Morphology and Multi-Gene Phylogeny Reveal Pestalotiopsis pinicola sp. nov. and a New Host Record of Cladosporium anthropophilum from Edible Pine (Pinus armandii) Seeds in Yunnan Province, China

Tibpromma, Saowaluck; Mortimer, Peter E.; Karunarathna, Samantha C.; Zhan, Fangdong; Xu, Jianchu; Promputtha, Itthayakorn; Yan, Kai

doi:10.3390/pathogens8040285

Cited by 16 publications

(10 citation statements)

References 55 publications

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“…Parameters for the GTR + I + G model of the combined ITS, TEF1-α and TUB2 were as follows: Estimated base frequencies; A = 0.246189, C = 0.263688, G = 0.243646, T = 0.246477; substitution rates AC = 1.335541, AG = 3.561498, AT = 1.209470, CG = 1.017519, CT = 5.175761, GT = 1.000000; gamma distribution shape parameter α = 0.763268. The phylogenetic tree obtained in this study showed similar results to previous studies (Tibpromma et al 2019 ). The maximum parsimonious dataset consisted of which 924 constants, 395 (42.74%) parsimony-informative and 173 parsimony-uninformative characters.…”

Section: Resultssupporting

confidence: 91%

Novel species of Pestalotiopsis fungi on Dracaena from Thailand

et al. 2020

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A survey of the diversity and distribution of microfungi on Dracaena leaf litter in Songkhla Province (Thailand) yielded two collections of pestalotiopsis-like fungi. Analyses of a combined ITS, TEF1-α and TUB2 sequence data matrix were applied to infer the phylogenetic position of these new isolates in Pestalotiopsis . The phylogenies indicated that these two isolates were monophyletic and constituted a distinct lineage that perceived a taxonomic novelty in Pestalotiopsis . This clade shared a close phylogenetic affinity with P. adusta, P. krabiensis, P. pandanicola and P. papuana . The comparison of morphological features with the phylogenetically closely related taxa are given and the new species is introduced as Pestalotiopsis dracaenicola sp. nov. with comprehensive descriptions and illustrations herein.

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Section: Resultssupporting

confidence: 91%

Novel species of Pestalotiopsis fungi on Dracaena from Thailand

et al. 2020

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“…The cultures were placed at 28°C in an incubator. Observation of fungal morphological structures followed Wagner et al [14], Sandoval-denis et al [15], and Tibpromma et al [16]. The pure cultures ( Figure 2, Supplementary Table 1) were deposited at the Kunming Institute of Botany Culture Collection (KMUCC), while the herbaria of new species were deposited at the Key Laboratory of Industrial Microbiology and Fermentation Technology of Yunnan (YMF), Kunming, Yunnan Province, China.…”

Section: Sample Collection Specimen Examination and Isolationmentioning

confidence: 99%

Discovery of novel fungal species and pathogens on bat carcasses in a cave in Yunnan Province, China

Karunarathna

Dong

Karasaki

et al. 2020

Emerging Microbes & Infections

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Virulent infectious fungal diseases, in natural and managed landscapes, are increasing. Fungal diseases in humans, animals and plants have caused die-off and extinction events and have become a threat to food security. A caving expedition in Yunnan Province, China, revealed two bat carcasses covered in fungal mycelia. Eleven fungal isolates were obtained from these bat carcasses, and morphological observations and multigene phylogenetic analyses revealed they were Fusarium incarnatum, Mucor hiemalis and Trichoderma harzianum and four new species, Mortierella rhinolophicola, M. multispora, M. yunnanensis and Neocosmospora pallidimors. One of the more alarming findings is that a number of infections related to Neocosmospora, previously associated with human and animal mycotoxicoses, are reported to be increasing, and here we present a new species from this genus, isolated from dead bats. Due to the ecosystem services provided by bats, and the close relationship between bats and humans, future research should focus on the impacts and significance of N. pallidimors to human and animal health, examining its pathogenicity and secondary metabolites. Taxonomic descriptions, color images of the habitat, in situ samples, microstructures and cultures are presented. SEM photographs of microstructures and phylogenetic trees showing the placement of new and known species are also provided.

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“…Norphanphoun et al (2017) combined morphological characteristics and multi-gene loci (ITS, large nuclear ribosomal RNA subunit sequence (LSU), RNA polymerase II subunit (RPB2), and α-actin (ACT)) for determination and phylogenetic analysis of the Cytospora species. Furthermore, Tibpromma et al (2019) reported usage of morphological examinations, culture characteristics and multi-gene sequence analysis (ITS, ACT, and translation elongation factor 1-alpha gene (TEF1)) for the determination of fungi isolates belonging to the genera Cladosporium. These scientific works suggested that the Cytospora sp.…”

Section: Discussionmentioning

confidence: 99%

DNA Barcoding of Fungi in the Forest Ecosystem of the Psunj and Papuk Mountains in Croatia

Ćelepirović

Agbaba

Vlahović

2020

SEEFOR

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The saprotrophic, endophytic, and parasitic fungi were detected from the samples collected in the forest of the management unit East Psunj and Papuk Nature Park in Croatia. The disease symptoms, the morphology of fruiting bodies and fungal culture, and DNA barcoding were combined for determining the fungi at the genus or species level. DNA barcoding is a standardized and automated identification of species based on recognition of highly variable DNA sequences. DNA barcoding has a wide application in the diagnostic purpose of fungi in biological specimens. DNA samples for DNA barcoding were isolated from infected tree tissues, fungal fruiting bodies or fungal cultures. The ITS or ITS2 sequences of the fungal DNA were sequenced and aligned with the reference sequences in GenBank (NCBI) using BLAST. The size of ITS and ITS2 sequences were 512-584 bp and 248-326 bp, respectively. The sequences showed a high identity of 97.21%-100% at 98%-100% coverage with reference sequences in GenBank (NCBI). The exception was the species Amphilogia gyrosa that showed 95.65% identity at 100% coverage. Two fungi were determined at genus level: Cladosporium sp., and Cytospora sp., while 11 fungi were determined at species level: Alternaria alternata, Aureobasidium pullulans, Amphilogia gyrosa, Capronia pilosella, Cryphonectria parasitica, Exidia glandulosa, Epicoccum nigrum, Penicillium glabrum, Pezicula carpinea, Rosellinia corticium, and Stereum hirsutum.

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Morphology and Multi-Gene Phylogeny Reveal Pestalotiopsis pinicola sp. nov. and a New Host Record of Cladosporium anthropophilum from Edible Pine (Pinus armandii) Seeds in Yunnan Province, China

Cited by 16 publications

References 55 publications

Novel species of Pestalotiopsis fungi on Dracaena from Thailand

Novel species of Pestalotiopsis fungi on Dracaena from Thailand

Discovery of novel fungal species and pathogens on bat carcasses in a cave in Yunnan Province, China

DNA Barcoding of Fungi in the Forest Ecosystem of the Psunj and Papuk Mountains in Croatia

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