Morphology and Distribution of Sensilla on the Antennae of Hylamorpha elegans Burmeister (Coleoptera: Scarabaeidae)

Mutis, Ana; Palma, Rubén; Parra, Leonardo; Alvear, Marysol; Isaacs, Rufus; Morón, Miguel-Ángel; Quiróz, Andrés

doi:10.1007/s13744-014-0208-y

Cited by 19 publications

(19 citation statements)

References 26 publications

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“…In most scarab beetles, as in the generality of insects, sex pheromones are released by the females to attract males, and therefore the number of pheromone‐sensitive sensilla (placodea, in case of scarab beetles) is much greater in males (Kim and Leal, ; Leal, ). A similar sexual dimorphism seems to be a rule in Rutelini (Ågren, ; Kim and Leal, ; Mutis et al, ) and Melolonthini (Allsopp, ; Romero‐López et al, ) (Table ), but is not observed in O. eremita . Different density and distribution of olfactory sensilla in various species may reflect different ways in the use of sexual pheromones for intraspecific communication.…”

Section: Discussionmentioning

confidence: 83%

Antennal fine morphology of the threatened beetle Osmoderma eremita (Coleoptera: Scarabaeidae), revealed by scanning electron microscopy

Zauli

Maurizi

Carpaneto

et al. 2016

Microscopy Res & Technique

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The aim of this study was to characterize the antennal morphology of Osmoderma eremita, a threatened scarab beetle inhabiting tree hollows. O. eremita males produce a sex pheromone, (R)-(+)-γ-decalactone, responsible mainly for the attraction of females but also other males. Gross and fine morphology of microstructures including sensilla, microsculpture and pores were analyzed using Scanning Electron Microscopy. The antenna of O. eremita showed the typical lamellicorn shape of scarab beetles, with a basal scape, a pedicel, a funicle composed of five antennomeres and a club composed of three lamellae. Six different subtypes of sensilla chaetica (Ch.1 - 6), Böhm sensilla (Bo), one subtype of sensilla basiconica (Ba.1), two subtypes of sensilla coeloconica (Co.1 - 2), two subtypes of sensilla placodea (Pl.1 - 2), pores and peculiar folds were described. The two sexes did not show any significant differences in the occurrence and number of the sensilla placodea, known to be responsible for the pheromone reception. Instead, some sexual differences were found on the occurrence and topology of three different microstructures: (1) one subtype of sensillum chaeticum (Ch.2) occurring on the pedicel only in males; (2) a characteristic pore occurring on the funicle only in males; (3) a peculiar fold occurring on different antennomeres of the funicle in the two sexes, on the fourth in males and on the fifth in females. A comparison between sensilla of O. eremita and those of other Scarabaeoidea is provided.

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Section: Discussionmentioning

confidence: 83%

Antennal fine morphology of the threatened beetle Osmoderma eremita (Coleoptera: Scarabaeidae), revealed by scanning electron microscopy

Zauli

Maurizi

Carpaneto

et al. 2016

Microscopy Res & Technique

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“…Subsequently, a lateral lamella was excised in order to expose one side of the middle lamella, where olfactory sensilla are located (Mutis et al . ). Signals from antennae were conducted and recorded by Syntech equipment (Kirchzarten, Germany).…”

Section: Methodsmentioning

confidence: 97%

“…A recent study on morphology and distribution of sensilla suggests that males of H. elegans have more chemosensory sensilla than females because males are able to find and recognize females while they are feeding on leaves of N. obliqua (Mutis et al . ). Another special behavior has been highlighted for this beetle: after copulation females fly to crops such as red clover, Trifolium pratense , to deposit their fertilized eggs (Artigas ).…”

Section: Introductionmentioning

confidence: 97%

β‐Ionone as putative semiochemical suggested by ligand binding on an odorant‐binding protein of Hylamorpha elegans and electroantennographic recordings

Venthur

Zhou

Mutis

et al. 2016

Entomological Science

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Currently, odorant‐binding proteins (OBPs) are considered the first filter for olfactory information for insects and constitute an interesting target for pest control. Thus, an OBP (HeleOBP) from the scarab beetle Hylamorpha elegans (Burmeister) was identified, and ligand‐binding assays based on fluorescence and in silico approaches were performed, followed by a simulated binding assay. Fluorescence binding assays showed slight binding for most of the ligands tested, including host‐plant volatiles. A high binding affinity was obtained for β‐ionone, a scarab beetle‐related compound. However, the binding of its analogue α‐ionone was weaker, although it is still considered good. On the other hand, through a three‐dimensional model of HeleOBP constructed by homology, molecular docking was carried out with 29 related ligands to the beetle. Results expressed as free binding energy and fit quality (FQ) indicated strong interactions of sesquiterpenes and terpenoids (α‐ and β‐ionone) with HeleOBP as well as some aromatic compounds. Residues such as His102, Tyr105 and Tyr113 seemed to participate in the interactions previously mentioned. Both in silico scores supported the experimental affinity for the strongest ligands. Therefore, the activity of α‐ionone, β‐ionone and 2‐phenyl acetaldehyde at antennal level was studied using electroantenography (EAG). Results showed that the three ligands are electrophysiologically active. However, an aliquot of β‐ionone (represented by 3.0 ng) elicited stronger EAG responses in antennae of males than of females. Finally, the role of these ligands as potential semiochemicals for H. elegans is discussed.

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“…Chemical perception in insects relies on olfaction and gustation, which enable insects to accomplish important tasks such as mating, host finding and predator avoidance in their environment (Sánchez-Gracia, Vieira & Rozas, 2009). Physically, these abilities depend on small, specialized organs called sensilla (Mutis et al, 2014), which populate insect’s appendices, such as antennae and palps. Sensilla are exposed to chemical stimuli and protect the neuronal dendrites inside (Suh, Bohbot & Zwiebel, 2014).…”

Section: Introductionmentioning

confidence: 99%

Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae)

González-González

Rubio-Meléndez

Ballesteros

et al. 2019

PeerJ

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In this study, we addressed the sex- and tissue-specific expression patterns of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in Hylamorpha elegans (Burmeister), an important native scarab beetle pest species from Chile. Similar to other members of its family, this scarab beetle exhibit habits that make difficult to control the pest by conventional methods. Hence, alternative ways to manage the pest populations based on chemical communication and signaling (such as disrupting mating or host finding process) are highly desirable. However, developing pest-control methods based on chemical communication requires to understand the molecular basis for pheromone recognition/chemical perception in this species. Thus, with the aim of discovering olfaction-related genes, we obtained the first reference transcriptome assembly of H. elegans. We used different tissues of adult beetles from males and females: antennae and maxillary palps, which are well known for embedded sensory organs. Then, the expression of predicted odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) was analyzed by qRT-PCR. In total, 165 transcripts related to chemoperception were predicted. Of these, 16 OBPs, including one pheromone-binding protein (PBP), and four CSPs were successfully amplified by qRT-PCR. All of these genes were differentially expressed in the sensory tissues with respect to the tibial tissue that was used as a control. The single predicted PBP found was highly expressed in the antennal tissues, particularly in males, while several OBPs and one CSP showed male-biased expression patterns, suggesting that these proteins may participate in sexual recognition process. In addition, a single CSP was expressed at higher levels in female palps than in any other studied condition, suggesting that this CSP would participate in oviposition process. Finally, all four CSPs exhibited palp-biased expression while mixed results were obtained for the expression of the OBPs, which were more abundant in the palps than in the antennae. These results suggest that these chemoperception proteins would be interesting novel targets for control of H. elegans, thus providing a theoretical basis for further studies involving new pest control methods.

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Morphology and Distribution of Sensilla on the Antennae of Hylamorpha elegans Burmeister (Coleoptera: Scarabaeidae)

Cited by 19 publications

References 26 publications

Antennal fine morphology of the threatened beetle Osmoderma eremita (Coleoptera: Scarabaeidae), revealed by scanning electron microscopy

Antennal fine morphology of the threatened beetle Osmoderma eremita (Coleoptera: Scarabaeidae), revealed by scanning electron microscopy

β‐Ionone as putative semiochemical suggested by ligand binding on an odorant‐binding protein of Hylamorpha elegans and electroantennographic recordings

Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae)

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