Morphology and Nucleic Acid of Rice Gall Dwarf Virus

Boccardo, G.; Milne, Robert G.; Disthaporn, S.; Chettanachit, D.; Putta, M.

doi:10.1159/000149600

Cited by 24 publications

(10 citation statements)

References 14 publications

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“…Sedi mentation was from left to right (ar row). from 0.5 x 10h to 2.7 x 106 [Omura et al, 1983;Kawano et al, 1984;Boccardo and Milne, 1985], We have reported that RRSV particles have polymerase activity [Uyeda et al, 1987], and we now describe the characteristics and products of this enzyme.…”

Section: Introductionmentioning

confidence: 66%

“…4). The Mr o f the RNAs, using tobacco mosaic virus (2.0-2.1 x 106), MS-2 phage (1.24 x 106), Escherichia coli 23S (1.0 x I04) and 16S (0.53 x 106) RNAs as stan dards, were estimated to be about half (or slightly larger) of those reported for the genomic dsRNAs [Kawano et al, 1984;Boccardo and Milne, 1985]. The 32P-labeled RNA segments 1-4, 7 and 8, and 9 and 10 were not resolved and appeared as single bands.…”

Section: Analysis O F the Rnas Synthesized In Vitromentioning

confidence: 99%

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Characterization of RNA Polymerase Associated with Rice Ragged Stunt Virus

1987

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The RNA polymerase associated with rice ragged stunt virus (RRSV) was characterized. Activity was optimum at 35–40° in 0.1 MTris-HCl (pH 8.5) and 6–8 mMMgCl₂. S-Adenosyl-L-methionine stimulated the activity about 5- to 6-fold. It was also stimulated in the presence of chymotrypsin (200 µg/ml). The molecular weights of RNAs synthesized in vitro were calculated to be about half those of the respective genome segments. The synthesized RNAs hybridized to the genome RNAs, and the hybrids migrated identically to the genome RNAs in PAGE. These results indicate that RRSV particles transcribe full-length copies of the genome RNAs. The characteristics of the polymerase are discussed in relation to those of other members of the Reoviridae.

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Section: Introductionmentioning

confidence: 66%

Section: Analysis O F the Rnas Synthesized In Vitromentioning

confidence: 99%

Characterization of RNA Polymerase Associated with Rice Ragged Stunt Virus

1987

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“…Genomic dsRNAs from rice gall dwarf virus (RGDV), rice dwarf virus (RDV) (both of subgroup I), MRDV and Fiji disease virus (FDV) (both of subgroup II), and rice ragged stunt virus (RRSV) (subgroup liD, all from the collection of this Institute (Boccardo et at., 1985), were denatured and electrophoresed by the same method. RNAs were then transferred overnight to nitrocellulose membranes, and treated as above for dot blots.…”

Section: Methodsmentioning

confidence: 99%

In vitro transcription of the double-stranded RNA genome of maize rough dwarf virus (Reoviridae)

Marzachı̀

Accotto

d'Aquilio

et al. 1990

Journal of General Virology

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An RNA-dependent RNA polymerase associated with particles of maize rough dwarf virus, a Fijivirus, was characterized using two in vitro assays differing in their energy regeneration systems. Optimum reaction rates occurred at pH 8-0 to 8.5 at 20 *C. The presence of virus and Mn 2+ or Mg 2+ was essential for enzyme activity; Mn 2+ stimulated more incorporation events than Mg z+, at optimum concentrations of 2 to 4 mM and 4 mM, respectively. Incorporation was not affected by ~-amanitin, actinomycin D or rifampicin. The products synthesized in vitro were single-stranded RNAs which hybridized specifically with the doublestranded genomic RNAs of the template virus, but not with genomic RNAs of five other reoviruses. The in vitro transcripts were also used to detect maize rough dwarf virus RNA in plants and in vector insects.

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“…The molecular sizes cal culated for RRSV RNAs ranged from 0.60 x 10fc to 2.98 x 106 daltons, with a total of about 18.15 x 106 daltons. For RRSV, values of the genome size ranging from 11.63 x 106 to 22.91 x 106 have been reported [7,10,13,23,24].…”

Section: Segmentation O F the Viral Genomementioning

confidence: 99%

Comparison of Proteins and Nucleic Acids of Echinochloa Ragged Stunt and Rice Ragged Stunt Viruses

Chen¹,

Hsu²,

Chen

et al. 1989

Intervirology

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Echinochloa ragged stunt virus (ERSV) and rice ragged stunt virus (RRSV; Reoviridae) were purified from their respective host plants and disrupted in SDS. Evidence for the double-strandedness of ERSV RNA was obtained. Electrophoresis in 10% polyacryl-amide gels resolved the RNAs of each virus into 10 segments, ranging for ERSV from 0.61 × 10⁶ to 2.8 × 10⁶ daltons, with a sum representing the total genome of 17.89 × 10⁶ daltons, and for RRSV from 0.6 × 10⁶ to 2.98 × 10⁶ daltons, with a sum of 18.15 × 10⁶ daltons. Although the overall RNA pattern of the two viruses was similar, there were distinctive features. Using 7.5–15% polyacrylamide gels, electrophoresis of SDS-dissociated RRSV particles yielded five major proteins of 129 × 10³,123 × 10³, 63 × 10³ and 35 × 10³ daltons, and two minor proteins of 113 × 10³ and 88 × 10³ daltons, respectively, while comparable samples of ERSV yielded four major proteins of 127 × 10³,123 × 10³,63 × 10³ and 34 × 10³ daltons, and three minor proteins of 103 × 10³,50 × 10³ and 49 × 10³ daltons, respectively. We conclude from this and other evidence that ERSV and RRSV are closely related viruses and should be classified in the same subgroup, despite reported differences in the morphology of their outer capsids.

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Morphology and Nucleic Acid of Rice Gall Dwarf Virus

Cited by 24 publications

References 14 publications

Characterization of RNA Polymerase Associated with Rice Ragged Stunt Virus

Characterization of RNA Polymerase Associated with Rice Ragged Stunt Virus

In vitro transcription of the double-stranded RNA genome of maize rough dwarf virus (Reoviridae)

Comparison of Proteins and Nucleic Acids of Echinochloa Ragged Stunt and Rice Ragged Stunt Viruses

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