Morphology, inheritance and mapping of a compressed lamina mutant of carrot

Nothnagel, Thomas; Ahne, R.; Straka, P.

doi:10.1111/j.1439-0523.2005.01154.x

Cited by 7 publications

(4 citation statements)

References 36 publications

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“…Thus, the ovary of cola -mutant flowers was located above the insertion point of the perianth-ring. This trait is always associated with the trait “anther/locule defect” of the cola -type flowers, indicating an irregular structure of several theca and/or locules as was previously shown by histo-morphological investigation (Nothnagel et al, 2005 ). For flower characterization of the mapping population we have used the trait “epigynous/hypogynous” because it is easy to characterize and do not require additional histological analyses.…”

Section: Methodssupporting

confidence: 71%

“…The cola -mutation revealed a compact leaf-habit and a semi-dwarf phenotype that is associated to a reduced cell elongation. Moreover, flowering traits were also affected including an impaired transition to flowering, and abnormal flower architecture with partial male sterility (Nothnagel et al, 2005 , this study). The phenotype was inherited in a monogenic recessive fashion causing hypogynous flowers in homozygous state which could be clearly distinguished from epigynous flowers of the wild-type.…”

Section: Discussionmentioning

confidence: 70%

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Mapping genes governing flower architecture and pollen development in a double mutant population of carrot

Budahn

Barański²,

Grzebelus

et al. 2014

Front. Plant Sci.

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A linkage map of carrot (Daucus carota L.) was developed in order to study reproductive traits. The F2 mapping population derived from an initial cross between a yellow leaf (yel) chlorophyll mutant and a compressed lamina (cola) mutant with unique flower defects of the sporophytic parts of male and female organs. The genetic map has a total length of 781 cM and included 285 loci. The length of the nine linkage groups (LGs) ranged between 65 and 145 cM. All LGs have been anchored to the reference map. The objective of this study was the generation of a well-saturated linkage map of D. carota. Mapping of the cola-locus associated with flower development and fertility was successfully demonstrated. Two MADS-box genes (DcMADS3, DcMADS5) with prominent roles in flowering and reproduction as well as three additional genes (DcAOX2a, DcAOX2b, DcCHS2) with further importance for male reproduction were assigned to different loci that did not co-segregate with the cola-locus.

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Section: Methodssupporting

confidence: 71%

Section: Discussionmentioning

confidence: 70%

Mapping genes governing flower architecture and pollen development in a double mutant population of carrot

Budahn

Barański²,

Grzebelus

et al. 2014

Front. Plant Sci.

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show abstract

“…The cola mutant possesses dwarfish morphology, whereas reduced amount of chlorophyll resulted in yellowish leaves of yellow mutant 54 . The F 2 mapping population (VOM14) of 320 individuals was developed from an initial cross of a homozygous recessive yellow mutant as a female parent and a homozygous recessive cola mutant as pollen parent 54 , 55 . Plants were grown in 19 cm/30 cm w/h plastic pots in a sand/humus mixture (3/1) (v/v) under optimized greenhouse conditions at 25 °C/20 °C (day/night) and 18 h light photoperiod.…”

Section: Methodsmentioning

confidence: 99%

The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases

et al. 2020

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In plants, low molecular weight terpenes produced by terpene synthases (TPS) contribute to multiple ecologically and economically important traits. The present study investigates a carrot terpene synthase gene cluster on chromosome 4 associated with volatile monoterpene production. Two carrot mutants, yellow and cola, which are contrasting in the content of low molecular weight terpenes, were crossed to develop an F2 mapping population. The mapping analysis revealed overlapping QTLs on chromosome 4 for sabinene, α-thujene, α-terpinene, γ-terpinene, terpinen-4-ol and 4-carene. The genomic region of this locus includes a cluster of five terpene synthase genes (DcTPS04, DcTPS26, DcTPS27, DcTPS54 and DcTPS55). DcTPS04 and DcTPS54 displayed genotype- and tissue-specific variation in gene expression. Based on the QTL mapping results and the gene expression patterns, DcTPS04 and DcTPS54 were selected for functional characterization. In vitro enzyme assays showed that DcTPS54 is a single-product enzyme catalysing the formation of sabinene, whereas DcTPS04 is a multiple-product terpene synthase producing α-terpineol as a major product and four additional products including sabinene, β-limonene, β-pinene and myrcene. Furthermore, we developed a functional molecular marker that could discriminate carrot genotypes with different sabinene content in a set of 85 accessions.

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“…For mapping the DcAOX2a gene, the genetically well‐described carrot F 2 population DM19 was chosen. The population was obtained from a cross between the carrot mutants yellow leaf ( YEL ) (Nothnagel and Straka 2003) and compressed lamina ( COLA ) (Nothnagel et al 2005). The F 2 plants (n = 161) were cultivated in 12 cm plastic pots and evaluated under glasshouse conditions.…”

Section: Methodsmentioning

confidence: 99%

Carrot alternative oxidase gene AOX2a demonstrates allelic and genotypic polymorphisms in intron 3

Cardoso

Campos

Costa

et al. 2009

Physiologia Plantarum

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Single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels) are becoming important genetic markers for major crop species. In this study, we focus on variations at genomic level of the Daucus carota L. AOX2a gene. The use of gene-specific primers designed in exon regions on the boundaries of introns permitted to recognize intron length polymorphism (ILP) in intron 3 AOX2a by simple polymerase chain reaction (PCR) assays. The length of intron 3 can vary in individual carrot plants. Thus, allelic variation can be used as a tool to discriminate between single plant genotypes. Using this approach, individual plants from cv. Rotin and from diverse breeding lines and cultivars were identified that showed genetic variability by AOX2a ILPs. Repetitive patterns of intron length variation have been observed which allows grouping of genotypes. Polymorphic and identical PCR fragments revealed underlying high levels of sequence polymorphism. Variability was due to InDel events and intron single nucleotide polymorphisms (ISNPs), with a repetitive deletion in intron 3 affecting a putative pre-miRNA site. The results suggest that high AOX2a gene diversity in D. carota can be explored for the development of functional markers related to agronomic traits.

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Morphology, inheritance and mapping of a compressed lamina mutant of carrot

Cited by 7 publications

References 36 publications

Mapping genes governing flower architecture and pollen development in a double mutant population of carrot

Mapping genes governing flower architecture and pollen development in a double mutant population of carrot

The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases

Carrot alternative oxidase gene AOX2a demonstrates allelic and genotypic polymorphisms in intron 3

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