Asiatic pennywort (Centella asiatica L. Urb) is one of the medicinal plants, it had a large spread, especially in tropical and subtropical areas. This study aimed to determine the dose of liquid organic fertilizer (LOF), cattle manure, and their interaction to increase the biomass of asiatic pennywort. This study was conducted at Medan Selayang Sub-district with an altitude ± 25 m ASL from March to May 2022. This study used a factorial randomized block design (RBD), the first factor was the cattle manure: 0; 3.375; 6.75; and 10.125 tons/ha, and the second factor was the LOF application: 0; 1.5; 3; and 4,5 ml/ l. The data were analyzed by an ANOVA, and the mean was continued by a DMRT at P<0.05. The results showed that the application of cattle manure at a dose of 6.75 to 10.125 tons/ha could be increased the number of leaves and the number of primary tendrils by 40.98% and 35.02%, respectively. The LOF-treated at doses of 3 to 4.5 ml/l could be increased petiole length, number of leaves, and number of primary tendrils were 32.94; 60.49; 64.73%. The K0P2 and K3P3 interactions could be increased stalk length by 93.57% respectively.