Morphology engineering: a new strategy to construct microbial cell factories

Huo, Kaiyue; Zhao, Fengjie; Zhang, Fang; Liu, Ruihua; Yang, Chao

doi:10.1007/s11274-020-02903-5

Cited by 13 publications

(13 citation statements)

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“…Modular pathway engineering is mainly used to rebuild metabolic balance and improve metabolite production. Genome-scale engineering and multi-genome editing are the main engineering strategies at the genome level, while cell-level engineering strategies mainly include transporter engineering, morphological engineering, and consortium engineering (Chen et al 2018 ; Bharathiraja et al 2022 ; Hoek and Borodina 2020 ; Huo et al 2020 ; Duncker et al 2021 ). The production of fatty acid derivatives in microbial cell factories is usually regulated by multiple factors rather than by a single factor.…”

Section: Synthetic Biology Tools For Constructing and Optimizing Micr...mentioning

confidence: 99%

Synthetic biology promotes the capture of CO2 to produce fatty acid derivatives in microbial cell factories

Liu

Luo

et al. 2022

Bioresour. Bioprocess.

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Environmental problems such as greenhouse effect, the consumption of fossil energy, and the increase of human demand for energy are becoming more and more serious, which force researcher to turn their attention to the reduction of CO2 and the development of renewable energy. Unsafety, easy to lead to secondary environmental pollution, cost inefficiency, and other problems limit the development of conventional CO2 capture technology. In recent years, many microorganisms have attracted much attention to capture CO2 and synthesize valuable products directly. Fatty acid derivatives (e.g., fatty acid esters, fatty alcohols, and aliphatic hydrocarbons), which can be used as a kind of environmentally friendly and renewable biofuels, are sustainable substitutes for fossil energy. In this review, conventional CO2 capture techniques pathways, microbial CO2 concentration mechanisms and fixation pathways were introduced. Then, the metabolic pathway and progress of direct production of fatty acid derivatives from CO2 in microbial cell factories were discussed. The synthetic biology means used to design engineering microorganisms and optimize their metabolic pathways were depicted, with final discussion on the potential of optoelectronic–microbial integrated capture and production systems.

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Section: Synthetic Biology Tools For Constructing and Optimizing Micr...mentioning

confidence: 99%

Synthetic biology promotes the capture of CO2 to produce fatty acid derivatives in microbial cell factories

Liu

Luo

et al. 2022

Bioresour. Bioprocess.

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“…The MreB protein forms helical filaments upon GTP or ATP binding oscillating along the cell circumference and pushes the bacterial edges that ultimately define the width and length of rod‐shaped bacteria (Szwedziak & Löwe, 2013 ) (Figure 4A ). Divisome molecular mechanics are orchestrated mainly by the tubulin‐like GTPase FtsZ protein (Silber et al, 2020 ) (Figure 4 ) Meddling with certain elements of these systems derives in i) elongated microbes (FtsZ, its inhibitor MinCD, MreB, SOS‐responsive SulA, PBPs, MreB stabilizer RodZ), ii) spherically shaped bacteria (MreB, PBPs) or iii) reduced cell size owing to increased duplication rates (FtsZ, MinCD) (Huo et al, 2020 ) (Figure 4C ). For instance, in Pseudomonas mendocina NK‐01, the ftsZ gene was overexpressed, fostering cell division and improving mcl ‐PHA yield (Zhao et al, 2019 ).…”

Section: Cofactor and Morphology Engineering Strategiesmentioning

confidence: 99%

“…However, such traditional molecular techniques are inefficient when dealing with essential cytokinesis genes as engineered bacteria exhibit poor growth and altered metabolism (Sperber & Herman, 2017 ; Westfall & Levin, 2018 ). To circumvent these phenotypes, the genetic systems can harbour control switches that enable activation at a given time during fermentation, usually when cells reach a considerable cell density (Batianis et al, 2020 ; Huo et al, 2020 ). In this sense, temperature‐sensitive vectors are valuable tools to compensate for detrimental deletions.…”

Section: Cofactor and Morphology Engineering Strategiesmentioning

confidence: 99%

Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery

Acuña

Poblete‐Castro

2022

Microbial Biotechnology

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Microbial production of biopolymers derived from renewable substrates and waste streams reduces our heavy reliance on petrochemical plastics. One of the most important biodegradable polymers is the family of polyhydroxyalkanoates (PHAs), naturally occurring intracellular polyoxoesters produced for decades by bacterial fermentation of sugars and fatty acids at the industrial scale. Despite the advances, PHA production still suffers from heavy costs associated with carbon substrates and downstream processing to recover the intracellular product, thus restricting market positioning. In recent years, model‐aided metabolic engineering and novel synthetic biology approaches have spurred our understanding of carbon flux partitioning through competing pathways and cellular resource allocation during PHA synthesis, enabling the rational design of superior biopolymer producers and programmable cellular lytic systems. This review describes these attempts to rationally engineering the cellular operation of several microbes to elevate PHA production on specific substrates and waste products. We also delve into genome reduction, morphology, and redox cofactor engineering to boost PHA biosynthesis. Besides, we critically evaluate engineered bacterial strains in various fermentation modes in terms of PHA productivity and the period required for product recovery.

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Section: Introductionmentioning

confidence: 99%

“…Morphology engineering has been demonstrated as a new promising strategy to develop more efficient microbial cell factories to improve bioproduction yield [ 13 , 14 ]. This strategy aims to modify cell shape and division patterns by manipulating genes related to cell morphology [ 14 ]. Cytokinesis is essential for the survival of all cellular organisms as it mediates the separation of mother and daughter cells during cell cycle [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Cytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagataella phaffii

Zhong,

Luo,

Xia

et al. 2024

Microb Cell Fact

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Background Human lysozyme (hLYZ) is a natural antibacterial protein with broad applications in food and pharmaceutical industries. Recombinant production of hLYZ in Komagataella phaffii (K. phaffii) has attracted considerable attention, but there are very limited strategies for its hyper-production in yeast. Results Here through Atmospheric and Room Temperature Plasma (ARTP)-based mutagenesis and transcriptomic analysis, the expression of two genes MYO1 and IQG1 encoding the cytokinesis core proteins was identified downregulated along with higher hLYZ production. Deletion of either gene caused severe cytokinesis defects, but significantly enhanced hLYZ production. The highest hLYZ yield of 1,052,444 ± 23,667 U/mL bioactivity and 4.12 ± 0.11 g/L total protein concentration were obtained after high-density fed-batch fermentation in the Δmyo1 mutant, representing the best production of hLYZ in yeast. Furthermore, O-linked mannose glycans were characterized on this recombinant hLYZ. Conclusions Our work suggests that cytokinesis-based morphology engineering is an effective way to enhance the production of hLYZ in K. phaffii.

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Morphology engineering: a new strategy to construct microbial cell factories

Cited by 13 publications

References 100 publications

Synthetic biology promotes the capture of CO2 to produce fatty acid derivatives in microbial cell factories

Synthetic biology promotes the capture of CO2 to produce fatty acid derivatives in microbial cell factories

Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery

Cytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagataella phaffii

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