Morphology, Molecular Phylogeny and Pathogenicity of Colletotrichum panacicola Causing Anthracnose of Korean Ginseng

Choi, Kyung San; Kim, Wan Gyu; Kim, Hong Gi; Choi, Hyo Won; Lee, Young Kee; Lee, Byung Dae; Lee, Sang Yeob; Hong, Sung Kee

doi:10.5423/ppj.2011.27.1.001

Cited by 25 publications

(29 citation statements)

References 36 publications

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“…Moreover, this analysis also significantly separated C. destructivum, C. higginsianum and C. panacicola from each other with high bootstrap supports based on Bayesian and maximum likelihood analyses, which was indicative of separate species. The results support the findings of Choi et al (2011) who also showed that multilocus phylogeny based on three gene sequences provided sufficient variability to distinguish those closely related species into separate clades.…”

Section: Discussionsupporting

confidence: 88%

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A new anthracnose disease of pyrethrum caused byColletotrichum tanacetisp. nov

et al. 2013

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A new pathogen of pyrethrum (Tanacetum cinerariifolium) causing anthracnose was described as Colletotrichum tanaceti based on morphological characteristics and a four-gene phylogeny consisting of rDNA-ITS, b-tubulin (TUB2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin (ACT) gene sequences. The fungus produced perithecia in culture, requiring an opposite mating type isolate in a heterothallic manner. The initial infection strategy on pyrethrum leaves involved the formation of appressoria followed by production of multilobed infection vesicles in the epidermal cells. Infection and colonization then proceeded through thinner secondary hyphae, which resulted in the initial production of water-soaked lesions followed by black necrotic lesions. The infection process was suggestive of a hemibiotrophic infection strategy. Moreover, phylogenetic analysis clearly showed that C. destructivum, C. higginsianum and C. panacicola were separate species that also had similar intracellular hemibiotrophic infection strategies as C. tanaceti, which all clustered in the C. destructivum complex. Colletotrichum spp. were detected at 1% incidence in seed of 1 of 19 seed lines, indicating the potential for seed as a source of inoculum into crops. Colletotrichum tanaceti was detected in leaf lesions from 11 of 24 pyrethrum fields surveyed between April and July 2012, at a frequency of 1Á3-25Á0% of lesions. Anthracnose probably contributes to the complex of foliar diseases reducing green leaf area in pyrethrum fields in Australia.

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Section: Discussionsupporting

confidence: 88%

“…The results support the findings of Choi et al . () who also showed that multilocus phylogeny based on three gene sequences provided sufficient variability to distinguish those closely related species into separate clades.…”

Section: Discussionmentioning

confidence: 96%

A new anthracnose disease of pyrethrum caused byColletotrichum tanacetisp. nov

et al. 2013

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“…The closest matches with the GAPDH sequence of strain CBS 133701, with 98 % identity (3 nucleotides different), are GenBank GU935850 and GU935851 from C. higginsianum isolates C97027 and C97031 ( Choi et al. 2011 ).…”

Section: Resultsmentioning

confidence: 99%

TheColletotrichum destructivumspecies complex - hemibiotrophic pathogens of forage and field crops

Damm¹,

O’Connell²,

Groenewald³

et al. 2014

Studies in Mycology

180

151

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Colletotrichum destructivum is an important plant pathogen, mainly of forage and grain legumes including clover, alfalfa, cowpea and lentil, but has also been reported as an anthracnose pathogen of many other plants worldwide. Several Colletotrichum isolates, previously reported as closely related to C. destructivum, are known to establish hemibiotrophic infections in different hosts. The inconsistent application of names to those isolates based on outdated species concepts has caused much taxonomic confusion, particularly in the plant pathology literature. A multilocus DNA sequence analysis (ITS, GAPDH, CHS-1, HIS3, ACT, TUB2) of 83 isolates of C. destructivum and related species revealed 16 clades that are recognised as separate species in the C. destructivum complex, which includes C. destructivum, C. fuscum, C. higginsianum, C. lini and C. tabacum. Each of these species is lecto-, epi- or neotypified in this study. Additionally, eight species, namely C. americae-borealis, C. antirrhinicola, C. bryoniicola, C. lentis, C. ocimi, C. pisicola, C. utrechtense and C. vignae are newly described.

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“…Verticillium species (Inderbitzin et al, 2011;Inderbitzin & Subbarao, 2014), Colletotrichum species (Choi et al, 2011), Fusarium species (Zitnick-Anderson et al, 2018), and Phytophthora species (Bilodeau et al, 2014;Martin et al, 2012 were found in high abundance and this represents an interesting avenue for future studies. The honey bee-collected pollen pellets revealed co-occurrences between many of the plant and fungal or oomycete genera.…”

Section: Discussionmentioning

confidence: 99%

High‐resolution biomonitoring of plant pathogens and plant species using metabarcoding of pollen pellet contents collected from a honey bee hive

et al. 2019

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The Canadian beekeeping industry is spread across the country, with the greatest proportion of managed honey bee colonies occurring in the Prairie Provinces. Nationally, the number of beekeepers has recently been trending upwards. Simultaneously, agronomic and environmental plant pest incidents are increasing due to a number of factors, including the introduction of exotic organisms through international trade, which is a major pathway for the introduction of potentially invasive alien species and quarantine pests. Therefore, regulatory agencies are interested in developing high‐throughput tools to achieve earlier detection of unwanted species in order to expedite application of mitigating measures to limit the impacts of their introduction. This study evaluates the potential of pollen pellet contents collected by honey bees to monitor plant pests using metabarcoding, a high‐throughput sequencing (HTS) approach for monitoring complex environmental samples. The study used the ITS1 intergenic region to target oomycetes and fungi, the ATP9‐NAD9 spacer to specifically target Phytophthora species, and the ITS2 region to target plant species. From the HTS results, a number of plants that were detected corresponded to known hosts of certain pathogens or species closely related to potentially invasive plant species. Genera including phytopathogenic species found in the pollen samples comprised Fusarium sp., Ophiostoma sp., Peronospora sp., Phytophthora sp., and Pythium sp. Correlations, high entropy, and co‐occurrences between certain plants and oomycetes or fungi were observed. The potential for using honey bee‐collected pollen pellets to study phytopathogens in a given environment is demonstrated here, and this concept could represent a promising complementary tool for the surveillance of phytopathogens or unwanted plants with previously described air and insect sampling methods if the protocol was applied with additional genetic markers.

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Morphology, Molecular Phylogeny and Pathogenicity of Colletotrichum panacicola Causing Anthracnose of Korean Ginseng

Cited by 25 publications

References 36 publications

A new anthracnose disease of pyrethrum caused byColletotrichum tanacetisp. nov

A new anthracnose disease of pyrethrum caused byColletotrichum tanacetisp. nov

TheColletotrichum destructivumspecies complex - hemibiotrophic pathogens of forage and field crops

High‐resolution biomonitoring of plant pathogens and plant species using metabarcoding of pollen pellet contents collected from a honey bee hive

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