Morphology and Molecular Phylogeny of Two New Trachelophyllid Ciliates, Monolamellophrya terricola gen. nov., sp. nov. and Trachelophyllum parapiculatum sp. nov. (Litostomatea, Haptoria), From South Korea

Abstract

The morphology and molecular phylogeny of two new ciliates, Monolamellophrya terricola gen. nov., sp. nov. and Trachelophyllum parapiculatum sp. nov., discovered in South Korea, were investigated. The two species belong to the suborder Trachelophyllina, which is characterized by the presence of a mucilaginous layer containing lepidosomes covering the cortex. Monolamellophrya terricola gen. nov., sp. nov. is characterized by the presence of a single layer of type II lepidosomes, representing a new genus. Trachelophyllum parapiculatum sp. nov. has only type I lepidosomes covering the cortex, a generic character of the genus Trachelophyllum, and is distinguished from other congeners by a combination of morphological features, including the 15–24 μm long rod-shaped extrusomes, the 9–13 ciliary rows, the 7–11 and 17–25 dikinetids in brush rows 1 and 2, respectively, and the bipolar brush row 3. Furthermore, the 18S rRNA gene sequences of the two new species were provided. The phylogenetic analyses show that the sequence of M. terricola gen. nov., sp. nov. clusters with two other trachelophyllid sequences, and the sequence of T. parapiculatum sp. nov. is placed at the base of these three sequences with full support. Furthermore, the four trachelophyllid sequences that are available so far form a monophyletic clade.

Highly divergent morphology but a close molecular phylogenetic relationship between two little‐known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species

“…Spathidiida include at least 12 families and over 250 species, about half of which still lack modern taxonomic data (Foissner, 2016; Foissner & Xu, 2007; Vďačný et al., 2011). Recent reports indicate a substantial unexplored biodiversity within this group (Barmshuri et al., 2023; Bourland, 2023; Chi, Liu, et al., 2022; Foissner & Berger, 2021; Omar et al., 2022; Wang et al., 2022). Furthermore, the phylogenetic relationships among spathidiids remain unresolved, primarily due to short internodes and weak statistical support for deeper nodes in 18S rRNA gene trees.…”