Morphology of the rat peritoneum after carbon dioxide and helium pneumoperitoneum: a scanning electron microscopic study

Ordemann, J.; Jakob, Jens; Braumann, Chris; Kilian, M.; Bachmann, S.; Jacobi, C. A.

doi:10.1007/s00464-003-9217-3

Cited by 11 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Authors described bulging of mesothelial cells, irregular cell junction’s cell membrane degradation and mesodermal edema [9]. These findings were in accordance with other studies that evaluated the influence of pneumoperitoneum on peritoneal tissue using scanning electron microscopy [10, 11]. However, we were unable to find studies that evaluated the changes in the collagenous structure of basal network.…”

supporting

confidence: 89%

Does thermodynamic stability of peritoneal collagen change during laparoscopic cholecystectomies? A differential scanning calorimetry (DSC) study

et al. 2014

View full text Add to dashboard Cite

BackgroundCarbon dioxide pneumoperitoneum used during laparoscopic surgeries alters the integrity of the peritoneum and results in denudation of the basal lamina that might cause altered immune response, inhibited fibrinolysis, hypoxia, and acidosis. The changes in the structure of pneumoperitoneum were described as bulging of mesothelial cells, irregular cell junction’s cell membrane degradation, and mesodermal edema. As denaturation of peritoneal proteins reflects overall condition of its structure and interactions with the surrounding molecules, the physical status of collagen was assessed on the basis of parameters of thermal denaturation measured by DSC method.MethodsTwenty-four female patients operated on due to cholelithiasis were enrolled in this study. Laparoscopic cholecystectomy was performed using standard four-trocar technique, and standard values of insufflated carbon dioxide pneumoperitoneum were used. After trocar placement, the first collection of peritoneal sample (sample A) was performed. The second peritoneal sample (sample B) was collected after the removal of gall bladder. Differential scanning calorimetry (Q200 calorimeter, TA Instruments) was performed on samples defrosted at room temperature.ResultsIn all samples of peritoneum, a nonreversible endothermal process recognized as denaturation was observed. Sample B obtained at the end of surgery did not differ from sample A obtained at the beginning in terms of all parameters under study. Temperature of denaturation in A and B was correlated only marginally, but enthalpy and specific heat were significantly correlated. The analysis of data from DSC measurements did not reveal differences in physical stability of collagen in peritoneal samples obtained at the beginning and at the end of surgery. Significant negative correlations between duration of CO2 pneumoperitoneum and enthalpy of denaturation in sample B were found.ConclusionsDifferences in enthalpy of denaturation may reflect a quantitative relation between amount of native collagen molecules in the sample and other, non-collagenous components or impaired collagen.

show abstract

supporting

confidence: 89%

Does thermodynamic stability of peritoneal collagen change during laparoscopic cholecystectomies? A differential scanning calorimetry (DSC) study

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Within hours transforming growth factor b increases and tissue plasminogen activator decreases. By retraction, the contiguous mesothelial cells are transformed into individual cells which causes passive diffusion through the exposed basal membrane (31,(39)(40)(41)(42) and initiates acute inflammation (43). The peritoneal cavity thus becomes part of the body, which is an efficient defense mechanism against infection via recruiting immunoglobulins and macrophages.…”

Section: The Peritoneal Cavity Is An Organmentioning

confidence: 99%

Role of the peritoneal cavity in the prevention of postoperative adhesions, pain, and fatigue

Koninckx¹,

Gomel

Ussia³

et al. 2016

Fertility and Sterility

109

View full text Add to dashboard Cite

A surgical trauma results within minutes in exudation, platelets, and fibrin deposition. Within hours, the denuded area is covered by tissue repair cells/macrophages, starting a cascade of events. Epithelial repair starts on day 1 and is terminated by day 3. If repair is delayed by decreased fibrinolysis, local inflammation, or factors in peritoneal fluid, fibroblast growth starting on day 3 and angiogenesis starting on day 5 results in adhesion formation. For adhesion formation, quantitatively more important are factors released into the peritoneal fluid after retraction of the fragile mesothelial cells and acute inflammation of the entire peritoneal cavity. This is caused by mechanical trauma, hypoxia (e.g., CO pneumoperitoneum), reactive oxygen species (ROS; e.g., open surgery), desiccation, or presence of blood, and this is more severe at higher temperatures. The inflammation at trauma sites is delayed by necrotic tissue, resorbable sutures, vascularization damage, and oxidative stress. Prevention of adhesion formation therefore consists of the prevention of acute inflammation in the peritoneal cavity by means of gentle tissue handling, the addition of more than 5% NO to the CO pneumoperitoneum, cooling the abdomen to 30°C, prevention of desiccation, a short duration of surgery, and, at the end of surgery, meticulous hemostasis, thorough lavage, application of a barrier to injury sites, and administration of dexamethasone. With this combined therapy, nearly adhesion-free surgery can be performed today. Conditioning alone results in some 85% adhesion prevention, barriers alone in 40%-50%.

show abstract

“…Previous comparative study of routine drying method in CO 2 and air drying after hexamethyldisilazane impregnation of rat rectal carcinoma revealed no differences [5].…”

Section: Resultsmentioning

confidence: 99%

Simple Method of Specimen Preparation for Scanning Electron Microscopy

2011

View full text Add to dashboard Cite

We compared conventional method for specimen (cell cultures, tissue specimens) preparation for scanning electron microscopy and a method without sputtering and critical-point drying. OTO-method (osmium-thiocarbohydrazide-osmium) with sample impregnation with hexamethyldisilazane followed by air drying was used as an alternative method. Excellent preservation of surface ultrastructures and electrical conductivity was proved. The method is easy to use and does not require additional costs for equipment.

show abstract

Morphology of the rat peritoneum after carbon dioxide and helium pneumoperitoneum: a scanning electron microscopic study

Cited by 11 publications

References 20 publications

Does thermodynamic stability of peritoneal collagen change during laparoscopic cholecystectomies? A differential scanning calorimetry (DSC) study

Does thermodynamic stability of peritoneal collagen change during laparoscopic cholecystectomies? A differential scanning calorimetry (DSC) study

Role of the peritoneal cavity in the prevention of postoperative adhesions, pain, and fatigue

Simple Method of Specimen Preparation for Scanning Electron Microscopy

Contact Info

Product

Resources

About