Morphology of immature stages of blow fly, Lucilia sinensis Aubertin (Diptera: Calliphoridae), a potential species of forensic importance

Sanit, Sangob; Sukontason, Kom; Kurahashi, Hiroki; Tomberlin, Jeffery K.; Wannasan, Anchalee; Kraisittipanit, Rungroj; Sukontason, Kabkaew L.

doi:10.1016/j.actatropica.2017.09.014

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…Similarly, SEM is utilized to examine the ultrastructure of all other blowfly immature life stages, offering the advantage of appreciating critical characteristics to differentiate closely related species (Sanit et al, 2017). However, light microscopy is more commonly preferred, employing the hydroxide clearing method for larvae (Bunchu et al, 2012) and the potassium hydroxide clearing technique for pupae (K. L. Sukontason et al, 2007).…”

Section: Insect Species Identificationmentioning

confidence: 99%

The Role of Histology in Forensic Investigations

Guareschi,

Kelleher,

Magni

2024

Preprint

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histology, the processing and staining of cells, tissues and organs to highlight contrast, visualize and analyze their microstructure under microscopy, encompasses a vast array of techniques used to confirm, refine, or refute the macroscopic findings in forensic investigations, for example in the case of autopsies. This review presents several applications of histological protocols, staining methods and techniques to case studies, with the aim of illustrating the established, current and potential applications of histological investigations to forensic sciences. The list of disciplines include anthropology, cytology, dendrochronology, diatomology, entomology, fiber analysis, palynology,human and veterinary pathology, taphonomy and thanatology.

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Section: Insect Species Identificationmentioning

confidence: 99%

The Role of Histology in Forensic Investigations

Guareschi,

Kelleher,

Magni

2024

Preprint

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“…The stage of development of immature insects can provide evidence of the time of colonisation, and potential time of death. The examination and age estimation can be improved through electron microscopy examination of the morphology of the instar stages [2]. The developmental period, adjusted for temperature is species dependent and SEM can aid identification of closely related species, for example through ultramicroscopic examination of male genitalia of blowflies [3].…”

Section: Introductionmentioning

confidence: 99%

Microscopy in Forensic Sciences

Jones

2019

Springer Handbooks

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This chapter examines the use of electron microscopy, atomic force microscopy and other analytical techniques in forensic investigation and research. These tools can be used to enhance examination of human remains and trace evidence to improve understanding of cause of death, victim identification or post mortem interval.A police-designed scenario is used to highlight trace evidence such as glass, gun shot residue and paint. The validity of forensic techniques is discussed, with reference to international standards, repeatability, and false convictions. Ballistic evidence is used to highlight the complexities in evidence interpretation, including manufacturing variability, environmental effects and likelihood ratios.The use of SEM, AFM and other techniques in the development of forensic research is showcased, with particular examples from the field of fingerprints. Examples include improvements in the development of fingermarks from difficult surfaces, interaction of evidence types, and added intelligence from the crime scene, such as forensic timeline or gender of perpetrator.

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“…In Thailand, the vast majority of forensic important species investigations have been focused on species identification [ 6 , 7 , 8 ], developmental rate of immature stage [ 9 , 10 ], and insect succession [ 11 , 12 ]. Furthermore, fly surveys have been conducted on a local scale or limited to only specific land use types, for instance, in forest or in mountainous area [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Predicting Geographic Distribution of Forensically Significant Blow Flies of Subfamily Chrysomyinae (Diptera: Calliphoridae) in Northern Thailand

Klong-klaew

Ngoen-Klan

Moophayak

et al. 2018

Insects

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Blow flies (Diptera: Calliphoridae) are carrion-breeding flies that are commonly used as evidence in forensic investigation. An adequate knowledge of ecological and geographical data of blow fly has a direct application in forensic science, as far as estimating time of colonization or corpse relocation. The aim of this study was to evaluate the occurrence of four species of Chrysomyinae (Chrysomya pinguis, Chrysomya chani, Chrysomya villeneuvi, and Ceylonomyia nigripes) across six land use types in central Chiang Mai, northern Thailand. Eighteen study sites were selected for sampling across three districts of Chiang Mai province (Mueang Chiang Mai, Mae Rim, and Hang Dong). Adult flies were collected every two weeks using a funnel trap baited with 1-day tainted beef offal. The predicted geographic distributions of forensically important blow fly species were modeled using the computer program ArcGIS, based on selected climatic variables (temperature, relative humidity, and light intensity) recorded at study sites. During the study period, 1298 adult flies were collected, with peak fly occurrence during summer (April–May). Seasonal fluctuation patterns varied depending on fly species. Climatic factors displayed diverse impact on associated fly populations. Identified species were restricted mainly to mixed deciduous forests (MDF) especially in the mountainous area. None of these flies were trapped in an urban area.

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Morphology of immature stages of blow fly, Lucilia sinensis Aubertin (Diptera: Calliphoridae), a potential species of forensic importance

Cited by 10 publications

References 22 publications

The Role of Histology in Forensic Investigations

The Role of Histology in Forensic Investigations

Microscopy in Forensic Sciences

Predicting Geographic Distribution of Forensically Significant Blow Flies of Subfamily Chrysomyinae (Diptera: Calliphoridae) in Northern Thailand

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