Morphology and Molecular Phylogeny of Two Marine Folliculinid Ciliates Found in China (Ciliophora, Heterotrichea)

Ye, Tingting; Zhao, Xuetong; Chi, Yong; Zheng, Bohan; Zhang, Hui; Huang, Jie; Warren, Alan; Chen, Xiangrui

doi:10.1007/s11802-021-4965-z

Cited by 7 publications

(2 citation statements)

References 43 publications

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“…Genomic DNA was extracted from the single cleaned cell using a DNeasy Blood & Tissue Kit (QIAGEN, Hilden, Germany) following the manufacturer's instructions but modified by using 1/4 of the suggested volume for each solution (Chi et al 2022a). SSU rDNA was amplified using Q5 Hot Start high-fidelity DNA polymerase (NEB, Ipswich, MA, USA) with the universal eukaryotic primers, 18 s-F (5'-AAC CTG GTT GAT CCT GCC AGT-3') and 18 s-R (5'-TGA TCC TTC TGC AGG TTC ACC TAC -3') (Medlin et al 1988;Ye et al 2021b). PCR amplifications were according to the following procedure: 1 cycle of initial denaturation at 98 ℃ for 30 s, followed by 18 cycles of amplification (98 ℃, 10 s; 69-51 ℃ touchdown, 30 s; 72 ℃, 1 min), and another 18 cycles (98 ℃, 10 s; 51 ℃, 30 s; 72 ℃, 1 min), with a final extension of 72 ℃ for 5 min (Ma et al 2022b;Zhang et al 2022b).…”

Section: Dna Extraction and Gene Sequencingmentioning

confidence: 99%

Exploring the biogeography, morphology, and phylogeny of the condylostomatid ciliates (Alveolata, Ciliophora, Heterotrichea), with establishment of four new Condylostoma species and a revision including redescriptions of five species found in China

Chi,

Wei,

Tang

et al. 2024

Mar Life Sci Technol

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Species of the ciliate class Heterotrichea Stein, 1859 are a cosmopolitan group of unicellular eukaryotic microorganisms, many of which have been widely used as models in various fields of research such as regenerative biology, functional ecology, environmental toxicology, and symbiotic behavior. However, species identification in the heterotrich family Condylostomatidae, especially the most species-rich and type genus Condylostoma Bory de Saint-Vincent, 1824, remains challenging due to incomplete original descriptions, few reliable distinguishing characters, and overlapping features between different species. This study presents an updated revision of Condylostoma and its related genus Condylostomides da Silva Neto, 1994 based on descriptions of five species, including nine populations collected from China, using both morphological and molecular methods. The main findings are as follows: (1) 43 nominal species and about 130 populations are reviewed, resulting in the recognition of 30 valid species of Condylostoma and eight valid species of Condylostomides; (2) keys, synonyms, biogeographic distributions and amended/improved diagnoses of all valid species are provided; (3) based on the available data, four new Condylostoma species (C. marinum sp. nov., C. petzi sp. nov., C. villeneuvei sp. nov., and C. microstomum sp. nov.), one new combination (Condylostomides minimus (Dragesco, 1954) comb. nov. & nom. corr.), and two corrected names (Condylostoma ancestrale Villeneuve-Brachon, 1940 nom. corr. and Condylostomides nigrus (Dragesco, 1960) nom. corr.) are suggested; (4) cryptic species are detected and proposed for the first time to form the Condylostoma curvum species complex; (5) three highly confusing Condylostoma species, C. kris, C. spatiosum, and C. minutum, are redefined for the first time based on modern taxonomic methods; (6) a ‘flagship’ species, Condylostomides coeruleus, is recorded for the first time from the continent of Asia, substantially expanding its biogeography; (7) ciliature adjacent to the distal end of the paroral membrane within the family Condylostomatidae is uniformly defined as frontal membranelles and is classified into three patterns according to the arrangement of kinetosomes, which serve as important key features.

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Section: Dna Extraction and Gene Sequencingmentioning

confidence: 99%

Exploring the biogeography, morphology, and phylogeny of the condylostomatid ciliates (Alveolata, Ciliophora, Heterotrichea), with establishment of four new Condylostoma species and a revision including redescriptions of five species found in China

Chi,

Wei,

Tang

et al. 2024

Mar Life Sci Technol

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“…To remedy eutrophication by nitrogen and phosphorus in nearshore aquaculture water, scholars have actively explored methods [9,10] such as algal-bacterial symbiosis [11], higher aquatic plants [12][13][14], microalgae [15], and physicochemical methods [16,17]. Among them, the use of seaweed cultivation for controlling eutrophication has been recently proved practical [18,19].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Role of Terminal Stolon of Marine Invasive Green Macroalga Caulerpa taxifolia in the Removal of Inorganic Nitrogen from Seawater

Bingxin

Wang

Chu

et al. 2022

JMSE

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The marine benthic green macroalga Caulerpa taxifolia is an invasive seaweed found in Europe, America, and Australia, and it forms into huge algal meadows on shallow seafloors with its stolon and rhizoid systems. It has bloomed along the coast of the South China Sea, causing serious environmental problems. However, its ecological impact has not been well studied. Therefore, this study investigated the changes in concentration of NH4-N and NO2-N in artificial seawater in which C. taxifolia was cultivated under laboratory conditions during the circadian rhythm. Results showed that concentrations of NH4-N and NO2-N decreased with the increase in culture time during the circadian rhythm. In 24 h, the NH4-N-removal efficiency increased during the light period, and the maximum reached 71.4%; that of NO2-N increased with time extension in the dark period, and the maximum reached 9.2%. The absorption of NH4-N and NO2-N by terminal stolon of C. taxifolia was different. NH4-N was absorbed more preferentially than NO2-N. However, there was no obvious correlation between NH4-N and NO2-N absorption. Therefore, the terminal stolon of C. taxifolia can be used to clean up inorganic nitrogen, and showed great application potential in the remediation of eutrophic waters as the algal-bacterial symbiotic system could facilitate NO2-N removal.

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From oral structure to molecular evidence: new insights into the evolutionary phylogeny of the ciliate order Sessilida (Protista, Ciliophora), with the establishment of two new families and new contributions to the poorly studied family Vaginicolidae

Warren

et al. 2023

Sci. China Life Sci.

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Morphology and Molecular Phylogeny of Two Marine Folliculinid Ciliates Found in China (Ciliophora, Heterotrichea)

Cited by 7 publications

References 43 publications

Exploring the biogeography, morphology, and phylogeny of the condylostomatid ciliates (Alveolata, Ciliophora, Heterotrichea), with establishment of four new Condylostoma species and a revision including redescriptions of five species found in China

Exploring the biogeography, morphology, and phylogeny of the condylostomatid ciliates (Alveolata, Ciliophora, Heterotrichea), with establishment of four new Condylostoma species and a revision including redescriptions of five species found in China

Investigating the Role of Terminal Stolon of Marine Invasive Green Macroalga Caulerpa taxifolia in the Removal of Inorganic Nitrogen from Seawater

From oral structure to molecular evidence: new insights into the evolutionary phylogeny of the ciliate order Sessilida (Protista, Ciliophora), with the establishment of two new families and new contributions to the poorly studied family Vaginicolidae

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