Morphology and electrostatics play active role in neuronal differentiation processes on flexible conducting substrates

Srivastava, Nishit; James, Jackson; Narayan, K. S.

doi:10.4161/org.27213

Cited by 8 publications

(11 citation statements)

References 45 publications

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“…To investigate the role of type IV collagen in septation, we used an epithelial–mesenchymal co-culture model of alveolar morphogenesis [ 50 ]. This model produces peaks of mesenchymal cells covered by an epithelial lining.…”

Section: Resultsmentioning

confidence: 99%

“…This model produces peaks of mesenchymal cells covered by an epithelial lining. The central core of mesenchymal cells closely resembles the myofibroblasts seen during alveolar septal development in vivo [ 50 ]. When MRC-5 fetal mouse mesenchymal cells were co-cultured with human A549 epithelial cells, septal-like formations appeared after 4–5 days and reached their maximum after 7 days (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To address a direct effect of type IV collagen in septal formation we used an in vitro co-culture of mesenchymal–epithelial cells designed to resemble alveolar septal formation [ 50 ]. A549 epithelial cells share properties with immature alveolar type II pneumocytes but not of type I pneumocytes (data not shown and [ 67 , 68 ]).…”

Section: Discussionmentioning

confidence: 99%

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Type IV collagen drives alveolar epithelial–endothelial association and the morphogenetic movements of septation

et al. 2016

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BackgroundType IV collagen is the main component of the basement membrane that gives strength to the blood–gas barrier (BGB). In mammals, the formation of a mature BGB occurs primarily after birth during alveologenesis and requires the formation of septa from the walls of the saccule. In contrast, in avians, the formation of the BGB occurs rapidly and prior to hatching. Mutation in basement membrane components results in an abnormal alveolar phenotype; however, the specific role of type IV collagen in regulating alveologenesis remains unknown.ResultsWe have performed a microarray expression analysis in late chick lung development and found that COL4A1 and COL4A2 were among the most significantly upregulated genes during the formation of the avian BGB. Using mouse models, we discovered that mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. Mutations in Col4a1 derived from the vascular component were sufficient to cause defects in vascular development and the BGB. We also show that Col4a1 and Col4a2 mutants displayed disrupted myofibroblast proliferation, differentiation and migration. Lastly, we revealed that addition of type IV collagen protein induced myofibroblast proliferation and migration in monolayer culture and increased the formation of mesenchymal–epithelial septal-like structures in co-culture.ConclusionsOur study showed that type IV collagen and, therefore the basement membrane, play fundamental roles in coordinating alveolar morphogenesis. In addition to its role in the formation of epithelium and vasculature, type IV collagen appears to be key for alveolar myofibroblast development by inducing their proliferation, differentiation and migration throughout the developing septum.Electronic supplementary materialThe online version of this article (doi:10.1186/s12915-016-0281-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Type IV collagen drives alveolar epithelial–endothelial association and the morphogenetic movements of septation

et al. 2016

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“…Several studies have shown that the transplantation of cardiomyocyte patches can improve cardiac function after a myocardial infarction. 20,22,36 Rather than serving a therapeutic role, the microvessel patches in the present study could be combined with a cardiomyocyte patch to provide rapid blood flow to the implanted heart patch. This could allow for the implantation of larger patches and higher numbers of cardiomyocytes.…”

Section: Discussionmentioning

confidence: 99%

Inosculation and perfusion of pre-vascularized tissue patches containing aligned human microvessels after myocardial infarction

et al. 2016

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A major goal of tissue engineering is the creation of pre-vascularized tissues that have a high density of organized microvessels that can be rapidly perfused following implantation. This is especially critical for highly metabolic tissues like myocardium, where a thick myocardial engineered tissue would require rapid perfusion within the first several days to survive transplantation. In the present work, tissue patches containing human microvessels that were either randomly oriented or aligned were placed acutely on rat hearts post-infarction and for each case it was determined whether rapid inosculation could occur and perfusion of the patch could be maintained for 6 days in an infarct environment. Patches containing self-assembled microvessels were formed by co-entrapment of human blood outgrowth endothelial cells and human pericytes in fibrin gel. Cell-induced gel contraction was mechanically-constrained resulting in samples with high densities of microvessels that were either randomly oriented (with 420±140 lumens/mm2) or uniaxially aligned (with 940±240 lumens/mm2) at the time of implantation. These patches were sutured onto the epicardial surface of the hearts of athymic rats following permanent ligation of the left anterior descending artery. In both aligned and randomly oriented microvessel patches, inosculation occurred and perfusion of the transplanted human microvessels was maintained, proving the in vivo vascularization potential of these engineered tissues. No difference was found in the number of human microvessels that were perfused in the randomly oriented (111±75 perfused lumens/mm2) and aligned (173±97 perfused lumens/mm2) patches. Our results demonstrate that tissue patches containing a high density of either aligned or randomly oriented human pre-formed microvessels achieve rapid perfusion in the myocardial infarct environment - a necessary first-step toward the creation of a thick, perfusable heart patch.

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“…In general, polymer surfaces with appropriate mechanical attributes such as adhesive strength, microtexture and nanotopography, surface wettability and stiffness are conducive for anchoring neuronal cells. 2,3 The electronic characteristics of certain conjugated polymers have also been used for sensing and stimulating neuronal activity. [4][5][6][7] The optoelectronic properties of these materials have recently been utilized as active triggers for neuronal stimulation.…”

Section: Please Scroll Down For Articlementioning

confidence: 99%

Polymer optoelectronic structures for retinal prosthesis

Gautam

Narayan

2014

Organogenesis

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This commentary highlights the effectiveness of optoelectronic properties of polymer semiconductors based on recent results emerging from our laboratory, where these materials are explored as artificial receptors for interfacing with the visual systems. Organic semiconductors based polymer layers in contact with physiological media exhibit interesting photophysical features, which mimic certain natural photoreceptors, including those in the retina. The availability of such optoelectronic materials opens up a gateway to utilize these structures as neuronal interfaces for stimulating retinal ganglion cells. In a recently reported work entitled "A polymer optoelectronic interface provides visual cues to a blind retina," we utilized a specific configuration of a polymer semiconductor device structure to elicit neuronal activity in a blind retina upon photoexcitation. The elicited neuronal signals were found to have several features that followed the optoelectronic response of the polymer film. More importantly, the polymer-induced retinal response resembled the natural response of the retina to photoexcitation. These observations open up a promising material alternative for artificial retina applications.

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Morphology and electrostatics play active role in neuronal differentiation processes on flexible conducting substrates

Cited by 8 publications

References 45 publications

Type IV collagen drives alveolar epithelial–endothelial association and the morphogenetic movements of septation

Type IV collagen drives alveolar epithelial–endothelial association and the morphogenetic movements of septation

Inosculation and perfusion of pre-vascularized tissue patches containing aligned human microvessels after myocardial infarction

Polymer optoelectronic structures for retinal prosthesis

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