Morphology of Pseudomonas aeruginosa typing phages of the lindberg set

Ackermann, Hans-W.; Cartier, Christel; Slopek, S; Vieu, J.F.

doi:10.1016/s0769-2617(88)80075-3

Cited by 35 publications

(21 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DNA sequence of this phage showed 90% identity (see Fig. S4 in the supplemental material) with those of the lytic phages 119X and PaP2 (19), defined as one species despite their distant origins in Australia and China, respectively (3,42). Several studies have shown that podoviruses of the KMV species are widely distributed in Europe (10,14,33); however, not a single phage of this species was isolated in this work.…”

Section: Because They Have Different Host Ranges (See Below)mentioning

confidence: 72%

High Diversity and Novel Species of Pseudomonas aeruginosa Bacteriophages

Sepúlveda-Robles

Kameyama

Guarneros

2012

Appl Environ Microbiol

View full text Add to dashboard Cite

ABSTRACTThe diversity ofPseudomonas aeruginosabacteriophages was investigated using a collection of 68 phages isolated from Central Mexico. Most of the phages carried double-stranded DNA (dsDNA) genomes and were classified into 12 species. Comparison of the genomes of selected archetypal phages with extant sequences in GenBank resulted in the identification of six novel species. This finding increased the group diversity by ∼30%. The great diversity of phage species could be related to the ubiquitous nature ofP. aeruginosa.

show abstract

Section: Because They Have Different Host Ranges (See Below)mentioning

confidence: 72%

High Diversity and Novel Species of Pseudomonas aeruginosa Bacteriophages

Sepúlveda-Robles

Kameyama

Guarneros

2012

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Phage YuA was isolated in a pond near Moscow, Russia, and belongs to the Siphoviridae family. Morphological data suggest that it is related to phage M6 from the Lindberg typing set (1,28). Both YuA and M6 are significantly different from other Pseudomonas-infecting siphoviruses deposited in the public databases.…”

mentioning

confidence: 94%

The Genome and Structural Proteome of YuA, a New Pseudomonas aeruginosa Phage Resembling M6

et al. 2008

View full text Add to dashboard Cite

Pseudomonas aeruginosa phage YuA (Siphoviridae) was isolated from a pond near Moscow, Russia. It has an elongated head, encapsulating a circularly permuted genome of 58,663 bp, and a flexible, noncontractile tail, which is terminally and subterminally decorated with short fibers. The YuA genome is neither Mu-nor -like and encodes 78 gene products that cluster in three major regions involved in (i) DNA metabolism and replication, (ii) host interaction, and (iii) phage particle formation and host lysis. At the protein level, YuA displays significant homology with phages M6, JL001, 73, B3, DMS3, and D3112. Eighteen YuA proteins were identified as part of the phage particle by mass spectrometry analysis. Five different bacterial promoters were experimentally identified using a promoter trap assay, three of which have a 54 -specific binding site and regulate transcription in the genome region involved in phage particle formation and host lysis. The dependency of these promoters on the host 54 factor was confirmed by analysis of an rpoN mutant strain of P. aeruginosa PAO1. At the DNA level, YuA is 91% identical to the recently (July 2007) annotated phage M6 of the Lindberg typing set. Despite this level of DNA homology throughout the genome, both phages combined have 15 unique genes that do not occur in the other phage. The genome organization of both phages differs substantially from those of the other known Pseudomonas-infecting Siphoviridae, delineating them as a distinct genus within this family.Despite a decade of major sequencing efforts, many aspects of the genomic diversity among bacteriophages remain to be addressed. Recent metagenomic sequencing of uncultured viral communities from oceanic regions has shown that, although common patterns of genomic organization are present, up to 90% of marine-phage sequences are novel (6, 14). Other phage-sequencing projects revealed distinct levels of genomic diversity among phages infecting different bacteria. For example, the diversity of phage types infecting mycobacteria (37) contrasts sharply with dairy phages, which constitute a closeknit group (15).In recent years, genome sequencing efforts for phages infecting Pseudomonas aeruginosa have revealed this group as strongly diverse at the genome organizational level, which is consistent with their reported diversity in propagation, host interaction, and particle structure. The phages of P. aeruginosa are under investigation to determine the scope of their therapeutic potential and to unravel their dynamic interaction with their pathogenic host. Moreover, insight into the genome content of P. aeruginosa phages allows insight into the evolutionary aspects of these phages. At present, 27 complete genome sequences of phages infecting P. aeruginosa have been deposited in public databases (2). Among the siphoviruses infecting P. aeruginosa, phage D3112 is probably the best studied. With the exception of a DNA modification module and a structural region coding for tail morphogenesis proteins, phage D3112 shares its overall gen...

show abstract

“…Previous classifications of P. aeruginosa phages were mainly based on particle morphology, buoyant density and DNA composition [2,3] and did not include systematic comparison of DNA homology. This paper presents an investigation of DNA homology of 113 temperate and virulent bacteriophages, including the Lindberg typing set phages [3] (some data were already published in Russian [4][5][6]).…”

Section: Introductionmentioning

confidence: 99%

“…This paper presents an investigation of DNA homology of 113 temperate and virulent bacteriophages, including the Lindberg typing set phages [3] (some data were already published in Russian [4][5][6]). …”

Section: Introductionmentioning

confidence: 99%

DNA homology in species of bacteriophages active onPseudomonas aeruginosa

Крылов

Tolmachova

Akhverdian

1993

Archives of Virology

View full text Add to dashboard Cite

Using electron microscopy and DNA-DNA-hybridization, 113 virulent and temperate bacteriophages specific for P. aeruginosa have been assigned to 23 species. In most cases, especially in virulent phages, both particle morphology and DNA homology types were in good correlation and their use was sufficient for clear-cut definition of phage species. No virulent phages of different species had any DNA homology. DNA homology was detected between temperate phages of several species. Temperate phages formed two large groups of two and seven species, respectively. The first group included all transposable bacteriophages. The extent of interspecies DNA homology of phages belonging to each group was not more than 10-15% (except for 25% for phages D 3 and KF 1). No DNA homology was between phages of different groups. The possible origin and function of homologous sequences (genetic modules, linkers, occasional insertional sequences) are discussed. One of the phages (phi C 15) may be considered as the result of recombination between phages belonging to two different species, 295 and SM.

show abstract

Morphology of Pseudomonas aeruginosa typing phages of the lindberg set

Cited by 35 publications

References 14 publications

High Diversity and Novel Species of Pseudomonas aeruginosa Bacteriophages

High Diversity and Novel Species of Pseudomonas aeruginosa Bacteriophages

The Genome and Structural Proteome of YuA, a New Pseudomonas aeruginosa Phage Resembling M6

DNA homology in species of bacteriophages active onPseudomonas aeruginosa

Contact Info

Product

Resources

About