Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan

Wakeman, Kevin C.; Yamaguchi, Aika; Roy, Michael C.; Jenke‐Kodama, Holger

doi:10.1016/j.hal.2015.02.009

Cited by 41 publications

(42 citation statements)

References 39 publications

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“…Based on the number of PKS domains observed per million reads of the transcriptome, surprisingly, the greatest concentration of PKS domains were observed in C. malayensis (Table 1). While C. malayensis is not known to produce PLTX-like compounds, at least five, mostly unnamed polyketide compounds are produced by this species, and the diversity of PKS domains in this species suggests the potential for the production of many more (Wakeman et al, 2015).…”

Section: Discussionmentioning

confidence: 96%

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Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species

Verma

Kohli

Harwood

et al. 2019

Environmental Microbiology

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Summary In marine ecosystems, dinoflagellates can become highly abundant and even dominant at times, despite their comparatively slow growth. Their ecological success may be related to their production of complex toxic polyketide compounds. Ostreopsis species produce potent palytoxin‐like compounds (PLTX), which are associated with human skin and eye irritations, and illnesses through the consumption of contaminated seafood. To investigate the genetic basis of PLTX‐like compounds, we sequenced and annotated transcriptomes from two PLTX‐producing Ostreopsis species; O. cf. ovata, O. cf. siamensis, one non‐PLTX producing species, O. rhodesae and compared them to a close phylogenetic relative and non‐PLTX producer, Coolia malayensis. We found no clear differences in the presence or diversity of ketosynthase and ketoreductase transcripts between PLTX producing and non‐producing Ostreopsis and Coolia species, as both groups contained >90 and > 10 phylogenetically diverse ketosynthase and ketoreductase transcripts, respectively. We report for the first‐time type I single‐, multi‐domain polyketide synthases (PKSs) and hybrid non‐ribosomal peptide synthase/PKS transcripts from all species. The long multi‐modular PKSs were insufficient by themselves to synthesize the large complex polyether backbone of PLTX‐like compounds. This implies that numerous PKS domains, including both single and multi‐, work together on the biosynthesis of PLTX‐like and other related polyketide compounds.

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Section: Discussionmentioning

confidence: 96%

“…While C . malayensis is not known to produce PLTX‐like compounds, at least five, mostly unnamed polyketide compounds are produced by this species, and the diversity of PKS domains in this species suggests the potential for the production of many more (Wakeman et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species

Verma

Kohli

Harwood

et al. 2019

Environmental Microbiology

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“…More recently, Wakeman et al. () characterized five more analogs of yessotoxin, distinct from cooliatoxin, from a strain of C. malayensis (Okinawa, Japan). Despite proving lethal to mice (Carlson et al.…”

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confidence: 99%

First description of the environmental niche of the epibenthic dinoflagellate species Coolia palmyrensis, C. malayensis, and C. tropicalis (Dinophyceae) from Eastern Australia

Larsson

Smith

Doblin

2019

Journal of Phycology

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Environmental variables such as temperature, salinity, and irradiance are significant drivers of microalgal growth and distribution. Therefore, understanding how these variables influence fitness of potentially toxic microalgal species is particularly important. In this study, strains of the potentially harmful epibenthic dinoflagellate species Coolia palmyrensis, C. malayensis, and C. tropicalis were isolated from coastal shallow water habitats on the east coast of Australia and identified using the D1‐D3 region of the large subunit (LSU) ribosomal DNA (rDNA). To determine the environmental niche of each taxon, growth was measured across a gradient of temperature (15–30°C), salinity (20–38), and irradiance (10–200 μmol photons · m−2 · s−1). Specific growth rates of Coolia tropicalis were highest under warm temperatures (27°C), low salinities (ca. 23), and intermediate irradiance levels (150 μmol photons · m−2 · s−1), while C. malayensis showed the highest growth at moderate temperatures (24°C) and irradiance levels (150 μmol photons · m−2 · s−1) and growth rates were consistent across the range of salinity levels tested (20–38). Coolia palmyrensis had the highest growth rate of all species tested and favored moderate temperatures (24°C), oceanic salinity (35), and high irradiance (>200 μmol photons · m−2 · s−1). This is the first study to characterize the environmental niche of species from the benthic harmful algal bloom genus Coolia and provides important information to help define species distributions and inform risk management.

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“…The species of the genus Coolia Meunier are an important component of benthic dinoflagellate assemblages, especially in tropical and subtropical waters (Karafas et al ; Wakeman et al ). The type species, C. monotis Meunier, was described in the temperate waters and other species were described in tropical waters: C. tropicalis M. A. Faust, C. areolata Ten‐Hage, J. Turquet, J. P. Quod & Couté and C. canariensis S. Fraga (Meunier ; Faust ; Ten‐Hage et al ; Fraga et al ).…”

mentioning

confidence: 99%

Circumtropical distribution of the epiphytic dinoflagellate Coolia malayensis (Dinophyceae): Morphology and molecular phylogeny from Puerto Rico and Brazil

Gómez

Qiu

Otero

et al. 2016

Phycological Research

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SUMMARY The dinoflagellate genus Coolia, which contains potentially toxic species, is an important component of epiphytic assemblages in marine ecosystems. The morphology of C. malayensis has been illustrated from strains isolated in Asia and Oceania. In this study, strains of C. malayensis isolated from the Caribbean Sea in Puerto Rico, and for the first time from the South Atlantic Ocean in Brazil, were investigated by light, epifluorescence and scanning electron microscopies. No significant morphological differences between these new strains and other geographically distant strains of C. malayensis were observed. In the LSU rDNA phylogeny, the C. malayensis sequences from Brazil and Puerto Rico branched within the clade of strains from Oceania and Asia. The recently described species C. santacroce branched as a sister group of C. monotis, and C. palmyrensis was basal to the combined group of C. monotis/C. malayensis/C. santacroce. A tentative undescribed species from Florida and New Zealand branched as a sister group of C. malayensis. Our results confirm that C. malayensis showed a cosmopolitan distribution in tropical to subtropical waters, while the type species C. monotis remains endemic for the Mediterranean Sea and the temperate North Atlantic.

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Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan

Cited by 41 publications

References 39 publications

Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species

Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species

First description of the environmental niche of the epibenthic dinoflagellate species Coolia palmyrensis, C. malayensis, and C. tropicalis (Dinophyceae) from Eastern Australia

Circumtropical distribution of the epiphytic dinoflagellate Coolia malayensis (Dinophyceae): Morphology and molecular phylogeny from Puerto Rico and Brazil

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