Morphology and serotonergic innervation of physiologically identified cells of the guinea pig's myenteric plexus

Erde, SM; Sherman, Diane L.; Gershon,

doi:10.1523/jneurosci.05-03-00617.1985

Cited by 116 publications

(75 citation statements)

References 41 publications

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“…The other AH neurons are also presumed not to supply the muscle. AH neurons receive morphologically identifiable synapses (Erde et al, 1985;Llewellyn-Smith et al, 1988a;Pompolo and Fumess, 1988), but this study indicates that few, if any, of these are likely to be SPimmunoreactive. However, each AH cell undoubtedly receives a large number of ultrastructurally unspecialized contacts from SP-positive vesiculated nerve fiber profiles, since this is the case for all myenteric neurons.…”

Section: Vesicles In Sp-immunoreactive Nerve Fibersmentioning

confidence: 62%

“…Between 10 and 35% of myenteric neurons belong to this group (Furness and Costa, 1987). These neurons have large, smooth cell bodies (Dogiel Type II morphology), are usually located centrally within the ganglia, and often give rise to several long processes (Bomstein et al, 1984a;Erde et al, 1985;Iyer et al, 1988). About 85% of AH neurons are immunoreactive for calcium binding protein (calbindin D 28K) and do not provide terminals to the muscle (Furness et al, 1988a;Iyer et al, 1988).…”

Section: Vesicles In Sp-immunoreactive Nerve Fibersmentioning

confidence: 99%

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Ultrastructural analysis of substance P-immunoreactive nerve fibers in myenteric ganglia of guinea pig small intestine

1989

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Electron microscopic immunocytochemistry has been used to study the distribution and synaptic relationships of substance P (SP)- immunoreactive nerve fibers in ultrathin sections from whole-mount preparations of myenteric ganglia from guinea pig small intestine. At the light microscopic level, myenteric ganglia stained for ultrastructural study contained dense arrays of SP-immunoreactive nerve fibers around and between the neuronal and glial cell bodies. At the electron microscopic level, SP-containing nerve fiber profiles occurred throughout the neuropil of the myenteric ganglia. Both vesicle- containing and nonvesiculated nerve fiber profiles were immunoreactive. The positive vesiculated profiles contained variable proportions of small clear and large granular vesicles. Two-thirds of the vesicle- containing nerve fiber profiles in myenteric ganglia were immunoreactive for SP. Many vesiculated SP-immunoreactive nerve fiber profiles were directly apposed to each myenteric neuron. However, only about 0.6% of these vesiculated profiles formed synapses that showed the ultrastructural specializations of vesicle clustering presynaptically and fuzzy electron-dense material on the postsynaptic membrane. On the other hand, morphologically identifiable synapses with SP-immunoreactivity comprised about half the total number of synapses in the ganglia. SP-immunoreactive synapses were observed on nonvesiculated nerve processes in the periphery of the ganglia and on nerve cell bodies. Most of the axosomatic SP synapses occurred on small neurons that lay either on the surfaces of the ganglia or near the fibers of the internodal strands where they traveled through the ganglia. Both SP-positive and SP-negative nerve cell bodies received SP synapses.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Vesicles In Sp-immunoreactive Nerve Fibersmentioning

confidence: 62%

Section: Vesicles In Sp-immunoreactive Nerve Fibersmentioning

confidence: 99%

Ultrastructural analysis of substance P-immunoreactive nerve fibers in myenteric ganglia of guinea pig small intestine

1989

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“…Similar to CNS astrocytes, many EGC processes display small swollen end-feet that make contact with the basal lamina that surrounds the ganglia or with neighboring blood vessels (Erde et al, 1985;Hanani and Reichenbach, 1994). Two different morphologies have been ascribed to EGC depending on their location within the plexus.…”

Section: Localization and Phenotypic Characteristics Of Egcmentioning

confidence: 99%

Role of enteric glial cells in inflammatory bowel disease

Cabarrocas

Savidge

Liblau

2002

Glia

159

154

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Enteric glial cells (EGCs) represent an extensive but relatively poorly described cell population within the gastrointestinal tract. Accumulating data suggest that EGCs represent the morphological and functional equivalent of CNS astrocytes within the enteric nervous system (ENS). The EGC network has trophic and protective functions toward enteric neurons and is fully implicated in the integration and the modulation of neuronal activities. Moreover, EGCs seem to be active elements of the ENS during intestinal inflammatory and immune responses, sharing with astrocytes the ability to act as antigen-presenting cells and interacting with the mucosal immune system via the expression of cytokines and cytokine receptors. Transgenic mouse systems have demonstrated that specific ablation of EGC by chemical ablation or autoimmune T-cell targeting induces an intestinal pathology that shows similarities to the early intestinal immunopathology of Crohn's disease. EGCs may also share with astrocytes the ability to regulate tissue integrity, thereby postulating that similar interactions to those observed for the blood-brain barrier may also be partly responsible for regulating mucosal and vascular permeability in the gastrointestinal tract. Disruption of the EGC network in Crohn's disease patients may represent one possible cause for the enhanced mucosal permeability state and vascular dysfunction that are thought to favor mucosal inflammation. GLIA 41:81-93, 2003.

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“…Those few ChAT or tachykinin-IR cells that were apposed by 5-HT-IR boutons were not prominent. Synaptology and thus the function of serotoninergic fibers emerging from serotoninergic descending interneurons has not been clarified yet since the reports that investigated the problem published different results (Erde et al, 1985;Young and Furness, 1995;Neal and Bornstein, 2007). Erde et al (1985) reported that mainly type II/AH neurons considered sensory neurons were contacted by serotoninergic terminals.…”

Section: A Possible Enteric Circuit Underlying Serotonin-tachykinin Imentioning

confidence: 99%

“…Synaptology and thus the function of serotoninergic fibers emerging from serotoninergic descending interneurons has not been clarified yet since the reports that investigated the problem published different results (Erde et al, 1985;Young and Furness, 1995;Neal and Bornstein, 2007). Erde et al (1985) reported that mainly type II/AH neurons considered sensory neurons were contacted by serotoninergic terminals. According to Young and Furness (1995), Dogiel type II neurons received a few serotonin inputs and none of them formed synapses, whereas the highest number of serotonin terminals were related to Dogiel type I neurons.…”

Section: A Possible Enteric Circuit Underlying Serotonin-tachykinin Imentioning

confidence: 99%

Interactions of Serotoninergic, Cholinergic, and Tachykinin‐Containing Nerve Elements in the Rabbit Small Intestine

Denes

Wilhelm

NÉMeth

et al. 2009

The Anatomical Record

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This report presents novel results on the effects of serotonin (5-HT) on longitudinal muscle contractions in the rabbit ileum and the interactions of serotonin with some neuronal elements of the myenteric plexus. We showed previously that serotonin-triggered contractions involved two mechanisms in the rabbit ileum: neuronal excitation (via 5-HT 2 receptors in the neurons) and direct muscular stimulation (via 5-HT 4 receptors in the muscle). Here, we focus on the neuronal 5-HT 2 receptor pathway and report further pharmacological and immunocytochemical data clarifying the details of the mechanisms. We observed that antagonists for neurokinin (NK1 and NK2) receptors partially blocked the serotonin response, but NK3 receptor antagonists had no effect. Pretreatment by atropine (ATR) eliminated the NK1 receptor antagonist resistant contractions. In contrast, the NK1 antagonist did not depress the ATR-resistant contraction when ATR was added first. 5-HT 2 receptor agonist-induced contractions were partially suppressed by ATR, hexamethonium, and NK1 or NK2 receptor antagonists. In conclusion, serotonin acting through 5-HT 2 receptors could stimulate interneurons and excitatory motor neurons. Immunocytochemical staining revealed an extensive tachykinin-immunoreactive (IR) network in the myenteric plexus. Approximately 52% of all myenteric neurons were labeled. 5-HT-IR fibers could be detected around both choline acetyltransferase-and tachykinin-IR cells, suggesting functional relationships between them. Consistent with our pharmacological observations, we found that immunopositive nerve elements for 5-HT 2A receptor and double-labeled immunostaining revealed a remarkable overlap between tachykinin-IR neurons and 5-HT 2A -IR elements. Anat Rec, 292:1548Rec, 292: -1558Rec, 292: , 2009. Key words: ileum; 5-HT; tachykinins; 5-HT 2 receptors; rabbit One of the principal regulators of digestion in mammals is the enteric nervous system. Enteric neurons coordinate a variety of functions, such as the secretion of digestive enzymes, control of absorption, and the regulation of motility. Intestinal smooth muscle contractility is organized by polarized reflex pathways through both excitatory and inhibitory neurons (Goyal and Hirano, 1996;Kunze and Furness, 1999;Olsson and Holmgren,

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Morphology and serotonergic innervation of physiologically identified cells of the guinea pig's myenteric plexus

Cited by 116 publications

References 41 publications

Ultrastructural analysis of substance P-immunoreactive nerve fibers in myenteric ganglia of guinea pig small intestine

Ultrastructural analysis of substance P-immunoreactive nerve fibers in myenteric ganglia of guinea pig small intestine

Role of enteric glial cells in inflammatory bowel disease

Interactions of Serotoninergic, Cholinergic, and Tachykinin‐Containing Nerve Elements in the Rabbit Small Intestine

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