Morphology and growth potential of stromal cell cultures derived from human endometrium

Abstract

Propagable cell cultures derived from human endometrial tissue were determined to contain cells predominantly of stromal cell origin based on their morphologic resemblance to endometrial stromal cells. These features included nexi, solitary cilia, and predecidual cytology. In addition to morphology the cell cultures retained a normal karyotype and responded to steroid hormones as evidenced by cellular aggregation. The stromal cells were evaluated for a variety of characteristics associated with transformed cells and seemed to be biologically normal without neoplastic phenotypes. Growth potential of the stromal cell cultures was also characterized in normal maintenance medium, in nutritionally depleted medium with reduced levels of calcium or serum, and in medium with increased levels of serum. The prolonged survival of the stromal cells in vitro coupled with the retention of in vivo characteristics and an absence of neoplastic phenotype provides a human cell system that is amenable to a variety of long-term experimental analyses.

Association between Endometriosis and Heavy Metals in a Group of Sri Lankan Women of Reproductive Age

“…The morphology of individual stromal cells observed in the present study is in agreement with previous reports where isolated stromal cells are described as elongated fibroblast like cells with central nuclei (Satyaswaroop, Bressler, La-Pena and Gurpide, 1979;Dorman, Varma, Siegfried, Melin et al, 1982). In this study, the purity of the isolated stromal cells was confirmed by immunohistochemical staining using primary antibodies for vimentin and cytokeretin where more than 95% of the stromal cells gave positive staining for vimentin and negative for cytokeratin.…”

“…Different nuirient culture media such as DMEMI P12 (Matthews, Redfern, Hirst and Thomas, 1992), MEM a (Satyaswaroop, Bressler, La-Pena and Gurpide, 1979), CMRL-1066 (Dorman, Varma, Siegfried, Melin et al, 1982) and RPMI-1640 (Overton, Fernàndez-Shaw, I-licks, Barlow ci at,, 1997;Finas, Huszar, Agic, Dogan et aL, 2008), have been used by researchers in the maintenance of endometrial stromal cell cultures.…”

“…During the passages and long term cultures, the stromal cells retained their immunogenicity to vimentin as described in section 4. (Dorman, Varma, Siegfried, Melin et al, 1982;Holinka and Gurpide, 1987).…”

Enhancement by TPA of phenotypes associated with transformation in carcinogen‐treated human cells: Evidence for a selective mechanism

“…Human endometrial stromal cell cultures were established from hysterectomy specimens as described previously (Varma et al, 1982;Dorman et al, 1982). Cells were established as primary cultures and were subcultured three times before carcinogen exposure.…”

Malignant Transformation of Human Endometrial Stromal Cells by Transfection of <i>c-my</i><i>c</i>: Effects of pRSV<i>neo</i> Cotransfection and Treatment with MNNG

“…Methods to establish cultures of normal human endometrial stromal cells from hysterectomy specimens have been described pre viously [17). Primary and monolayer cultures of stromal cells were maintained in RPMI 1640 medium (Gibco Laboratories, Grand Island, N.Y., USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan, Utah, USA) and 2 pg/ml of insulin (Sigma Chemical Co., St. Louis, Mo., USA).…”