Morphology and Lectin histochemistry of the testes of brown-banded bamboo shark (Chiloscyllium punctatum)

Abstract

Lectin histochemistry of the testes of shark Kassab et al.

Histological and Morphological Aspects of Reproduction in Male Blackspotted Smooth-HoundMustelus punctulatusin the Adriatic Sea (Eastern Mediterranean Sea)

“…Microscopic observation of M. punctulatus testes revealed a diametric development, in which round-shaped spermatocysts have zonal arrangement. Diametric development, in which spermatogenesis extends from one wall across the diameter of the testis to the opposite wall with efferent ducts [16], was described for the shark families Carcharhinidae, Sphyrnidae, Somniosidae, Centrophoridae, Hemiscylliidae, and Triakidae [17][18][19][20]. The gross testis morphology, association of testis with the epigonal organ, and microscopic anatomy described in this study correspond with the previous studies on closely related sharks [17].…”

Comparison of the Morphology and Histomorphometry of Spermatogenic Cyst of Three Sharks Species With Diametric Testes

“…Diametric testicular development is characteristic of Carcharhinidae and Sphyrnidae testes. In the last 10 years, testes of Triakidae (Rogers, 2013), Somniosidae, Centrophoridae (Girard et al, ) and Hemiscyliidae (Kassab et al, ) shark families have also been recognized to exhibit diametric type of development. In the present study P. glauca and R. lalandii are members of Carcharhinidae family and M. canis is member of Triakidae family.…”

The Distribution Profile of Glycoconjugates in the Testis of Brown-Banded Bamboo Shark (Chiloscyllium punctatum) by Using Lectin Histochemistry

“…The present work revealed that the germ cells of the spermatocyte zone expressed all sugars under investigation except fucose (UEA-1), while Kassab et al (2009) reported that the spermatocytes only reacted to the galactose and glucosamine. On the other hand, GlcNAc is prevalent in all the germ cell types in the frog ( Xenopus laevis ) testis and can be identified in the spermatogonial cells (Valbuena et al, 2012).…”

“…The current investigation showed that the neutral mucopolysaccharides, identified by the PAS labeling, appeared in the interstitial tissue of the spermatogonial, spermatocyte, and spermatid zones and became more apparent in the spermatozoal–Sertoli cell junction in the spermatozoal zone. These data suggest a role of neutral mucopolysaccharides during the development of shark spermatozoa as well as Sertoli–spermatozoal attachment (Kassab et al, 2009). Also, it was reported that there was a progressive increase in glycosylation during spermatogenesis, especially at the level of the acrosome in the spermatocyte-spermatid step (Liguoro et al, 2004).…”

“…Despite the many studies on shark (Matthews, 1950; Stanley, 1966; Dobson & Dodd, 1977; Pudney & Callard, 1984 a , 1984 b ; Pratt, 1988; Parsons & Grier, 1992; Pratt & Tanaka, 1994; Girard et al, 2000; Liguoro et al, 2004), few studies on the testis of bamboo shark (Kassab et al, 2009; Farrag et al, 2018) have been restricted to their general morphology; only peroxidase histochemistry was used to clarify the glycan distribution. This study aimed to investigate, via immunofluorescence histochemistry, the staining pattern of a panel of 12 lectins in the germ, somatic, and interstitial cells to analyze the cellular distribution of glycoconjugates, hoping to shed light on the possible functions of lectins in sperm development, maturation, and release from the spermatocyst.…”