Morphology and molecular study of Fascioloides magna – a growing threat to cervids (Cervidae) in Poland

Houszka, M; Piekarska, Jolanta; Podkowik, Magdalena; Gorczykowski, Michał; Bania, Jacek

doi:10.1515/jvetres-2016-0065

Cited by 16 publications

(27 citation statements)

References 15 publications

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“…Kolejne badania sekcyjne i molekularne zwierząt pochodzących z południowo--zachodniej Polski (z okolic Jawora i Nowogrodu Bobrzańskiego) wykazały inwazję F. magna u 13,6% saren, 70% jeleni i 33,3% danieli. Badaniami koproskopowymi wykryto u jeleni i danieli jaja pasożyta, natomiast w kale sarn jaj nie stwierdzono (12). Obecność F. magna była już wcześniej zarejestrowana u jeleni, sarn i danieli w okolicach Nowogrodu Bobrzańskiego, który jest siedzibą Nadleśnictwa Krzystkowice, obejmującego Bory Zielonogórskie (3), natomiast stwierdzenie inwazji przywry w Nadleśnictwie Jawor jest rozszerzeniem ogniska fascioloidozy o około 80 km w kierunku południowo-wschodnim (12).…”

Section: Materiał I Metodyunclassified

Fascioloides magna: a parasite of roe deer in Bory Zielonogórskie

Demiaszkiewicz¹,

Kowalczyk²,

Filip³

et al. 2018

Medycyna Weterynaryjna

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The aim of this study was to determine the prevalence of infection with F. magna flukes in roe deer from the conifer forest Bory Zielonogórskie. Parasitological dissection was performed on 20 roe deer hunted in May 2016. Faecal samples from the animals were examined by the decantation method. One roebuck was verified to be infected with F. magna, which yielded a prevalence of 5%. We noted fibrinous deposits on the diaphragmatic surface of the liver with adhesions to the diaphragm, as well as five dark-coloured nodular protrusions on the liver surface. In the cross section view of the infected liver, we saw five pseudocysts corresponding to the nodular protrusions observed on the liver surface. Three of the pseudocysts were filled with dark brown liquid containing a few flukes, their eggs and cell detritus. The other two pseudocysts contained dark fluid and fragments of dead flukes. Although, no liver fluke eggs were detected in the faeces, thousands of eggs were found in the pseudocysts. Previous studies in Poland revealed two foci of this parasitosis: in south-western and south-eastern parts of the country. It is possible that the range of both foci is expanding due to red deer migrations and the spread of infected water snails and larval forms of the parasite in watercourses and rivers. Fascioloides magna infection is a threat for cervids and domestic ruminants. Therefore, it is important to monitor the occurrence of this fluke in Poland. It is essential to determine the range of fascioloidosis, examine the intermediate host species and record cases of F. magna infection in domestic ruminants..

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Section: Materiał I Metodyunclassified

Fascioloides magna: a parasite of roe deer in Bory Zielonogórskie

Demiaszkiewicz¹,

Kowalczyk²,

Filip³

et al. 2018

Medycyna Weterynaryjna

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“…PCR was performed on those samples as described previously. 6 Briefly, 20-50-mg sections of frozen liver from 1 fetal case and frozen liver or flukes from 10 adults with hepatic trematodosis in 2019-2021 were placed in a tube with 2 mL of 1 × PBS. Samples were homogenized (200 homogenizer; VWR) and centrifuged for 10 min at 2,780 × g. A total of 50 µL of the supernatant was transferred for extraction (MagMAX pathogen RNA/DNA kit; Applied Biosystems).…”

Section: Research-article2022mentioning

confidence: 99%

Gross and histologic description of trematodosis in fetal and neonatal beef calves in North Dakota and Minnesota

et al. 2022

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Hepatic trematodes, such as Fasciola hepatica and Fascioloides magna, have variable distribution throughout the United States. F. magna is endemic in the upper midwestern United States, and F. magna infections are diagnosed frequently in weaned calves and adult beef cattle at the North Dakota State University Veterinary Diagnostic Laboratory (NDSU-VDL). Rarely, liver fluke infestation has also been observed in much younger calves, including aborted fetuses. We describe here, in 2 fetal and 7 neonatal beef calves submitted to the NDSU-VDL between 2011 and 2020, parasitic migration tracts in livers, consisting of regionally extensive, random, linear tracts of fibrosis admixed with black porphyrin pigment, along with foci of necrosis and hemorrhage, and mixed inflammatory cells, which were caused presumptively by F. magna infection. Samples were not available from our 9 cases for PCR assay and sequencing, but we did confirm F. magna within liver samples collected from regional cattle in 2020 and 2021. Fetal and neonatal trematodosis was often concurrent with other common causes of fetal abortion and neonatal calf loss in our cases; however, based on the prepatent period of F. magna, fetal and neonatal beef calf trematode infestations occurred in utero.

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“…It was introduced to Europe together with the North American cervids, from where it spread to new species of definitive hosts: red deer, fallow deer and roe deer ( 10 , 15 ). Some natural fascioloidosis foci have become established in Europe, specifically in the Czech Republic, Italy, Croatia, Austria, Slovakia, Hungary and southwestern Poland ( 8 , 9 , 11 , 15 , 19 , 27 , 32 ).…”

Section: Introductionmentioning

confidence: 99%

“…Fascioloides magna demonstrates high pathogenicity for infected wildlife, and hence is of high veterinary importance ( 16 ). The migration of juvenile flukes and the fibrous encapsulation of adult trematodes inside the liver result in mechanical damage of the parenchyma, organ enlargement and possibly liver failure ( 8 ). Therefore, the presence of flukes is associated with significant losses in body girth and weight, as well as impaired reproduction in infected animals ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

The occurrence of Fascioloides magna (Bassi, 1875) in the wild cervid population in the Lower Silesian Wilderness – epidemiological and pathological aspects

Filip-Hutsch

Pyziel-Serafin

Hutsch³

et al. 2022

Journal of Veterinary Research

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Introduction Fascioloides magna is a parasite of high veterinary importance due to its pathogenicity for wild and domestic ruminants. The aim of our study was to describe the presence of trematode infection in the red deer population in the Lower Silesian Wilderness, one of the established fascioloidosis foci in Central Europe, and to assess the overall prevalence of F. magna in the studied area. In order to achieve this, a coprological study of different cervid species was performed. Material and Methods The livers of 99 red deer were collected over three years and examined for the presence of trematodes. Prevalence and infection intensity was estimated and a histopathological analysis was performed. In addition, 172 faecal samples from red deer, fallow deer and roe deer were examined. Results By year, Fascioloides magna was isolated from the livers of 2/30 (6.7%), 9/34 (26.5%) and 10/35 (28.6%) red deer. Severe hepatic lesions, including massive tissue damage, extensive fibrosis, and cirrhotic changes in the liver parenchyma were observed. Faecal examination revealed the presence of F. magna eggs, with a prevalence of approximately 40%, 50% and 53% in roe deer, fallow deer and red deer, respectively. Conclusion The eggs of F. magna may be commonly excreted in the faeces of roe deer, as well as those of red deer and fallow deer. The presence of F. magna throughout the cervid population in the Lower Silesian Wilderness favours the risk of the trematode’s transmission to livestock or farmed deer.

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Morphology and molecular study of Fascioloides magna – a growing threat to cervids (Cervidae) in Poland

Cited by 16 publications

References 15 publications

Fascioloides magna: a parasite of roe deer in Bory Zielonogórskie

Fascioloides magna: a parasite of roe deer in Bory Zielonogórskie

Gross and histologic description of trematodosis in fetal and neonatal beef calves in North Dakota and Minnesota

The occurrence of Fascioloides magna (Bassi, 1875) in the wild cervid population in the Lower Silesian Wilderness – epidemiological and pathological aspects

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