Morphology of second and third instars of Chrysomya villeneuvi Patton (Diptera: Calliphoridae), a fly species of forensic importance

Sukontason, Kom; Sukontason, Kabkaew L.; Piangjai, Somsak; Narongchai, Paitoon; Samai, Wirachai; Boonchu, Noppawan; Sripakdee, Duanghatai; Ngern-klun, Radchadawan; Siriwattanarungsee, Sirisuda

doi:10.1016/j.forsciint.2004.10.009

Cited by 17 publications

(11 citation statements)

References 9 publications

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“…The profile of the posterior spiracles in fly puparia used in this study were clearly observed, and well matched with those observed in the third instar, for example, C. megacephala (Zumpt 1965;Ishijima 1967;Sukontason et al 2004), C. nigripes (Sukontason et al 2004), C. rufifacies (Ishijima 1967;Sukontason et al 2004Sukontason et al , 2005b, C. villeneuvi (Sukontason et al 2005a), L. cuprina (Ishijima 1967;Greenberg and Kunich 2002;Sukontason et al 2004), and M. domestica (Ishijima 1967;Goff and Catts 1990;Sukontason et al 2004). Moreover, mean of the range between the posterior spiracles is a useful feature for distinguishing puparia of closely related species (L. cuprina vs H. ligurriens; C. rufifacies vs C.…”

Section: Discussionsupporting

confidence: 86%

Identifying fly puparia by clearing technique: application to forensic entomology

2007

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In forensic investigations, immature stages of the fly (egg, larva, or puparia) can be used as entomological evidence at death scenes, not only to estimate the postmortem interval (PMI), analyze toxic substances, and to determine the manner of death but also to indicate the movement of a corpse in homicide cases. Of these immature stages, puparia represent the longest developmental time, which makes them of useful. However, in order for forensic entomologists to use puparia effectively, it is crucial that they are able to accurately identify the species of fly found in a corpse. Typically, these puparia are similar in general appearance, being coarctate and light brown to dark brown in color, which makes identification difficult. In this study, we report on the clearing technique used to pale the integument of fly puparia, thereby allowing observation of the anterior end (second to fourth segments) and the profile of the posterior spiracle, which are important clues for identification. We used puparia of the blowfly, Chrysomya megacephala (F.), as the model species in this experiment. With placement in a 20% potassium hydroxide solution daily and mounting on a clearing medium (Permount(R), New Jersey), the profile of the posterior spiracle could be clearly examined under a light microscope beginning on the fifth day after pupation, and the number of papillae in the anterior spiracle could be counted easily starting from the ninth day. Comparison of morphological features of C. megacephala puparia with those of other blowflies (Chrysomya nigripes [Aubertin], Chrysomya rufifacies [Macquart], Chrysomya villeneuvi [Patton], Lucilia cuprina [Wiedemann], and Hemipyrellia ligurriens [Wiedemann]) and a housefly (Musca domestica L.) revealed that the anterior ends and the profiles of the posterior spiracles had markedly distinguishing characteristics. Morphometric analysis of the length and width of puparia, along with the length of the gaps between the posterior spiracles of seven fly species, displayed differences among them. This provides a key to identifying the puparia of these seven fly species.

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Section: Discussionsupporting

confidence: 86%

Identifying fly puparia by clearing technique: application to forensic entomology

2007

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“…However, some reports have shown that the morphological characteristics of the posterior spiracle are difficult to use or cannot be used to distinguish between the closely related species. Examples of these were published for blow flies Chrysomya rufifacies (Macquart) vs. Chrysomya villeneuvi Patton (Sukontason et al 2005) or Chrysomya megacephala (Fabricius) vs. Chrysomya pinguis (Walker) (Ishijima 1967).…”

Section: Discussionmentioning

confidence: 99%

“…Delicate spines of L. cuprina and H. ligurriens were similar to other blow flies Lucilia sericata (Meigen), Calliphora spp. (Peters 1992), house fly Musca domestica L., or fly of the family Muscidae, Hydrotaea spinigera Stein (Sukontason et al 2004), but very different from the strong one of blow flies C. megacephala, C. rufifacies, Chrysomya nigripes Aubertin (Sukontason et al 2004), C. villeneuvi (Sukontason et al 2005), Chrysomya bezziana Villeneuve, or Cochliomyia hominivorax (Coquerel) (Peters 1992). Our previous work confirmed the significance of spine features, which are species specific, such as blow flies (C. megacephala, C. rufifacies, C. nigripes) or house fly (Sukontason et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Differentiation between Lucilia cuprina and Hemipyrellia ligurriens (Diptera: Calliphoridae) larvae for use in forensic entomology applications

et al. 2010

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Lucilia cuprina and Hemipyrellia ligurriens are forensically important blow flies in many countries. The immature stage, in particular the third instar, of both species are generally similar in morphological appearance. In this study, the use of three main characteristics, the posterior spiracle, the dorsal spines between the first and second thoracic segments, and the cephalopharyngeal skeleton, allows for identification of both fly species, with the number of papillae on anterior spiracle being a supportive characteristic in some cases. Comparison of these characteristics is illustrated in detail.

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“…The larvae of several species have prominent spiny tubercles not found in other calliphorids (Kitching, 1976;Kitching & Voeten, 1977;Erzinclioglu, 1987;Sukontason et al, 2003Sukontason et al, , 2005 and are sometimes referred to as 'hairy maggots'. All tuberculate species with known natural history are also facultative predators on other maggots [Froggatt, 1918(cited in Fuller, 1934 ;Fuller, 1934;Senior-White et al, 1940;Ullyett, 1950].…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of the blowfly genus Chrysomya

Singh

Kurahashi

Wells

2010

Medical and Veterinary Entomology

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Chrysomya Robineau-Desvoidy (Diptera: Calliphoridae) is a genus of blowfly commonly observed in tropical and subtropical countries of the Old World. Species in this genus are vectors of bacteria, protozoans and helminths, cause myiasis, are predators of other carrion insects, and are important forensic indicators. Hypotheses concerning the evolution of sex determination, larval anatomy and genome size in Chrysomya have been difficult to evaluate because a robust phylogeny of the genus was lacking. Similarly, the monophyly of subgenera was uncertain. The phylogeny of Chrysomya spp. was reconstructed based on 2386 bp of combined mitochondrial cytochrome oxidase subunit I (COI) and nuclear carbamoylphosphate synthetase (CPS) genes. Maximum parsimony (MP), maximum likelihood (ML) and Bayesian analysis (BA) differed only slightly in the resulting tree topology. Chrysomya was monophyletic. Monogenic reproduction is almost certainly derived rather than, as has been suggested, primitive within the genus, and tuberculate larvae probably evolved twice. Genome size is more likely to have decreased over evolutionary time rather than, as has been suggested, increased within the genus, but its correlation with developmental time was not observed. The subgenera Microcalliphora, Eucompsomyia and Achoetandrus were recovered as monophyletic.

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Morphology of second and third instars of Chrysomya villeneuvi Patton (Diptera: Calliphoridae), a fly species of forensic importance

Cited by 17 publications

References 9 publications

Identifying fly puparia by clearing technique: application to forensic entomology

Identifying fly puparia by clearing technique: application to forensic entomology

Differentiation between Lucilia cuprina and Hemipyrellia ligurriens (Diptera: Calliphoridae) larvae for use in forensic entomology applications

Molecular phylogeny of the blowfly genus Chrysomya

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