Morphology and ultrastructure of trichomes of intact and in vitro plants of stevia rebaudiana Bertoni with reference to biosynthesis and accumulation of steviol glycosides

Бондарев, Н. И.; Sukhanova, M. A.; Semenova, Galina; Goryaeva, O. V.; Andreeva, S. E.; Носов, А. М.

doi:10.3103/s0096392510010037

Cited by 18 publications

(24 citation statements)

References 12 publications

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“…Earlier, we showed that accumulation of DGs occurs largely in green parts of the plant, first of all in leaves and also envelope leaves and stems (Bondarev et al 2004). These findings may argue in favour of that the initial steps of the biosynthesis of DGs take place in chloroplasts, and this important circumstance was noted earlier in our previous works (Bondarev et al 2001(Bondarev et al , 2010Sukhanova et al 2007;Ladygin et al 2008).…”

Section: Introductionmentioning

confidence: 65%

“…The latter by its chemical composition was found to be not identical to the content of plastoglobules, and this circumstance is confirmed by the following observation. Upon fixation of leaves by using only glutaraldehyde without osmium in the course of their preparation for electron microscopy an empty space is formed at the site of plastoglobules as a result of extraction of their content, whereas the content of thylakoids upon using this extraction method is maintained electron-dense (Bondarev et al 2010). In this connection, the electron-dense thylakoids from stevia leave chloroplasts after their investigation by electron microscopy were isolated by using the technique described in the Materials and methods section for its employment for the mass-spectrometric study of their content.…”

Section: Resultsmentioning

confidence: 99%

“…Under the conditions of long day, this plant appears to strongly vegetate but not to transit to flowering (Metivier & Viana 1979). In our previous works, it was shown that namely during such period leaves of stevia contain electron-dense chloroplasts (Sukhanova et al 2007;Ladygin et al 2008;Bondarev et al 2010). In addition, under the same conditions the content of diterpenoid glycosides (DGs) in stevia leaves was considerably higher than that of the flowering plants grown under the conditions of a short day, when similar thylakoids were absent (Bondarev et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Standard samples of kaurenoids (ent-kauren, ent-kaurenol, ent-kaurenal and entkaurenoic acid) were kindly provided by Prof. Lew Mander and Dr. Tomonobu Toyomasu. Transmission electronic microscopy The plant material was prepared for transmission electronic microscopy as described previously (Bondarev et al 2010). …”

Section: Chemicals and Reagentsmentioning

confidence: 99%

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Study of electron-dense thylakoids in chloroplasts of Stevia rebaudiana Bertoni in relation to the biosynthesis of diterpenoids

et al. 2013

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Using transmission electronic microscopy and mass spectrometry electron-dense thylakoids of chloroplasts of Stevia rebaudiana leaves during active vegetable growth of this plant were studied in relation to the biosynthesis of diterpenoid glycosides (DGs). It was found that these compounds are absent in these thylakoids, but they contain a water-insoluble weakly polar ent-kauren, a known biosynthetic precursor of DGs as well as gibberellins. This finding provides a base for the suggestion that similar, electron-dense, thylakoids were observed earlier by other authors in other plant species. These data allowed us to conclude that an intensive biosynthesis of ent-kauren is likely related to adaptation of the short-day plants including Stevia rebaudiana to vegetable growth under the long day conditions.

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Section: Introductionmentioning

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Chemicals and Reagentsmentioning

confidence: 99%

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Study of electron-dense thylakoids in chloroplasts of Stevia rebaudiana Bertoni in relation to the biosynthesis of diterpenoids

et al. 2013

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“…However, contrary to earlier report (Yadav et al 2013), the tetraploid plants had approximately 2-4 times more glands than the diploid controls (p<0.05, Table 3). The glandular trichomes had been demonstrated that their apical part of the gland exposed above the leaf surface had subcuticular cavity which can be filled with secrete, and a positive correlation between glands' density and steviol glycosides content (Bondarev et al 2010). Tetraploid plants also had a significantly higher chlorophyll content index than the diploid controls (p<0.05, Table 4), indicating that the former have higher photosynthetic capacity.…”

Section: Colchicine Treatmentmentioning

confidence: 99%

Induction, identification and characterization of polyploidy in <i>Stevia rebaudiana</i> Bertoni

Hong

Juan

et al. 2018

Plant Biotechnology

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Stevia rebaudiana Bertoni produces sweet steviol glycosides; these are regarded as the most promising substitute for sucrose and have a wide range of applications with high economic value. To improve steviol glycosides yields, we established an efficient method for inducing polyploidy in S. rebaudiana. Treatment with 0.05% colchicine for 48 h or with 0.1% colchicine for 24 h efficiently induced polyploidy in germinating seeds and polyploidy could be accurately identified using flow cytometry. Most of the tetraploid S. rebaudiana plants exhibited apparent variations in cytological and morphological characteristics, and had higher contents of the two main steviol glycosides, stevioside and rebaudioside A, than the diploid controls. These tetraploid plants may be further selected for breeding purposes or micropropagated for commercial production. Stevia rebaudiana Bertoni is an herbaceous perennial plant of the Asteraceae family. It is native to the Amambay region of northeastern Paraguay and neighboring countries Brazil and Argentina (Madan et al. 2010;Yadav et al. 2011). Its leaf and stem tissues accumulate a mixture of more than 20 different glycosides derived from the tetracyclic diterpene steviol (Ohta et al. 2010). The main steviol glycosides are stevioside and rebaudioside A, which are the sweetest compounds that are 250-300 and 350-450 times sweeter than sucrose, respectively (Crammer and Ikan 1986). Because the steviol glycosides are naturally occurring compounds with high sweetness and little to no calorific value, they have been regarded as the most promising substitutes for sucrose and have a wide range of applications and high economic value (Soejarto et al. 1983;Yadav et al. 2011). Applications include dietary, diabetic, and dental consumer markets, and health-promoting uses as antitumor, antihypertensive, antihyperglycemic and antioxidant agents (Singh et al. 2015). S. rebaudiana has been introduced and is grown as a crop in a number of countries, including Japan, China, Korea, Indonesia, Tanzania, Mexico, the United States and Canada (Madan et al. 2010).To improve steviol glycosides yields in S. rebaudiana, polyploidy breeding may be a promising approach.Polyploidy is widely acknowledged as a major mechanism of adaptation and speciation in the evolution of higher plants (Ramsey and Schemske 1998). Polyploid plants can arise spontaneously in nature by several mechanisms, including meiotic or mitotic failures and fusion of unreduced (2n) gametes (Comai 2005). In general, polyploid plants are large, healthy and vigorous. Some chemicals induce polyploidy in plants and cell cultures; the best known of these chemicals is colchicine, which frequently causes chromosome doubling (Blakeslee and Avery 1937). Polyploidy has been used to develop new crop cultivars; including wheat, cotton, potato and sugarcane.The primary objectives of this study were to establish a protocol for induction of polyploidy in S. rebaudiana. Seeds of S. rebaudiana cultivar Zhongshan No. 2 were germinated by placing them on p...

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Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

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Morphology and ultrastructure of trichomes of intact and in vitro plants of stevia rebaudiana Bertoni with reference to biosynthesis and accumulation of steviol glycosides

Cited by 18 publications

References 12 publications

Study of electron-dense thylakoids in chloroplasts of Stevia rebaudiana Bertoni in relation to the biosynthesis of diterpenoids

Study of electron-dense thylakoids in chloroplasts of Stevia rebaudiana Bertoni in relation to the biosynthesis of diterpenoids

Induction, identification and characterization of polyploidy in <i>Stevia rebaudiana</i> Bertoni

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

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