Morphology of Mycoplasma mycoides Thread-phase Growth

Gourlay, R. N.; Thrower, K. J.

doi:10.1099/00221287-54-1-155

Cited by 34 publications

(13 citation statements)

References 2 publications

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“…A capsule staining with RR similar to that demonstrated in the present work has been shown in various mycoplasmas (1,13,14,20,28). It has been suggested that the capsule was associated with the adhesive properties (13,28) or virulence of mycoplasmas (12,14). The relationship between the RR-positive capsule and the radiating fibrils or a fuzzy layer seen on the mycoplasma cells not treated with RR remains to be determined.…”

Section: Discussionsupporting

confidence: 84%

Interaction of Mycoplasma hyopneumoniae with the porcine respiratory epithelium as observed by electron microscopy

Tajima¹,

Yagihashi²

1982

Infect Immun

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An in vivo-passaged strain of Mycoplasina hyopneumoniae attained viability titers of 106 to 108 color-changing units per mg of tissue in pig lungs and caused gross and histological pulmonic lesions. Mycoplasmas were readily located in the lumina of the respiratory tract by electron microscopy. In sections of tissue fixed in glutaraldehyde-osmium, the organisms were found to possess many radial fibrils on the outer surface of the limiting membrane. These fibrils appeared to interconnect adjacent mycoplasmas and to extend between the organism and epithelial cell. Ruthenium red staining demonstrated a thick, dark layer of capsular material enveloping the entire mycoplasma cell. The capsular material was seen to bridge the space between the mycoplasma and host cell. The general morphology of the in vitro-passaged strains grown in broth medium was essentially similar to that of the in vivo-passaged strain. In these organisms, however, no long fibrils were seen, although a fuzzy layer was present outside the cell membrane. The ruthenium red-positive capsule was stained less intensely, and its width was only about one-half that observed for the in vivo-passaged strain. In negatively stained preparations, the cells had an outer fringe of amorphous material apparently corresponding to the fuzzy layer seen in thin sections. The in vitro-passaged strain grew poorly in pig lungs and lost its ability to produce gross pulmonic lesions. The organisms in the respiratory tract had a capsule much thinner than that of the in vivo-passaged strain.

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Section: Discussionsupporting

confidence: 84%

Interaction of Mycoplasma hyopneumoniae with the porcine respiratory epithelium as observed by electron microscopy

Tajima¹,

Yagihashi²

1982

Infect Immun

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“…Capsules have been suggested for several species of Mycoplasma in addition to M. mycoides ssp. mycoides (Gourlay and Thrower, 1968). Examples include Mycoplasma dispar (Almeida and Rosenbusch, 1991), M. gallisepticum (Chu and Horne, 1968), M. hyopneumoniae (Tajima and Yagihashi, 1982), M. penetrans (Neyrolles et al, 1998), M. pneumoniae (Wilson and Collier, 1976) and M. synoviae (Ajufo and Whithear, 1978), based mainly on electron microscopy and cationic staining using ruthenium red (Almeida and Rosenbusch, 1991).…”

Section: Introductionmentioning

confidence: 99%

Identification of exopolysaccharide‐deficient mutants of Mycoplasma pulmonis

Daubenspeck¹,

Bolland²,

Luo

et al. 2009

Molecular Microbiology

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SummaryThe presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal b-linked galactose residues. Mutants that failed to produce the EPS bound by GS-I were isolated from a transposon library. All of the mutants had the transposon located in open reading frame MYPU_7410 or MYPU_7420. These overlapping genes are predicted to code for a heterodimeric pair of ABC transporter permeases and may code for part of a new pathway for synthesis of EPS. Analysis by lectinaffinity chromatography in conjunction with gas chromatography demonstrated that the wild-type mycoplasma produced an EPS (EPS-I) composed of equimolar amounts of glucose and galactose that was lacking in the mutants. Phenotypic analysis revealed that the mutants had an increased propensity to form a biofilm on glass surfaces, colonized mouse lung and trachea efficiently, but had a decreased association with the A549 lung cell line. Confounding the interpretation of these results is the observation that the mutants missing EPS-I had an eightfold overproduction of an apparent second EPS (EPS-II) containing N-acetylglucosamine.

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“…Furthermore, the ultrastructural characteristics of the ibex isolate, evaluated by electron microscopy, showed typical architecture of Mollicutes. Fine granulations were evident on the cell surface, corresponding to a ''slime layer'' made of galactan, known to be present on the surface of Mycoplasma mycoides cluster cells (Gourlay and Thrower, 1968). Despite these indications that the IKC was caused by a Mycoplasma species further work was required to identify it to subspecies level.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Mycoplasma Isolated From an Ibex (Capra Ibex) Suffering From Keratoconjunctivitis in Northern Italy

Giangaspero

Orusa

Nicholas

et al. 2010

Journal of Wildlife Diseases

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ABSTRACT:In 2005 a Mycoplasma species was isolated from ocular-conjunctival swabs from an adult male Alpine ibex (Capra ibex) from the Valle d'Aosta Region, Northern Italy. The animal suffered from bilateral ocular discharge with diffuse inflammation, severe corneal involvement of the left eye and mild corneal opacity of the right eye. Histologic examination revealed a keratoconjunctivitis characterized by lymphocytic and plasmacellular infiltration. Laboratory investigations of the isolate included culture, transmission electron microscopy, PCR, and denaturing gradient gel electrophoresis, as well as DNA sequencing of the 16S rDNA gene. These tests identified the isolate as Mycoplasma mycoides subsp. capri large-colony serovar, an organism that has occasionally been associated with keratoconjunctivitis in goats. For a correct diagnosis, it is necessary to carry out laboratory investigations, as clinical cases of keratoconjunctivitis in wild ruminants are not always ascribable to Mycoplasma conjunctivae.

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Morphology of Mycoplasma mycoides Thread-phase Growth

Cited by 34 publications

References 2 publications

Interaction of Mycoplasma hyopneumoniae with the porcine respiratory epithelium as observed by electron microscopy

Interaction of Mycoplasma hyopneumoniae with the porcine respiratory epithelium as observed by electron microscopy

Identification of exopolysaccharide‐deficient mutants of Mycoplasma pulmonis

Characterization of Mycoplasma Isolated From an Ibex (Capra Ibex) Suffering From Keratoconjunctivitis in Northern Italy

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