Morphology engineering of basidiomycetes for improved laccase biosynthesis

Abstract

ObjectiveThis work is the first application of a morphological engineering technique called microparticle-enhanced cultivation (MPEC) aimed at the facilitation of laccase production in the submerged cultures by two basidiomycetes species Cerrena unicolor and Pleurotus sapidus.ResultsThe positive effect of the applied 10 μm Al2O3 microparticles at concentrations from 5 to 30 g Al2O3 l−1 was shown. Laccase activity increased 3.5-fold for C. unicolor and 2-fold for P. sapidus at 15 g Al2O3 l−1 on 9 and 14 day of the cultivation, respectively, compared to the control culture without microparticles. The increase of laccase activity in the cultivation broths was caused by the action of Al2O3 microparticles on the agglomeration of hyphae. It led to the decrease of the size of the pellets, (on average by 2 mm for C. unicolor), the change of their shape (star-shaped pellets for C. unicolor) and the change of their structure (more compact pellets for P. sapidus).ConclusionsApplication of MPEC for the submerged cultures of two laccase-producing basidiomycetes proved successful in increasing of enzyme production.

Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures

“…The second most popular microparticle is aluminum oxide, which proved to be completely insoluble in water and inactive (Etschmann et al 2015 ). Results of other authors confirmed that the most effective aluminum oxide concentration is similar to that of talc, approximately 15–20 g l −1 (Driouch et al 2010a , b ; Etschmann et al 2015 ; Coban et al 2015a ; Antecka et al 2016 ).…”

“…Also for organisms of the genus Aspergilli MPEC led to the increase of product formation: 4-fold higher of α-glucoamylase and β-fructofuranosidase secretion by A. niger (Driouch et al 2010a ), a 3.5-fold higher lovastatin titer produced by A. terreus ATCC 20542 (Gonciarz et al 2016 ) or a 3-fold increase of phytase activity by A. ficuum NRRL 3135 (Coban et al 2015a ). The latest study also proved the positive effect of microparticles for basidiomycetes strains (Antecka et al 2016 ). Despite the fact that fungal morphology for C. unicolor and P. sapidus were completely different, in both cases laccase activity was increased, 3.5 and 2-fold, respectively.…”

“…Murr. 137 and Pleurotus sapidus DSM 8266 (Antecka et al 2016 ) were studied to enhance laccase production by employment of MPEC.…”

“…Finally, rare shapes of pellets called star-shaped pellets (Fig. 2 ) were obtained during cultivation of the basidiomycetes strain C. unicolor in a culture with the addition of 15 g l −1 of aluminum oxide (Antecka et al 2016 ). Surprisingly, another basidiomycetes strain, P. sapidus , changed its morphology in an all together different way after the addition of microparticles, and core–shell pellets, similar to the biomass of A. niger SKAn 1015 after the use of titanate microparticles, were achieved.…”

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Fig. 2 Influence of aluminum oxide microparticles added to the culture medium on C. unicolor morphology: a control run without microparticles, b 15 g l −1 and c 30 g l −1 ; scale-bar size 1 cm; from Antecka et al ( 2016 ) distributed under the terms of the Creative Commons Attribution 4.0 International License

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