Morphology and Matrix Composition During Early Tendon to Bone Healing

Liu, Stephen H.; Panossian, Vah; Al‐Shaikh, Raad; Tomin, Emre; Shepherd, Eric F.; Finerman, Gerald A. M.; Lane, Joseph M.

doi:10.1097/00003086-199706000-00034

Cited by 181 publications

(129 citation statements)

References 16 publications

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“…The bone-tendon junction is regenerated after surgical reconstruction of an enthesis [2,6,9,13,14,18,22,24,25,311. Formation of collagen fibers anchoring into bone has generally been regarded as a critical event in recovery [9,31].…”

Section: Introductionmentioning

confidence: 99%

Early anchoring collagen fibers at the bone—tendon interface are conducted by woven bone formation: light microscope and scanning electron microscope observation using a canine model

Oguma

Murakami

Takahashi-Iwanaga

et al. 2001

Journal Orthopaedic Research

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To clarify the early process of recovery at the bone-tendon interface, we used light microscopy and SEM to examine the process of anchoring of collagen fibers to bone in a canine model.At two weeks, tendon, scar tissue, woven bone and lamellar bone were present at the insertion site. SEM revealed anchoring of collagen fibril bundles of the scar to the woven bone. By 4 weeks, the number of anchoring fibers had increased and a parallel arrangement of fibers was observed. SEM demonstrated deep penetration of fibers into the woven bone layer. In addition, the fibers were observed to project into and intermingle with the scar tissue. By 6 weeks, the anchoring fibers had developed fully and were distributed densely over the interface. SEM also revealed that the collagen fibril bundles in the scar tissue had connected with the collagen fibrils of the woven bone by way of the anchoring bundles. The woven bone was identifiable throughout the early stages of recovery as the interface between soft tissue and hard tissue. Throughout all experimental periods, no staining was observed at the interface of the tendon and bone by Saffranin-0. The formation of woven bone was important during early recovery of the tendonbone interface prior to the completion of fibrocartilage-mediated insertion. 0

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Section: Introductionmentioning

confidence: 99%

Early anchoring collagen fibers at the bone—tendon interface are conducted by woven bone formation: light microscope and scanning electron microscope observation using a canine model

Oguma

Murakami

Takahashi-Iwanaga

et al. 2001

Journal Orthopaedic Research

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“…(3) The maximum pull-out load of the tendon measured in this study was smaller than that in the studies of tendon reattachment to bone [2,8,9,10,13], and even smaller than that in our previous study [7]. In previous reports evaluating tendon healing in a bone tunnel, a fibrocartilagenous layer and/or collagen fibres penetrating the tendon from the bone were observed at the interface [2,5,8,9,10,13]. In this study, abundant bone formation around the tendon was observed in the BMSC(+) group 6 weeks after the operation, and collagen continuity between the tendon and fibrous tissue in the pores and direct apposition of tendon to bone were observed in some pores; however, a fibrocartilagenous layer and penetrating collagen fibres were not observed in the interface.…”

Section: Discussionmentioning

confidence: 52%

Augmentation of tendon attachment to porous ceramics by bone marrow stromal cells in a rabbit model

Omae

Mochizuki

Yokoya

et al. 2006

International Orthopaedics (SICOT)

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Tendon attachment to interconnected porous calcium hydroxyapatite ceramics (IP-CHA) with cultured bone marrow stromal cells (BMSC) was analysed. The purpose of this study was to evaluate whether BMSC in IP-CHA could augment the tendon attachment to IP-CHA histologically and biomechanically. Eighteen Japanese white rabbits were used. Cultured BMSCs were subcultured in IP-CHA. The grafted tendon and IP-CHA with BMSC complex were implanted in a bone defect of the knee [BMSC(+) group]. In the contralateral knee, a tendon and IP-CHA without BMSC complex were implanted [BMSC(-) group]. Histological findings of the interface between the tendon and IP-CHA were similar in the two groups 3 weeks after the operation. However, 6 weeks after the operation, more abundant bone formation around the tendon was observed in the BMSC (+) group. The direct apposition of the tendon to bone in pores and collagen fibre continuity between the tendon and fibrous tissue in pores were observed. In biomechanical evaluation, the maximum pull-out load of the tendon from the IP-CHA in the BMSC(+) group was significantly higher than that in the BMSC(-) group 6 weeks after the operation. BMSCs cultured in IP-CHA could augment tendon attachment to IP-CHA. Résumé Le propos de cette étude est de déterminer si l'utilisation de culture cellulaire de moelle osseuse (BMSC) avec l'utilisation de calcium d'hydroxyapatite (IP-CHA) augmente la résistance de l'attachement osseux du tendon sur le plan histologique et biomécanique. Dix-huit lapins blancs du Japon ont été utilisés pour ce travail. Les tendons greffés avec hydroxyapatite et culture de la cellule de moelle osseuse ont été implantés dans une defect au niveau du genou. Sur le genou opposé, le tendon a été implanté sans culture cellulaire. Sur le plan histologique les résultats sont identiques dans les deux groupes, 3 semaines après intervention, cependant 6 semaines après l'intervention la progression de la formation osseuse est plus importante autour du tendon ayant bénéficié d'une culture cellulaire de moelle osseuse. Il en est de même en ce qui concerne la résistance biomécanique du tendon qui est significativement plus importante dans le groupe culture cellulaire, 6 semaines après l'intervention.

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“…There are no analogous sites in humans where a native tendon passes through a bone tunnel, however, and the biology of this unique environment remains incompletely understood. Wide variations in the morphological characteristics of the tendon-bone attachment site have been reported [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]. Some studies have demonstrated formation of a native, direct type of insertion and an intermediate fibrocartilage zone at the interface.…”

Section: Discussionmentioning

confidence: 99%

Differences in Tendon Graft Healing between the Intra-articular and Extra-articular Ends of a Bone Tunnel

et al. 2009

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The basic biology of healing between a tendon graft and bone tunnel remains incompletely understood. Distinct variability in the morphological characteristics of the healing tendon-bone attachment site has been reported. We hypothesized that spatial and temporal differences in tendon-to-bone healing exist at different regions of a surgically created bone tunnel. Twenty-four male, Sprague-Dawley rats underwent anterior cruciate ligament (ACL) reconstruction in the left knee using a flexor digitorum longus tendon graft secured using suspensory periosteal fixation.

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Morphology and Matrix Composition During Early Tendon to Bone Healing

Cited by 181 publications

References 16 publications

Early anchoring collagen fibers at the bone—tendon interface are conducted by woven bone formation: light microscope and scanning electron microscope observation using a canine model

Early anchoring collagen fibers at the bone—tendon interface are conducted by woven bone formation: light microscope and scanning electron microscope observation using a canine model

Augmentation of tendon attachment to porous ceramics by bone marrow stromal cells in a rabbit model

Differences in Tendon Graft Healing between the Intra-articular and Extra-articular Ends of a Bone Tunnel

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