Morphology of the basement membrane

Merker, H. J.

doi:10.1002/jemt.1070280203

Cited by 109 publications

(72 citation statements)

References 197 publications

(230 reference statements)

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“…Hence, myofibroblasts could be immunohistochemically distinguished by coexpression of vimentin and a-smooth muscle actin (a-SMA) without expression of desmin or calponin. [4][5][6] Recent investigations have shown that the stromal reaction was more pronounced in Gleason pattern 3 compared to other Gleason pattern tumours. 2,3 To become invasive, prostate cancer cell must first penetrate acinar basement membrane (BM) proteins and extracellular matrix adhesive glycoproteins.…”

Section: Introductionmentioning

confidence: 99%

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Myofibroblastic stromal reaction and expression of tenascin-C and laminin in prostate adenocarcinoma

Tomas

Ulamec

Hudolin

et al. 2006

Prostate Cancer Prostatic Dis

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The aim of this study was to analyse relationship between changes of the stroma and expression of tenascin-C (TN-C) and laminin in prostate carcinoma. Tenascin-C immunostaining was increased, and laminin decreased in carcinomas compared with peritumoural tissue and benign prostate hyperplasia (Po0.05). Statistical analysis confirmed connection between stromal changes and TN-C expression in prostate carcinoma (Po0.05). Gleason pattern 3 carcinomas showed more pronounced stromal reaction and TN-C expression compared with Gleason pattern 4 carcinomas (Po0.05). The main cells in prostate cancer stroma are myofibroblasts that are also responsible for tenascin production. Degradation of laminin was not connected with myofibroblastic stromal changes.

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Section: Introductionmentioning

confidence: 99%

“…5 Structural components of the BM are laminins, type IV and type VII collagens, nidogens and proteoglycans. 6 Laminins are heterotrimeric molecules made up by one a, one b and one g chain. Until today, we know of five a-chains, three b-chains and three g-chains.…”

Section: Introductionmentioning

confidence: 99%

Myofibroblastic stromal reaction and expression of tenascin-C and laminin in prostate adenocarcinoma

Tomas

Ulamec

Hudolin

et al. 2006

Prostate Cancer Prostatic Dis

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“…In addition, endothelium, various types of muscle cell, lipocytes and Schwann cells are surrounded by BM. 1 Structural components of the BM are at least laminins, type IV collagens, nidogens, proteoglycans such as perlecan, fibulins as well as type VII collagen in the BMs containing lamina fibroreticularis layer. Additionally, fibronectin and tenascin are often located to the BM region, but they are not the structural components of the BMs.…”

mentioning

confidence: 99%

Laminin-5 in the progression of carcinomas

Lohi¹

2001

Int. J. Cancer

112

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The basement membrane (BM) separates epithelial elements from the surrounding stroma. BM is dynamic in regulation of epithelial cells differentiation as well as their organization into 3-dimensional tissues. In these functions, among the molecules of the BM, laminins are especially dynamic. Laminins are distributed in a spatially and temporally regulated manner in various epithelial tissues. Various changes in the laminin distribution accompany the malignant transformation of epithelia. The role of the BM and laminins in the progression of carcinomas is not well understood. The BM has been suggested to act as a mechanical barrier against carcinoma cell invasion. BM laminins may play an active role in regulating the migration and proliferation of the carcinoma cells. Laminin isoform laminin-5 expression is typical for some invasive carcinomas and it may act as a ligand for invading carcinoma cells. Neoexpression of laminin-5 has also been associated to proliferative activity of the carcinoma cells. Integrins ␣ 3 ␤ 1 and ␣ 6 ␤ 4 are probable cell surface receptors acting with laminin-5 in the regulation of carcoma cell invasion and proliferation. © 2001 Wiley-Liss, Inc. Key words: laminin-5; carcinomas; invasion; integrin; basement membranesBasement membrane (BM) is a densified sheet of proteins of the extracellular matrix. The BM separates connective tissue from epithelium. In addition, endothelium, various types of muscle cell, lipocytes and Schwann cells are surrounded by BM. 1 Structural components of the BM are at least laminins, type IV collagens, nidogens, proteoglycans such as perlecan, fibulins as well as type VII collagen in the BMs containing lamina fibroreticularis layer. Additionally, fibronectin and tenascin are often located to the BM region, but they are not the structural components of the BMs. BM molecules are produced in cooperation between epithelial and mesenchymal cells. 2 Collagen type IV is a helical, trimeric protein. The most widely expressed type IV collagen trimer is composed of 2 ␣1(IV) chains and one ␣2(IV) chain. 3 The ␣3(IV)-␣6(IV) chains have a more restricted tissue distribution, present for example in the kidney. 4 -6 Nidogen-1 is a 150 kD glycoprotein consisting of three globular domains. 7 It binds collagen IV and laminin networks together. 8 Nidogen-1 binds also perlecan, fibulin-1 and fibulin-2. 9 Type VII collagen forms the anchoring fibrils and connects the BM lamina densa to the underlying connective tissue at least in BMs of stratified epithelium. 10,11 Type VII collagen binds, laminin-5 and type IV collagen. 12 The functions of BM in offering mechanical support are well known. 1 In addition to mechanical functions, the BM influences for cell proliferation, differentiation, adhesion, migration and gene expression. 13,14 BM acts as a factor in maintaining polarity and organization of epithelial cells 1,13,15,16 and it is an active regulator in epithelial-mesenchymal interactions during epithelial cell development. 17 BM molecules can regulate the activity of growth facto...

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“…The lamina reticularis is not apparent in all tissues; however, it is well developed under multilayered epithelium. It is especially pronounced under the respiratory epithelium of large conducting airways, where it may be several microns thick (Merker, 1994). The lamina reticularis is made up of numerous collagen fibrils.…”

mentioning

confidence: 99%

“…The lamina reticularis is made up of numerous collagen fibrils. Immunohistochemical studies have shown that the collagen fibrils consist of Types I, III, V, VI, and VII collagen (Merker, 1994). The collagen fibrils are arranged as a mat of large fibers oriented along the longitudinal axis of the airway when viewed with scanning electron microscopy.…”

mentioning

confidence: 99%

Fibroblast Growth Factor-2 in Remodeling of the Developing Basement Membrane Zone in the Trachea of Infant Rhesus Monkeys Sensitized and Challenged with Allergen

Evans

Winkle

Fanucchi

et al. 2002

Laboratory Investigation

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SUMMARY:Remodeling of the epithelial basement membrane zone (BMZ) involves increased deposition of collagen, resulting in thickening of the BMZ. The current study focuses on fibroblast growth factor-2 (FGF-2) in the tracheal BMZ in house dust mite allergen (HDMA)-sensitized infant rhesus monkeys, challenged with HDMA at a time when the BMZ is undergoing active postnatal development. To detect structural changes in the BMZ, we measured collagens I, III, and V. To detect changes in the function of the BMZ, we measured immunoreactivity of the heparan sulfate proteoglycan, perlecan, and FGF-2. We found significant thickening of the tracheal BMZ (p Ͻ 0.05) with each of these parameters. We also found that all HDMA tracheal samples expressed thin focal areas of the BMZ associated with leukocyte trafficking. These areas were depleted of perlecan and FGF-2; however, increased FGF-2 immunoreactivity was present in the adjacent basal cells. We conclude that basal cells and FGF-2 are involved with significant remodeling of the BMZ in the developing trachea of infant rhesus monkeys exposed to HDMA. (Lab Invest 2002, 82:1747-1754.

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Morphology of the basement membrane

Cited by 109 publications

References 197 publications

Myofibroblastic stromal reaction and expression of tenascin-C and laminin in prostate adenocarcinoma

Myofibroblastic stromal reaction and expression of tenascin-C and laminin in prostate adenocarcinoma

Laminin-5 in the progression of carcinomas

Fibroblast Growth Factor-2 in Remodeling of the Developing Basement Membrane Zone in the Trachea of Infant Rhesus Monkeys Sensitized and Challenged with Allergen

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