Morphology and function of rooster efferent ductule epithelial cells in culture

Janssen, Sarah; Bunick, David; Finnigan-Bunick, Carrol; Chen, Y.C.; Hess, Rex A.; Bahr, Janice M.

doi:10.1016/s0040-8166(98)80036-0

Cited by 10 publications

(4 citation statements)

References 28 publications

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“…Immunohistochemical localization of p450 aromatase (CYP19A1) in the rat epididymis. See prior study (Janssen et al, 1998) for immunohistochemical method and control. (A) Caput region showing intense staining of the cytoplasmic droplet of luminal sperm.…”

Section: Estrogen Concentrations In Rete Testis Fluid and Semenmentioning

confidence: 99%

Estrogen and Its Receptors in Efferent Ductules and Epididymis

Hess

Fernandes

Gomes

et al. 2011

Journal of Andrology

112

102

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Estrogens play key roles in the development and maintenance of male reproductive function and fertility. In this review, we briefly describe the localization and function of estrogen receptors ESR1 and ESR2 (also known as ERa and ERb, respectively) and the expression of G protein-coupled estrogen receptor-1 (GPER, formerly known as GPR30) in efferent ductules and epididymis. The efferent ductules present the highest levels of ESR1 and ESR2 in the male reproductive system, and represent a major target of estrogen action. In efferent ductules, ESR1 has a crucial role in the regulation of fluid reabsorption, and in the epididymis the receptor helps to maintain fluid osmolality and pH. ESR1 expression in the epididymal epithelium shows considerable variation among species, but differences in laboratory techniques may also contribute to this variation. Here we report that Esr1 mRNA and protein are higher in corpus than in other regions of the rat epididymis. The mRNA level for Gper was also higher in corpus. Although ESR1 is expressed constitutively in efferent ductules and down-regulated by estrogen, in the epididymis, both testosterone (T) and estradiol (E2) may regulate its expression. T and E2 are, respectively, higher and lower in the corpus than in the initial segment/caput and cauda regions. It is important to determine the expression of GPER, ESR1, androgen receptor, and their respective cofactors in specific cell types of this tissue, as well as the intracellular signaling pathways involved in efferent ductules and epididymis. These studies will help to explain the consequences of exposures to environmental endocrine disruptors and provide potential targets for the development of a male contraceptive.

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Section: Estrogen Concentrations In Rete Testis Fluid and Semenmentioning

confidence: 99%

Estrogen and Its Receptors in Efferent Ductules and Epididymis

Hess

Fernandes

Gomes

et al. 2011

Journal of Andrology

112

102

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“…It is believed that in birds the growth of the male genital tract depends on androgen (Lofts & Massa 1980). Janssen et al (1998) found that a physiological level of hydroxytestosterone was required for culture of the epithelial cells of the epididymis. Therefore, the interactions of androgen and the AR in the epididymis and ductus deferens may play an essential role in the growth of these tissues.…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of sex steroid receptors in the epididymis and ductus deferens of immature and mature Japanese Quail, Coturnix Japonica

Nishizawa

Okamoto

Yoshimura

2002

Animal Science Journal

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The goal of this study was to determine whether in the Japanese quail the male genital tract contains receptors for progesterone, androgen and estrogen (PR, AR and ER, respectively), which have significant roles in reproductive functions, and whether their localization changes during sexual maturation. The epididymis and ductus deferens (middle and ampulla regions) of immature (approximately 30-day-old) and mature male Japanese quail were collected and frozen sections of them were immunostained for PR, AR and ER. The immunoreaction products for AR and PR were found in the nuclei of epithelial cells in the efferent ductules, epididymal duct, and the middle and ampulla regions of the ductus deferens of mature and immature birds. In the mature birds, the epithelial cells of the efferent ductules, epididymal duct, and the middle and ampulla regions of the ductus deferens were positive for ER, although some of the cells in the ductus deferens were negative. The epithelial cells of the ductules in the epididymis stained positive for ER, but the immunoreactions were negligible in the ductus deferens of immature birds. These results suggest that the epididymis and ductus deferens in quail possesses PR, AR and ER receptors. Each receptor is expressed before sexual maturation, although enhancement of ER expression may occur during maturation.

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“…Although sperm traverse this region of the male reproductive tract rapidly, these ducts resorb nearly 90% of the testicular plasma output before the sperm are stored for an extended period in the ductus deferens [8]. The epididymal region of the male reproductive tract is therefore essential for sperm maturation, and dysfunction of this region results in infertility [9, 10, 11]. …”

Section: Introductionmentioning

confidence: 99%

Localization of S-100 proteins in the testis and epididymis of poultry and rabbits

2014

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The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal duct as well as the principal cells of the rabbit epididymis. In conclusion, the distribution of the S-100 proteins in the testis and epididymis might point out to its roles in the male reproduction.

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Morphology and function of rooster efferent ductule epithelial cells in culture

Cited by 10 publications

References 28 publications

Estrogen and Its Receptors in Efferent Ductules and Epididymis

Estrogen and Its Receptors in Efferent Ductules and Epididymis

Immunolocalization of sex steroid receptors in the epididymis and ductus deferens of immature and mature Japanese Quail, Coturnix Japonica

Localization of S-100 proteins in the testis and epididymis of poultry and rabbits

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