosides in the liposome, indicating the ability of ganglioside molecule controlling both the size and effective surface charge of the liposome. The present results would have significance from two different points of view. One is the confinement effect of proteins within a limited space like cell, and the other is a stability of a new type of DDS using gangliosides. Due to the intrinsic properties, gangliosides are expected to be promising agents for targeting and long-circulation properties of liposomal DDSs.

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“…A common feature of lipid rafts is their peculiar lipid composition, being rich in glycosphingolipids (GSLs), sphingomyelin and cholesterol. Functions of lipid rafts are assumed to relate closely to the peculiar features of GSL molecules both in ceramide and oligosaccharide portions that can form complex hydrogen bonding networks (hydrogen bond donor and acceptor) [ 7 ]. GM1 refers to the GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, but occurring in highest concentrations in the central nervous system.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes

et al. 2013

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It is important to understand the physicochemical mechanisms that are responsible for the morphological changes in the cell membrane in the presence of various stimuli such as osmotic pressure. Lipid rafts are believed to play a crucial role in various cellular processes. It is well established that Ctb (Cholera toxin B subunit) recognizes and binds to GM1 (monosialotetrahexosylganglioside) on the cell surface with high specificity and affinity. Taking advantage of Ctb-GM1 interaction, we examined how Ctb and GM1 molecules affect the dynamic movement of liposomes. GM1 a natural ligand for cholera toxin, was incorporated into liposome and the interaction between fluorescent Ctb and the liposome was analyzed. The interaction plays an important role in determining the various surface interaction phenomena. Incorporation of GM1 into membrane leads to an increase of the line tension leading to either rupture of liposome membrane or change in the morphology of the membrane. This change in morphology was found to be GM1 concentration specific. The interaction between Ctb-GM1 leads to fast and easy rupture or to morphological changes of the liposome. The interactions of Ctb and the glycosyl chain are believed to affect the surface and the curvature of the membrane. Thus, the results are highly beneficial in the study of signal transduction processes.

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Morphology transition of raft-model membrane induced by osmotic pressure: Formation of double-layered vesicle similar to an endo- and/or exocytosis

Cited by 9 publications

References 33 publications

Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling

Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling

Effect of Protein-Encapsulation on Thermal Structural Stability of Liposome Composed of Glycosphingolipid/Cholesterol/Phospholipid

Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes

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