Morphology study of DMPC/DHPC mixtures by solution-state 1H, 31P NMR, and NOE measurements

Shintani, Megumi; Matubayasi, Nobuyuki

doi:10.1016/j.molliq.2015.10.003

Cited by 6 publications

(6 citation statements)

References 36 publications

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“…Overall, the QCM-D shifts indicated the SLB formation, with final Δf and ΔD values around − 25 to − 28 Hz and 0.4 to 1.5 × 10 -6 , respectively. At this q-ratio, SLB formation likely involved the adsorption and spontaneous rupture of spherical bicelles rather than discoidal bicelles as at q = 0.05 and 0.25, since DOPC/DHPC bicelles 49 and related ones 50 have been shown to possess a spherical shape at similar q-ratios. Thus, the QCM-D data support that DOPC/CA bicelles at q = 2.5 can form SLBs at all tested concentrations while optimal conditions corresponded to 0.13-0.016 mM DOPC on account of low ΔD values that are acceptable for good-quality SLBs based on generally practiced standards used in the field, i.e., around or less than 1 × 10 -6 ( Fig.…”

Section: Resultsmentioning

confidence: 94%

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Sut

Yoon

Park

et al. 2020

Sci Rep

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Originally developed for the structural biology field, lipid bicelle nanostructures composed of longand short-chain phospholipid molecules have emerged as a useful interfacial science tool to fabricate two-dimensional supported lipid bilayers (SLBs) on hydrophilic surfaces due to ease of sample preparation, scalability, and versatility. To improve SLB fabrication prospects, there has been recent interest in replacing the synthetic, short-chain phospholipid component of bicellar mixtures with naturally abundant fatty acids and monoglycerides, i.e., lauric acid and monocaprin. Such options have proven successful under specific conditions, however, there is room for devising more versatile fabrication options, especially in terms of overcoming lipid concentration-dependent SLB formation limitations. Herein, we investigated SLB fabrication by using bicellar mixtures consisting of long-chain phospholipid and capric acid, the latter of which has similar headgroup and chain length properties to lauric acid and monocaprin, respectively. Quartz crystal microbalance-dissipation, epifluorescence microscopy, and fluorescence recovery after photobleaching experiments were conducted to characterize lipid concentration-dependent bicelle adsorption onto silicon dioxide surfaces. We identified that uniform-phase SLB formation occurred independently of total lipid concentration when the ratio of long-chain phospholipid to capric acid molecules ("q-ratio") was 0.25 or 2.5, which is superior to past results with lauric acid-and monocaprin-containing bicelles in which cases lipid concentration-dependent behavior was observed. Together, these findings demonstrate that capric acid-containing bicelles are versatile tools for SLB fabrication and highlight how the molecular structure of bicelle components can be rationally finetuned to modulate self-assembly processes at solid-liquid interfaces. Bicelles are an important class of membrane-mimicking lipid nanostructures that self-assemble from mixtures of long-and short-chain phospholipids under appropriate processing conditions 1,2. Also known as lipid nanodisks 3 or bilayered mixed micelles 4,5 , bicelles can exist in a wide range of morphologies (e.g., perforated sheets and wormlike micelles) depending on parameters such as temperature, q-ratio (long-to short-chain phospholipid molar ratio), total lipid concentration, and lipid composition, and are widely conceptualized as two-dimensional disks whereby long-chain phospholipids constitute a planar lipid bilayer surface and the short-chain phospholipids form a rimmed edge around the bilayer 6-12. Since they can exhibit magnetic alignment in some cases, bicellar disks have long been used in the nuclear magnetic resonance spectroscopy field as membrane protein hosts 4,13-16. In the interfacial science field, bicelles have also proven to be useful as a lipid nanostructure to fabricate supported lipid bilayers (SLBs), which are extensively used in applications such as biosensors and micropatterned arrays 17-25. Indeed, bicelle adsorption onto...

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Section: Resultsmentioning

confidence: 94%

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Sut

Yoon

Park

et al. 2020

Sci Rep

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“…Overall, the results demonstrate that SLB formation is favorable at q = 2.5 with all concentrations (0.5 mM DOPC and below). As with DOPC/DHPC bicelles and DMPC/DHPC bicelles at similar q -ratios, the DOPC/LA bicelles likely exist in a spherical form at q = 2.5, which is conducive to the SLB formation process akin to vesicle fusion involving spherical vesicles while the presence of short-chain lipids has also been noted to induce detergent-mediated membrane softening …”

Section: Resultsmentioning

confidence: 96%

Supported Lipid Bilayer Formation from Phospholipid-Fatty Acid Bicellar Mixtures

et al. 2020

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“…Bicelles vary in size and shape depending on the ratio of lipid to detergent (known as the q value), 34 the structure of the lipid and detergent monomers, 25 total concentration of amphiphiles, 35,36 and temperature. 29,30 For solution NMR structural studies, bicelles with low q values (<0.7; also known as fast-tumbling “isotropic” bicelles) have demonstrated some utility for polytopic integral membrane proteins. 24,31,37,38 Several of these studies suggest that the stabilization of membrane protein fold is due to the more “bilayer” nature of bicelles compared with micelles.…”

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confidence: 99%

“…Bicelle self-assembly was first determined in 1984, , and since then, bicelles have been characterized using many methods such as small-angle neutron scattering (SANS) , and nuclear magnetic resonance (NMR). − The classically described (“ideal”) bicelle contains a central disk-shaped lipid bilayer encircled by a rim of detergents that screen the hydrophobic lipid tails from water (Figure C). , Thus, in the “ideal” bicelle, the lipid and detergent molecules are segregated spatially. Bicelles vary in size and shape depending on the ratio of lipid to detergent (known as the q value), the structure of the lipid and detergent monomers, total concentration of amphiphiles, , and temperature. , For solution NMR structural studies, bicelles with low q values (<0.7; also known as fast-tumbling “isotropic” bicelles) have demonstrated some utility for polytopic integral membrane proteins. ,,, Several of these studies suggest that the stabilization of membrane protein fold is due to the more “bilayer” nature of bicelles compared with micelles. That is, the segregated lipid core in bicelles is more similar in structure to the native membrane.…”

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confidence: 99%

Low-q Bicelles Are Mixed Micelles

Caldwell

Baoukina

Brock

et al. 2018

J. Phys. Chem. Lett.

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Bicelles are used in many membrane protein studies because they are thought to be more bilayer-like than micelles. We investigated the properties of "isotropic" bicelles by small-angle neutron scattering, small-angle X-ray scattering, fluorescence anisotropy, and molecular dynamics. All data suggest that bicelles with a q value below 1 deviate from the classic bicelle that contains lipids in the core and detergent in the rim. Thus not all isotropic bicelles are bilayer-like.

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Morphology study of DMPC/DHPC mixtures by solution-state 1H, 31P NMR, and NOE measurements

Cited by 6 publications

References 36 publications

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Supported Lipid Bilayer Formation from Phospholipid-Fatty Acid Bicellar Mixtures

Low-q Bicelles Are Mixed Micelles

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