Morphology and microfilament organization in human blood lymphocytes *1Effects of substratum and mitogen exposure

Sundqvist, Kristina; Otteskog, Per; Wanger, L; Thorstensson, Rigmor; Utter, Göran

doi:10.1016/0014-4827(80)90009-9

Cited by 13 publications

(4 citation statements)

References 19 publications

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“…Villous projections of attached lymphocytes (Anderson and Ander-son, 1976) and of lymphocytes during early stages of endothelial penetration (Cho and DeBruyn, 1979) have been reported previously. An interesting correlate of these observations is that villous projections of lymphocytes have been shown to contain high concentrations of actin (Sundqvist et al, 1980), a substance involved in adhesion and locomotion of several cell types, including lymphocytes (reviewed by Parrott and Wilkinson, 1981). If the intercellular contacts are adherent sites for locomotion of the lymphocytes, one would expect concentration of actin near the contacts.…”

Section: Discussionmentioning

confidence: 95%

Intercellular contacts of lymphocytes during migration across high‐endothelial venules of lymph nodes. An electron microscopic study

Campbell

1983

Anat. Rec.

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The migration of lymphocytes across the wall of high-endothelial venules was studied by electron microscopic examination of murine lymph nodes fixed with glutaraldehyde and tannic acid. Regions of close membrane apposition, referred to in the present study as "intercellular contacts," were observed between migrating lymphocytes and endothelial cells of the vessel wall. At high magnification the intercellular contacts resolve into pentalaminar structures resembling gap junctions. However, the location of these contacts suggests that they are regions of membrane adherence utilized for locomotion of the lymphocytes across the endothelium. At present, it is unclear whether these intercellular contacts are communicating junctions or sites of membrane adherence.

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Section: Discussionmentioning

confidence: 95%

Intercellular contacts of lymphocytes during migration across high‐endothelial venules of lymph nodes. An electron microscopic study

Campbell

1983

Anat. Rec.

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“…The main components of the cytoskeleton (microfilaments, microtubules, and intermediate filaments) occur in the cytoplasm of lymphocytes (Yahara and Edelman, 1975). Dense microfilamentous networks are observed underneath the plasma membrane (Yahara and Edelman, 1975;Sundqvist et al, 1980;Fagraeus et al, 1974;de Petris, 1975) as well as in regions close to the nuclear membrane (Yahara and Edelman, 1975). The microtubules are usually separated from the plasma membrane by microfilaments (de Petris, 1975).…”

Section: Discussionmentioning

confidence: 99%

Effect of aniosmotic media on the volume of the T-lymphocyte nucleus

Hoekstra

Aten

Sloot

1991

Biophysical Journal

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Time-resolved measurements of the nuclear volume response of human peripheral T-lymphocytes, under aniosmotic conditions, are presented. In the experiments slit scanning FlowCytometry methods were used. We propose an extension to the standard solid viscoelastic model to interpret the observed dynamical behavior of the nucleus. It is shown that experimental and theoretical evidence indicates a passive nuclear response merely induced by a mechanical link (i.e., the cytoskeleton) between the cell membrane and the nuclear envelope. Implications of this work to the field of cellular mechanics and cytoskeletal rheology are surveyed.

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“…When choosing an immobilization strategy, the user should keep in mind that this might affect the native state, structure, or functionality of the cell. For example, concanavalin A is known to elicit changes in the functional state of mononuclear phagocytes and lymphocytes, , and the mechanical trapping of living cells can affect their mechanical properties . Immobilization may be also facilitated by underpressure (suction), by, for example, using cantilevers with integrated microchannels, , although cells can change state and adjust properties in response to pressure changes …”

Section: Principles and Limitations Of (Bio-)chemical Afm Imagingmentioning

confidence: 99%

Atomic Force Microscopy-Based Force Spectroscopy and Multiparametric Imaging of Biomolecular and Cellular Systems

et al. 2020

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During the last three decades, a series of key technological improvements turned atomic force microscopy (AFM) into a nanoscopic laboratory to directly observe and chemically characterize molecular and cell biological systems under physiological conditions. Here, we review key technological improvements that have established AFM as an analytical tool to observe and quantify native biological systems from the micro-to the nanoscale. Native biological systems include living tissues, cells, and cellular components such as single or complexed proteins, nucleic acids, lipids, or sugars. We showcase the procedures to customize nanoscopic chemical laboratories by functionalizing AFM tips and outline the advantages and limitations in applying different AFM modes to chemically image, sense, and manipulate biosystems at (sub)nanometer spatial and millisecond temporal resolution. We further discuss theoretical approaches to extract the kinetic and thermodynamic parameters of specific biomolecular interactions detected by AFM for single bonds and extend the discussion to multiple bonds. Finally, we highlight the potential of combining AFM with optical microscopy and spectroscopy to address the full complexity of biological systems and to tackle fundamental challenges in life sciences.

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Morphology and microfilament organization in human blood lymphocytes *1Effects of substratum and mitogen exposure

Cited by 13 publications

References 19 publications

Intercellular contacts of lymphocytes during migration across high‐endothelial venules of lymph nodes. An electron microscopic study

Intercellular contacts of lymphocytes during migration across high‐endothelial venules of lymph nodes. An electron microscopic study

Effect of aniosmotic media on the volume of the T-lymphocyte nucleus

Atomic Force Microscopy-Based Force Spectroscopy and Multiparametric Imaging of Biomolecular and Cellular Systems

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