Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method

Yoshida, Daisuke; Yokoyama, Takashi; Shimoaki, Takatsugu; Tomita, Takashi; Yoshida, Tadayoshi; Yamämoto, Yasushi; Taga, Keijiro; Sumino, Ayumi; Dewa, Takehisa; Nango, Mamoru; Yamamoto, Masato; Sheravani, Zameer

doi:10.4236/jbpc.2013.44016

Cited by 14 publications

(32 citation statements)

References 13 publications

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“…The details of the method of monolayer formation have been reported in earlier publi-cation [8]. 1 μl drop of lipids in chloroform solution was dropped gently on the water surface and next was dropped after >1 min so that previous droplet expanded on the water surface.…”

Section: Monolayer Formationmentioning

confidence: 99%

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MorphologicaObservation of Specific Condensation Effect of Cholesterol on Dipalmitoyl Phosphatidyl Choline (DPPC) Monolayer by Dropping Method

Yokoyama¹,

Yoshida²,

Mori³

et al. 2016

JBPC

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Morphology of dipalmitoyl phosphatidyl choline (DPPC)-cholesterol (Chol) mixed monolayer formed on water surface by dropping method was investigated using surface tension measurement (STm), Brewster angle microscopy (BAM), and fluorescence microscopy (FM). STm showed strong condensation effect of Chol in fluidic DPPC monolayer. Excess area (S ex ) from mean mixing state of DPPC and Chol was about twice larger than that by general compression method in the range from x C = 0.2 to 0.4 (x C : mole fraction of Chol). BAM and FM images showed clearly that the fluidic DPPC monolayer changed to condensed rigid monolayer due to the condensation effect of Chol. At more than x C = 0.3 DPPC-Chol mixed monolayer changed to condensed state similar to the Chol monolayer. These results support previous reports by compression method that Chol molecule demonstrates the strong condensation effect to the fluidic monolayer and also indicate that dropping method enables to form unique monolayer on the water surface.

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Section: Monolayer Formationmentioning

confidence: 99%

“…Recently, we have prepared and studied monolayer formation by dropping method [8]. In dropping method, solution droplets are dropped continuously on water surface at regular time interval.…”

Section: Introductionmentioning

confidence: 99%

MorphologicaObservation of Specific Condensation Effect of Cholesterol on Dipalmitoyl Phosphatidyl Choline (DPPC) Monolayer by Dropping Method

Yokoyama¹,

Yoshida²,

Mori³

et al. 2016

JBPC

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“…For the same reason, LE state was observed in DPPC monolayer formation in the early stage of monolayer formation [15].…”

Section: Resultsmentioning

confidence: 70%

“…Details of monolayer formation by dropping method have been reported previously [15]. In brief, 1 mM DHGP-n solution was prepared in chloroform.…”

Section: Monolayer Formationmentioning

confidence: 99%

“…We prepared DHGP-n monolayers on water surface and studied morphology of monolayer using surface tension measurement (STm) and Brewster angle microscopy (BAM). Monolayers were prepared by dropping method [15]. Structural optimization of DHGP-n molecules was performed by density functional theory (DFT) method and results were discussed at molecular level to justify the morphology of these monolayers.…”

Section: Introductionmentioning

confidence: 99%

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Surface Properties of Novel Surfactant, Dihexadecyl Gemini Phosphate, Monolayers on Water Surface by Dropping Method

Kobayashi¹,

Amano²,

Taga³

et al. 2017

JBPC

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We have synthesized first novel dihexadecyl phosphate (DHGP-n) gemini surfactants containing alkyl chain ((C n H 2n ); n = 3, 4, 5, and 6) spacers. Surfactants were used to form monolayers on water surface. Surface tension measurement (STm), Brewster angle microscopy (BAM), and density functional theory (DFT) calculation were used to investigate these monolayers. Surface pressure (π) and molecular area (A); π-A isotherm curves of all DHGP-n gemini surfactants showed a gradual increase in surface pressure without any break points in the curves, confirmed the formation of liquid-expand (LE) type monolayers. BAM observations also supported the STm results. Limited molecular area (A 0 ) of these monolayers depended on the spacer of gemini surfactants without spacer n = 4. Longer alkyl chain spacer in the series had higher value of A 0 in π-A isotherm plots. In the series, A 0 of spacer n = 4 was smallest among A 0 values of other spacers. Density functional theory (DFT) method calculation also confirmed π-A isotherm curves pattern of these surfactants. Calculation showed that both odd and even numbers and length of the alkyl chain spacer influenced the structure of DHGP-n monolayer formation on the water surface.

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Lipid Monolayer and Interaction with Anesthetics

Yamämoto

Taga

2016

Encyclopedia of Biocolloid and Biointerface Science 2V Set

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Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method

Cited by 14 publications

References 13 publications

MorphologicaObservation of Specific Condensation Effect of Cholesterol on Dipalmitoyl Phosphatidyl Choline (DPPC) Monolayer by Dropping Method

MorphologicaObservation of Specific Condensation Effect of Cholesterol on Dipalmitoyl Phosphatidyl Choline (DPPC) Monolayer by Dropping Method

Surface Properties of Novel Surfactant, Dihexadecyl Gemini Phosphate, Monolayers on Water Surface by Dropping Method

Lipid Monolayer and Interaction with Anesthetics

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